{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:37:54Z","timestamp":1790062674502,"version":"4.0.1"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001323","name":"Poliomyelitis Research Foundation","doi-asserted-by":"publisher","award":["17\/37"],"award-info":[{"award-number":["17\/37"]}],"id":[{"id":"10.13039\/501100001323","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Research Foundation South Africa","award":["107424 and 116385"],"award-info":[{"award-number":["107424 and 116385"]}]},{"name":"German Federal Ministry of Education and Research","award":["VN81204343"],"award-info":[{"award-number":["VN81204343"]}]},{"name":"G7 Global Health Protection program","award":["FKZ1368-1438TO08"],"award-info":[{"award-number":["FKZ1368-1438TO08"]}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["1U19GH000571-01-GDD"],"award-info":[{"award-number":["1U19GH000571-01-GDD"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Orthobunyavirus genus, family Peribunyaviridae, contains several important emerging and re-emerging arboviruses of veterinary and medical importance. These viruses may cause mild febrile illness, to severe encephalitis, fetal deformity, abortion, hemorrhagic fever and death in humans and\/or animals. Shuni virus (SHUV) is a zoonotic arbovirus thought to be transmitted by hematophagous arthropods. It was previously reported in a child in Nigeria in 1966 and horses in Southern Africa in the 1970s and again in 2009, and in humans with neurological signs in 2017. Here we investigated the epidemiology and phylogenetic relationship of SHUV strains detected in horses presenting with febrile and neurological signs in South Africa. In total, 24\/1820 (1.3%) horses submitted to the zoonotic arbovirus surveillance program tested positive by real-time reverse transcription (RTPCR) between 2009 and 2019. Cases were detected in all provinces with most occurring in Gauteng (9\/24, 37.5%). Neurological signs occurred in 21\/24 (87.5%) with a fatality rate of 45.8%. Partial sequencing of the nucleocapsid gene clustered the identified strains with SHUV strains previously identified in South Africa (SA). Full genome sequencing of a neurological case detected in 2016 showed 97.8% similarity to the SHUV SA strain (SAE18\/09) and 97.5% with the Nigerian strain and 97.1% to the 2014 Israeli strain. Our findings suggest that SHUV is circulating annually in SA and despite it being relatively rare, it causes severe neurological disease and death in horses.<\/jats:p>","DOI":"10.3390\/v13050937","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T21:49:21Z","timestamp":1621460961000},"page":"937","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Epidemiology of Shuni Virus in Horses in South Africa"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4157-4126","authenticated-orcid":false,"given":"Thopisang P.","family":"Motlou","sequence":"first","affiliation":[{"name":"Zoonotic Arbo and Respiratory Virus Program, Centre for Viral Zoonoses, Department Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria 0031, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Section Pathology, Faculty of Veterinary Science, University of Pretoria, Pretoria 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2696-824X","authenticated-orcid":false,"given":"Marietjie","family":"Venter","sequence":"additional","affiliation":[{"name":"Zoonotic Arbo and Respiratory Virus Program, Centre for Viral Zoonoses, Department Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria 0031, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kolakofsky, D. (1991). Bunyaviridae\u2014Natural History. Bunyaviridae, Springer.","DOI":"10.1007\/978-3-642-76018-1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1007\/s11481-010-9234-7","article-title":"Arboviral encephalitides: Transmission, emergence, and pathogenesis","volume":"5","author":"Hollidge","year":"2010","journal-title":"J. Neuroimmune Pharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jviromet.2014.07.030","article-title":"Development of an algorithm for production of inactivated arbovirus antigens in cell culture","volume":"208","author":"Goodman","year":"2014","journal-title":"J. Virol. Methods"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1099\/0022-1317-71-3-501","article-title":"Molecular biology of the Bunyaviridae","volume":"71","author":"Elliott","year":"1990","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1159\/000149174","article-title":"Bunyaviridae","volume":"14","author":"Bishop","year":"1980","journal-title":"Intervirology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1099\/0022-1317-82-9-2173","article-title":"Phylogeny of the Simbu serogroup of the genus Bunyavirus","volume":"82","author":"Saeed","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s00705-003-0266-7","article-title":"The emergence in Japan of Sathuperi virus, a tropical Simbu serogroup virus of the genus Orthobunyavirus","volume":"149","author":"Yanase","year":"2004","journal-title":"Arch. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8922","DOI":"10.1128\/JVI.78.16.8922-8926.2004","article-title":"Ngari virus is a Bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in Africa","volume":"78","author":"Gerrard","year":"2004","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Elliott, R.M. (1996). History, Classification, and Taxonomy of Viruses in the Family Bunyaviridae. The Bunyaviridae, Springer.","DOI":"10.1007\/978-1-4899-1364-7"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"469","DOI":"10.3201\/eid1803.111905","article-title":"Novel orthobunyavirus in cattle, Europe, 2011","volume":"18","author":"Hoffmann","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Varela, M., Schnettler, E., Caporale, M., Murgia, C., Barry, G., McFarlane, M., McGregor, E., Piras, I.M., Shaw, A., and Lamm, C. (2013). Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003133"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Aguilar, P.V., Barrett, A.D., Saeed, M.F., Watts, D.M., Russell, K., Guevara, C., Ampuero, J.S., Suarez, L., Cespedes, M., and Montgomery, J.M. (2011). Iquitos virus: A novel reassortant Orthobunyavirus associated with human illness in Peru. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001315"},{"key":"ref_13","first-page":"249","article-title":"Arbovirus surveillance in Nigeria, 1964\u20131967","volume":"62","author":"Causey","year":"1968","journal-title":"Bull. Soc. Pathol. Exot. Fil."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/00034983.1975.11686983","article-title":"Arthropod-borne viral infections of man in Nigeria, 1964\u20131970","volume":"69","author":"Moore","year":"1975","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1093\/jmedent\/9.2.155","article-title":"Further isolations of arboviruses from mosquitoes collected in Tongaland, South Africa, 1960\u20131968","volume":"9","author":"Mclntosh","year":"1972","journal-title":"J. Med Entomol."},{"key":"ref_16","first-page":"803","article-title":"Rift Valley fever in humans in South Africa","volume":"58","author":"McIntosh","year":"1980","journal-title":"S. Afr. Med. J.\/Suid-Afrikaanse Mediese Tydskrift"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"318","DOI":"10.3201\/eid1802.111403","article-title":"Shuni virus as cause of neurologic disease in horses","volume":"18","author":"Williams","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.3201\/eid2008.131724","article-title":"Antibodies against West Nile and Shuni viruses in veterinarians, South Africa","volume":"20","author":"Swanepoel","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3201\/eid2702.191551","article-title":"Shuni Virus in Cases of Neurologic Disease in Humans, South Africa","volume":"27","author":"Motlou","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.3201\/eid2112.150804","article-title":"Malformations caused by Shuni virus in ruminants, Israel, 2014\u20132015","volume":"21","author":"Golender","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1111\/tbed.13167","article-title":"Shuni virus in Israel: Neurological disease and fatalities in cattle","volume":"66","author":"Golender","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_22","unstructured":"McIntosh, B. (1980). The Epidemiology of Arthropod-Borne Viruses in Southern Africa. [Ph.D. Thesis, University of Pretoria]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.coviro.2017.11.004","article-title":"Assessing the zoonotic potential of arboviruses of African origin","volume":"28","author":"Venter","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Steyn, J.M.T., Van Eeden, C., Pretorius, M., Williams, J., Buss, P., Beechler, B., Jolles, A., Perez-Martin, E., Myburgh, J.G., and Steyl, J. (2019). Shuni virus in wildlife and non-equine domestic animals in South Africa. Emerg. Infect. Dis., submitted.","DOI":"10.3201\/eid2607.190770"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jviromet.2013.10.008","article-title":"A sensitive nested real-time RT-PCR for the detection of Shuni Virus","volume":"195","author":"Zaayman","year":"2014","journal-title":"J. Virol. Methods"},{"key":"ref_26","unstructured":"Hall, T.A. (1999). BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT, Information Retrieval Ltd."},{"key":"ref_27","first-page":"4673","article-title":"ClustalW","volume":"22","author":"Thomopson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1128\/CMR.00002-06","article-title":"Role of cell culture for virus detection in the age of technology","volume":"20","author":"Leland","year":"2007","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Jansen van Vuren, P., Wiley, M., Palacios, G., Storm, N., McCulloch, S., Markotter, W., Birkhead, M., Kemp, A., and Paweska, J. (2016). Isolation of a novel fusogenic orthoreovirus from Eucampsipoda africana bat flies in South Africa. Viruses, 8.","DOI":"10.3390\/v8030065"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1093\/bib\/bbx108","article-title":"MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization","volume":"20","author":"Katoh","year":"2019","journal-title":"Brief. Bioinform."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.3201\/eid2312.162078","article-title":"West Nile virus lineage 2 in horses and other animals with neurologic disease, South Africa, 2008\u20132015","volume":"23","author":"Venter","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.3201\/eid1912.121527","article-title":"Epidemiologic investigations into outbreaks of Rift Valley fever in humans, South Africa, 2008\u20132011","volume":"19","author":"Archer","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1186\/s13071-018-2824-6","article-title":"Mosquito community composition in South Africa and some neighboring countries","volume":"11","author":"Cornel","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2883","DOI":"10.1007\/s00705-014-2131-2","article-title":"Genomic and phylogenetic characterization of Shuni virus","volume":"159","author":"Harders","year":"2014","journal-title":"Arch. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/5\/937\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:03:45Z","timestamp":1760162625000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/5\/937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,19]]},"references-count":35,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["v13050937"],"URL":"https:\/\/doi.org\/10.3390\/v13050937","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,19]]}}}