{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:09:53Z","timestamp":1768810193824,"version":"3.49.0"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T00:00:00Z","timestamp":1614902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation, Sri Lanka","award":["RG\/2012\/BT\/03"],"award-info":[{"award-number":["RG\/2012\/BT\/03"]}]},{"DOI":"10.13039\/501100008816","name":"University of Sri Jayewardenepura","doi-asserted-by":"publisher","award":["ASP\/06\/RE\/SCI\/2013\/19"],"award-info":[{"award-number":["ASP\/06\/RE\/SCI\/2013\/19"]}],"id":[{"id":"10.13039\/501100008816","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Genetic variations in dengue virus (DENV) play a distinct role in epidemic emergence. The DENV 3\u2032 UTR has become a recent interest in research. The objective of the study was to examine the genetic variation in the domain II, 3\u2032 UTR region of human and mosquito-derived DENV. DENV-infected human sera were orally infected to laboratory reared Aedes aegypti mosquitoes. The domain II, 3\u2032 UTR of each human- and mosquito-derived sample was amplified. The nucleotide sequence variation, phylogenetic and secondary structure analysis was carried out incorporating respective regions of so far recorded Sri Lankan and the reference genotype strains of the DENV3 and DENV1 serotypes. The human- and mosquito-derived domain II, 3\u2032 UTR were identical in nucleotide sequences within the serotypes isolated, indicating the conserved nature of the region during host switch. The sequence analysis revealed distinct variations in study isolates compared to so far recorded Sri Lankan isolates. However, despite single nucleotide variations, the maintenance of structural integrity was evident in related strains within the serotypes in the secondary structure analysis. The phylogenetic analysis revealed distinct clade segregation of the study sequences from so far reported Sri Lankan isolates and illustrated the phylogenetic relations of the study sequences to the available global isolates of respective serotypes.<\/jats:p>","DOI":"10.3390\/v13030421","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T11:46:09Z","timestamp":1614944769000},"page":"421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genetic Variation in the Domain II, 3\u2032 Untranslated Region of Human and Mosquito Derived Dengue Virus Strains in Sri Lanka"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-7251","authenticated-orcid":false,"given":"P. D.","family":"Dayananda","sequence":"first","affiliation":[{"name":"Center for Biotechnology, Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harendra","family":"de Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, University of Colombo, Colombo 00700, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LakKumar","family":"Fernando","sequence":"additional","affiliation":[{"name":"Centre for Clinical Management of Dengue &amp; Dengue Hemorrhagic Fever, Negombo 11500, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. G. D. N. K.","family":"de Silva","sequence":"additional","affiliation":[{"name":"Center for Biotechnology, Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda 10250, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S1567-1348(03)00004-2","article-title":"The origin, emergence and evolutionary genetics of dengue virus","volume":"3","author":"Holmes","year":"2003","journal-title":"Infect. Genet. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/S1473-3099(16)00026-8","article-title":"The global burden of dengue: An analysis from the Global Burden of Disease Study 2013","volume":"16","author":"Stanaway","year":"2016","journal-title":"Lancet Infect. 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