{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:30:11Z","timestamp":1771007411167,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T00:00:00Z","timestamp":1620777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The aim of this study was to determine whether self-collected pure saliva (SCPS) is comparable to nasopharyngeal (NP) swabs in the quantitative detection of SARS-CoV-2 by RT-PCR in asymptomatic, mild patients with confirmed COVID-19. Thirty-one patients aged from 18 to 85 years were included between 9 June and 11 December 2020. A SCPS sample and a NP sample were taken for each patient. Quantitative PCR was performed to detect SARS-CoV-2 viral load. Results of SCPS vs. NP samples testing were compared. Statistical analyses were performed. Viral load was significantly correlated (r = 0.72). The concordance probability was estimated at 73.3%. In symptomatic adults, SCPS performance was similar to that of NP swabs (Percent Agreement = 74.1%; p = 0.11). Thus, the salivary test based on pure oral saliva samples easily obtained by noninvasive techniques has a fair agreement with the nasopharyngeal one in asymptomatic, mild patients with a confirmed diagnosis of COVID-19.<\/jats:p>","DOI":"10.3390\/v13050895","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T03:08:12Z","timestamp":1620875292000},"page":"895","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6823-1636","authenticated-orcid":false,"given":"Florence","family":"Carrouel","sequence":"first","affiliation":[{"name":"Laboratory \u201cHealth Systemic Process\u201d, EA4129, University Lyon 1, 69008 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Valette","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institute of Infectious Agents, Croix-Rousse Hospital, Hospices Civils of Lyon, 69004 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Perrier","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, Protestant Infirmary, 69300 Caluire-et-Cuire, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maude","family":"Bouscambert-Duchamp","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institute of Infectious Agents, Croix-Rousse Hospital, Hospices Civils of Lyon, 69004 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1091-9139","authenticated-orcid":false,"given":"Claude","family":"Dussart","sequence":"additional","affiliation":[{"name":"Laboratory \u201cHealth Systemic Process\u201d, EA4129, University Lyon 1, 69008 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Tramini","sequence":"additional","affiliation":[{"name":"Department of Public Health, Faculty of Dental Medicine, University of Montpellier, 34090 Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4754-2512","authenticated-orcid":false,"given":"Denis","family":"Bourgeois","sequence":"additional","affiliation":[{"name":"Laboratory \u201cHealth Systemic Process\u201d, EA4129, University Lyon 1, 69008 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yoon, J.G., Yoon, J., Song, J.Y., Yoon, S.Y., Lim, C.S., Seong, H., Noh, J.Y., Cheong, H.J., and Kim, W.J. (2020). Clinical Significance of a High SARS-CoV-2 Viral Load in the Saliva. J. Korean Med. Sci., 35.","DOI":"10.3346\/jkms.2020.35.e195"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"To, K.K.-W., Tsang, O.T.-Y., Chik-Yan Yip, C., Chan, K.-H., Wu, T.-C., Chan, J.M.C., Leung, W.-S., Chik, T.S.-H., Choi, C.Y.-C., and Kandamby, D.H. (2020). Consistent Detection of 2019 Novel Coronavirus in Saliva. Clin. Infect. Dis.","DOI":"10.1093\/cid\/ciaa149"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Yokota, I., Shane, P.Y., Okada, K., Unoki, Y., Yang, Y., Inao, T., Sakamaki, K., Iwasaki, S., Hayasaka, K., and Sugita, J. (2020). Mass Screening of Asymptomatic Persons for SARS-CoV-2 Using Saliva. Clin. Infect. Dis.","DOI":"10.1101\/2020.08.13.20174078"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1002\/jmv.26258","article-title":"Deep Throat Saliva as an Alternative Diagnostic Specimen Type for the Detection of SARS-CoV-2","volume":"93","author":"Leung","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Procop, G.W., Shrestha, N.K., Vogel, S., Van Sickle, K., Harrington, S., Rhoads, D.D., Rubin, B.P., and Terpeluk, P. (2020). A Direct Comparison of Enhanced Saliva to Nasopharyngeal Swab for the Detection of SARS-CoV-2 in Symptomatic Patients. J. Clin. Microbiol., 58.","DOI":"10.1128\/JCM.01946-20"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Michailidou, E., Poulopoulos, A., and Tzimagiorgis, G. (2020). Salivary Diagnostics of the Novel Coronavirus SARS-CoV-2 (COVID-19). Oral. Dis.","DOI":"10.1111\/odi.13729"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"62","DOI":"10.15406\/jlprr.2020.07.00230","article-title":"Simple Classification of COVID-19 Patients","volume":"7","year":"2020","journal-title":"J. Lung Pulm. Respir. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Carrouel, F., Viennot, S., Valette, M., Cohen, J.-M., Dussart, C., and Bourgeois, D. (2020). Salivary and Nasal Detection of the SARS-CoV-2 Virus After Antiviral Mouthrinses (BBCovid): A Structured Summary of a Study Protocol for a Randomised Controlled Trial. Trials, 21.","DOI":"10.1186\/s13063-020-04846-6"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1128\/JCM.02816-15","article-title":"Evaluation of a New Device for Simplifying and Standardizing Stool Sample Preparation for Viral Molecular Testing with Limited Hands-On Time","volume":"54","author":"Feghoul","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Migueres, M., Mengelle, C., Dimeglio, C., Didier, A., Alvarez, M., Delobel, P., Mansuy, J.-M., and Izopet, J. (2020). Saliva Sampling for Diagnosing SARS-CoV-2 Infections in Symptomatic Patients and Asymptomatic Carriers. J. Clin. Virol., 130.","DOI":"10.1016\/j.jcv.2020.104580"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"285.e1","DOI":"10.1016\/j.cmi.2020.05.001","article-title":"Saliva Sample as a Non-Invasive Specimen for the Diagnosis of Coronavirus Disease 2019: A Cross-Sectional Study","volume":"27","author":"Pasomsub","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1056\/NEJMc2016359","article-title":"Saliva or Nasopharyngeal Swab Specimens for Detection of SARS-CoV-2","volume":"383","author":"Wyllie","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Williams, E., Bond, K., Zhang, B., Putland, M., and Williamson, D.A. (2020). Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2. J. Clin. Microbiol., 58.","DOI":"10.1128\/JCM.00776-20"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.medj.2020.12.010","article-title":"SalivaDirect: A Simplified and Flexible Platform to Enhance SARS-CoV-2 Testing Capacity","volume":"2","author":"Vogels","year":"2021","journal-title":"Medicine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e145","DOI":"10.1016\/j.jinf.2020.05.071","article-title":"Comparison of SARS-CoV-2 Detection in Nasopharyngeal Swab and Saliva","volume":"81","author":"Iwasaki","year":"2020","journal-title":"J. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mahallawi, W.H., Alsamiri, A.D., Dabbour, A.F., Alsaeedi, H., and Al-Zalabani, A.H. (2021). Association of Viral Load in SARS-CoV-2 Patients With Age and Gender. Front. Med., 8.","DOI":"10.3389\/fmed.2021.608215"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1016\/S2666-5247(20)30172-5","article-title":"SARS-CoV-2, SARS-CoV, and MERS-CoV Viral Load Dynamics, Duration of Viral Shedding, and Infectiousness: A Systematic Review and Meta-Analysis","volume":"2","author":"Cevik","year":"2021","journal-title":"Lancet Microbe"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yokota, I., Hattori, T., Shane, P.Y., Konno, S., Nagasaka, A., Takeyabu, K., Fujisawa, S., Nishida, M., and Teshima, T. (2021). Equivalent SARS-CoV-2 Viral Loads by PCR between Nasopharyngeal Swab and Saliva in Symptomatic Patients. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-84059-2"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ca\u00f1ete, M.G., Valenzuela, I.M., Garc\u00e9s, P.C., Mass\u00f3, I.C., Gonz\u00e1lez, M.J., and Providell, S.G. (2021). Saliva Sample for the Massive Screening of SARS-CoV-2 Infection: A Systematic Review. Oral. Surg. Oral. Med. Oral. Pathol. Oral. Radiol.","DOI":"10.1016\/j.oooo.2021.01.028"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1001\/jamainternmed.2020.8876","article-title":"Comparison of Saliva and Nasopharyngeal Swab Nucleic Acid Amplification Testing for Detection of SARS-CoV-2: A Systematic Review and Meta-Analysis","volume":"181","author":"Lawandi","year":"2021","journal-title":"JAMA Intern. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bastos, M.L., Perlman-Arrow, S., Menzies, D., and Campbell, J.R. (2021). The Sensitivity and Costs of Testing for SARS-CoV-2 Infection With Saliva Versus Nasopharyngeal Swabs: A Systematic Review and Meta-Analysis. Ann. Intern. Med.","DOI":"10.7326\/M20-6569"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/5\/895\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:59:45Z","timestamp":1760162385000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/5\/895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,12]]},"references-count":21,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["v13050895"],"URL":"https:\/\/doi.org\/10.3390\/v13050895","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,12]]}}}