{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:29:47Z","timestamp":1760239787605,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>An 8-year-old alpaca was admitted to the emergency service of the Clinic for Ruminants in Bern due to a reduced general condition and progressive neurological signs. Despite supportive treatment, its condition deteriorated and the animal had to be euthanized. Histopathological analysis revealed a severe non-suppurative polioencephalomyelitis with neuronal necrosis, most likely of viral origin. We detected abundant neuronal labelling with antibodies directed against two different epitopes of Bovine Astrovirus CH13\/NeuroS1 (BoAstV-CH13\/NeuroS1), which is a common viral agent associated with non-suppurative encephalitis in Swiss cattle. These findings were further verified by detection of viral RNA by use of in-situ hybridization and real-time RT-PCR. Next generation sequencing revealed that the detected virus genome had a pairwise identity of 98.9% to the genome of BoAstV-CH13\/NeuroS1. To our knowledge, this is the first report of an astrovirus-associated polioencephalomyelitis in an alpaca. These results point to the possibility of an interspecies transmission of BoAstV-CH13\/NeuroS1.<\/jats:p>","DOI":"10.3390\/v13010050","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T20:13:41Z","timestamp":1609359221000},"page":"50","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Astrovirus-Associated Polioencephalomyelitis in an Alpaca"],"prefix":"10.3390","volume":"13","author":[{"given":"Leonore","family":"K\u00fcchler","sequence":"first","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse-Faculty, Universitiy of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1591-7690","authenticated-orcid":false,"given":"Isabelle","family":"R\u00fcfli","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel C.","family":"Koch","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie M.","family":"Hierweger","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronja V.","family":"Kauer","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line L.","family":"Boujon","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Hilbe","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Pathology, Vetsuisse-Faculty, University of Zurich, 8006 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3569-8547","authenticated-orcid":false,"given":"Anna","family":"Oevermann","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9549-2487","authenticated-orcid":false,"given":"Patrik","family":"Zanolari","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-5129","authenticated-orcid":false,"given":"Torsten","family":"Seuberlich","sequence":"additional","affiliation":[{"name":"Division of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Gurtner","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse-Faculty, Universitiy of Bern, 3012 Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.meegid.2011.07.024","article-title":"Astrovirus Infections in Humans and Animals","volume":"11","year":"2011","journal-title":"Infect. 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