{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:05:46Z","timestamp":1783735546489,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,17]],"date-time":"2021-01-17T00:00:00Z","timestamp":1610841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programa Polos de Desarrollo Universitario from Comisi\u00f3n Coordinadora del Interior of the Universidad de la Rep\u00fablica, Uruguay","award":["PDU2010"],"award-info":[{"award-number":["PDU2010"]}]},{"name":"National Agency for Scientific and Technological Promotion, Argentina, ANPCyT-FONCyT-PICT 2016","award":["2165"],"award-info":[{"award-number":["2165"]}]},{"name":"Sistema Nacional de Investigadores (SNI) of Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n (ANII), Uruguay","award":["SNI"],"award-info":[{"award-number":["SNI"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","award":["CONICET"],"award-info":[{"award-number":["CONICET"]}]},{"name":"Comisi\u00f3n Acad\u00e9mica de Posgrado (CAP), Universidad de la Rep\u00fablica, Uruguay","award":["PhD Scholarship"],"award-info":[{"award-number":["PhD Scholarship"]}]},{"name":"Programa de Desarrollo de las Ciencias B\u00e1sicas (PEDECIBA) of Universidad de la Rep\u00fablica, Uruguay","award":["PEDECIBA"],"award-info":[{"award-number":["PEDECIBA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The knowledge about circulation of Human Enteroviruses (EVs) obtained through medical diagnosis in Argentina is scarce. Wastewater samples monthly collected in C\u00f3rdoba, Argentina during 2011\u20132012, and then in 2017\u20132018 were retrospectively studied to assess the diversity of EVs in the community. Partial VP1 gene was amplified by PCR from wastewater concentrates, and amplicons were subject of next-generation sequencing and genetic analyses. There were 41 EVs detected, from which ~50% had not been previously reported in Argentina. Most of the characterized EVs (60%) were detected at both sampling periods, with similar values of intratype nucleotide diversity. Exceptions were enterovirus A71, coxsackievirus B4, echovirus 14, and echovirus 30, which diversified in 2017\u20132018. There was a predominance of types from EV-C in 2017\u20132018, evidencing a common circulation of these types throughout the year in the community. Interestingly, high genetic similarity was evidenced among environmental strains of echovirus 30 circulating in 2011\u20132012 and co-temporal isolates obtained from patients suffering aseptic meningitis in different locations of Argentina. This study provides an updated insight about EVs circulating in an important region of South America, and suggests a valuable role of wastewater-based epidemiology in predicting outbreaks before the onset of cases in the community.<\/jats:p>","DOI":"10.3390\/v13010120","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T02:36:05Z","timestamp":1611196565000},"page":"120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Environmental Surveillance through Next-Generation Sequencing to Unveil the Diversity of Human Enteroviruses beyond the Reported Clinical Cases"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-6916","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Lizasoain","sequence":"first","affiliation":[{"name":"Laboratorio de Virolog\u00eda Molecular, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional del Litoral Norte, Universidad de la Rep\u00fablica, Salto 50000, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiana","family":"Mir","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00f3mica y Bioinform\u00e1tica, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional del Litoral Norte, Universidad de la Rep\u00fablica, Salto 50000, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisella","family":"Masachessi","sequence":"additional","affiliation":[{"name":"Laboratorio de Gastroenteritis Virales y Sarampi\u00f3n, Instituto de Virolog\u00eda Dr. J. M. Vanella, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba 5000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri\u00e1n","family":"Far\u00edas","sequence":"additional","affiliation":[{"name":"Laboratorio de Gastroenteritis Virales y Sarampi\u00f3n, Instituto de Virolog\u00eda Dr. J. M. Vanella, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba 5000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-979X","authenticated-orcid":false,"given":"N\u00e9lida","family":"Rodr\u00edguez-Osorio","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00f3mica y Bioinform\u00e1tica, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional del Litoral Norte, Universidad de la Rep\u00fablica, Salto 50000, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas","family":"Victoria","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Molecular, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional del Litoral Norte, Universidad de la Rep\u00fablica, Salto 50000, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Nates","sequence":"additional","affiliation":[{"name":"Laboratorio de Gastroenteritis Virales y Sarampi\u00f3n, Instituto de Virolog\u00eda Dr. J. M. Vanella, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba 5000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney","family":"Colina","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Molecular, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional del Litoral Norte, Universidad de la Rep\u00fablica, Salto 50000, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1099\/jgv.0.000911","article-title":"ICTV Virus Taxonomy Profile: Picornaviridae","volume":"98","author":"Zell","year":"2017","journal-title":"J. Gen. 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