{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:17:19Z","timestamp":1769638639812,"version":"3.49.0"},"reference-count":15,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,5]],"date-time":"2021-06-05T00:00:00Z","timestamp":1622851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs, UK Government","doi-asserted-by":"publisher","award":["SE4113, SE4116, SE0550"],"award-info":[{"award-number":["SE4113, SE4116, SE0550"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rift Valley fever virus (RVFV) causes a zoonotic mosquito-borne haemorrhagic disease that emerges to produce rapid large-scale outbreaks in livestock within sub-Saharan Africa. A range of mosquito species in Africa have been shown to transmit RVFV, and recent studies have assessed whether temperate mosquito species are also capable of transmission. In order to support vector competence studies, the ability to visualize virus localization in mosquito cells and tissue would enhance the understanding of the infection process within the mosquito body. Here, the application of in situ hybridization utilizing RNAscope\u00ae to detect RVFV infection within the mosquito species, Culex pipiens, derived from the United Kingdom was demonstrated. Extensive RVFV replication was detected in many tissues of the mosquito with the notable exception of the interior of ovarian follicles.<\/jats:p>","DOI":"10.3390\/v13061079","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T22:23:00Z","timestamp":1623104580000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of Rift Valley Fever Virus RNA in Formalin-Fixed Mosquitoes by In Situ Hybridization (RNAscope\u00ae)"],"prefix":"10.3390","volume":"13","author":[{"given":"Sarah","family":"Lumley","sequence":"first","affiliation":[{"name":"Microbiology Services Division, Public Health England, Wiltshire SP4 0JG, UK"},{"name":"School of Veterinary Medicine, University of Surrey, Guildford GU2 7XH, UK"},{"name":"Virology Department, Animal and Plant Health Agency (Weybridge), Woodham Lane, Surrey KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Hunter","sequence":"additional","affiliation":[{"name":"Microbiology Services Division, Public Health England, Wiltshire SP4 0JG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6529-4367","authenticated-orcid":false,"given":"Kirsty","family":"Emery","sequence":"additional","affiliation":[{"name":"Microbiology Services Division, Public Health England, Wiltshire SP4 0JG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Hewson","sequence":"additional","affiliation":[{"name":"Microbiology Services Division, Public Health England, Wiltshire SP4 0JG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3243-6154","authenticated-orcid":false,"given":"Anthony R.","family":"Fooks","sequence":"additional","affiliation":[{"name":"Virology Department, Animal and Plant Health Agency (Weybridge), Woodham Lane, Surrey KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Horton","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of Surrey, Guildford GU2 7XH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6106-9373","authenticated-orcid":false,"given":"Nicholas","family":"Johnson","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of Surrey, Guildford GU2 7XH, UK"},{"name":"Virology Department, Animal and Plant Health Agency (Weybridge), Woodham Lane, Surrey KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5520","DOI":"10.1016\/j.vaccine.2015.08.020","article-title":"Rift Valley fever virus: A review of diagnosis and vaccination, and implications for emergence in Europe","volume":"33","author":"Mansfield","year":"2015","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1086\/378747","article-title":"Rift Valley fever epidemic in Saudi Arabia: Epidemiological, clinical and laboratory characteristics","volume":"37","author":"Madani","year":"2003","journal-title":"Clin. 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