{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:13:21Z","timestamp":1790298801668,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R15AI105737"],"award-info":[{"award-number":["R15AI105737"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM103546"],"award-info":[{"award-number":["P20GM103546"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["R03TR002937"],"award-info":[{"award-number":["R03TR002937"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In recent years, transcriptome profiling studies have identified changes in host splicing patterns caused by viral invasion, yet the functional consequences of the vast majority of these splicing events remain uncharacterized. We recently showed that the host splicing landscape changes during Rift Valley fever virus MP-12 strain (RVFV MP-12) infection of mammalian cells. Of particular interest, we observed that the host mRNA for Rio Kinase 3 (RIOK3) was alternatively spliced during infection. This kinase has been shown to be involved in pattern recognition receptor (PRR) signaling mediated by RIG-I like receptors to produce type-I interferon. Here, we characterize RIOK3 as an important component of the interferon signaling pathway during RVFV infection and demonstrate that RIOK3 mRNA expression is skewed shortly after infection to produce alternatively spliced variants that encode premature termination codons. This splicing event plays a critical role in regulation of the antiviral response. Interestingly, infection with other RNA viruses and transfection with nucleic acid-based RIG-I agonists also stimulated RIOK3 alternative splicing. Finally, we show that specifically stimulating alternative splicing of the RIOK3 transcript using a morpholino oligonucleotide reduced interferon expression. Collectively, these results indicate that RIOK3 is an important component of the mammalian interferon signaling cascade and its splicing is a potent regulatory mechanism capable of fine-tuning the host interferon response.<\/jats:p>","DOI":"10.3390\/v13030367","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T09:47:20Z","timestamp":1614332840000},"page":"367","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Atypical Kinase RIOK3 Limits RVFV Propagation and Is Regulated by Alternative Splicing"],"prefix":"10.3390","volume":"13","author":[{"given":"Katherine E.","family":"Havranek","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke Adam","family":"White","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Bisom","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Montana, Missoula, MT 59812, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Lanchy","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Stephen","family":"Lodmell","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA"},{"name":"Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, MT 59812, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1007\/s00705-017-3358-5","article-title":"Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses","volume":"162","author":"Adams","year":"2017","journal-title":"Arch. 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