{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:11:36Z","timestamp":1768554696426,"version":"3.49.0"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["National Institutes of Health contract Nr. 75N9301900065"],"award-info":[{"award-number":["National Institutes of Health contract Nr. 75N9301900065"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["XXX"],"award-info":[{"award-number":["XXX"]}]},{"name":"Instituto Oswaldo Cruz\/ Funda\u00e7\u00e3o Oswaldo Cruz","award":["XXX"],"award-info":[{"award-number":["XXX"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>There have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough evaluation of the inflammatory and cytotoxic profiles as well as the expression of CCR5 and CX3CR1. We compared the reactivity of T cells to ZIKV peptides in convalescent mothers infected during pregnancy. The child\u2019s clinical outcome (i.e., born with or without CZS) was taken to be the variable. The cells were stimulated in vitro with ZIKV peptides and evaluated using the ELISPOT and flow cytometry assays. After in vitro stimulation with ZIKV peptides, we observed a tendency toward a higher Interferon gamma (IFN-\u03b3)-producing T cell responses in mothers who had asymptomatic children and a higher CD107a expression in T cells in mothers who had children with CZS. We found a higher frequency of T cells expressing CD107a+ and co-expressing CX3CR1+CCR5+, which is much clearer in the T cells of mothers who had CZS children. We suggest that this differential profile influenced the clinical outcome of babies. These data need to be further investigated, including the evaluation of other ZIKV peptides and markers and functional assays.<\/jats:p>","DOI":"10.3390\/v13020191","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T01:22:23Z","timestamp":1611796943000},"page":"191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9229-5912","authenticated-orcid":false,"given":"D\u00e9bora","family":"Familiar-Macedo","sequence":"first","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iury","family":"Amancio Paiva","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Badolato-Corr\u00eaa da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana Rabe","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Multiuser Laboratory for Research in Nephrology and Medical Science, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro 24 033-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helver Gon\u00e7alves","family":"Dias","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6924-157X","authenticated-orcid":false,"given":"Alex","family":"Pauvolid-Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences, Texas A&amp;M University, Texas, TX 77843, USA"},{"name":"Laboratory of Respiratory Viruses and Measles, SARS-CoV-2 National Reference Laboratory and Regional Reference Laboratory in Americas (PAHO\/WHO), Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline Fernandes","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3603-6921","authenticated-orcid":false,"given":"Mariana","family":"Gandini","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Microbiology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5856-6128","authenticated-orcid":false,"given":"Andr\u00e9a Alice","family":"Silva","sequence":"additional","affiliation":[{"name":"Multiuser Laboratory for Research in Nephrology and Medical Science, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro 24 033-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Maria","family":"Baeta Cavalcanti","sequence":"additional","affiliation":[{"name":"Laboratory of Virological Diagnosis, Biomedical Institute, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro 24 110-130, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1862-2348","authenticated-orcid":false,"given":"Solange","family":"Artimos de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro 24 033-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6416-7278","authenticated-orcid":false,"given":"Renata","family":"Artimos de Oliveira Vianna","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro 24 033-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elzinandes","family":"Leal de Azeredo","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 1040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-5966","authenticated-orcid":false,"given":"Alba","family":"Grifoni","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USA"},{"name":"Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego (UCSD), La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Weiskopf","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcv.2015.11.014","article-title":"First detection of autochthonous Zika virus transmission in a HIV-infected patient in Rio de Janeiro, Brazil","volume":"74","author":"Calvet","year":"2016","journal-title":"J. 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