{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:06:12Z","timestamp":1790028372335,"version":"4.0.1"},"reference-count":66,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R37AI147868"],"award-info":[{"award-number":["R37AI147868"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["RO1AI148784"],"award-info":[{"award-number":["RO1AI148784"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Host plasma membrane protein SERINC5 is incorporated into budding retrovirus particles where it blocks subsequent entry into susceptible target cells. Three structurally unrelated proteins encoded by diverse retroviruses, human immunodeficiency virus type 1 (HIV-1) Nef, equine infectious anemia virus (EIAV) S2, and ecotropic murine leukemia virus (MLV) GlycoGag, disrupt SERINC5 antiviral activity by redirecting SERINC5 from the site of virion assembly on the plasma membrane to an internal RAB7+ endosomal compartment. Pseudotyping retroviruses with particular glycoproteins, e.g., vesicular stomatitis virus glycoprotein (VSV G), renders the infectivity of particles resistant to inhibition by virion-associated SERINC5. To better understand viral determinants for SERINC5-sensitivity, the effect of SERINC5 was assessed using HIV-1, MLV, and Mason-Pfizer monkey virus (M-PMV) virion cores, pseudotyped with glycoproteins from Arenavirus, Coronavirus, Filovirus, Rhabdovirus, Paramyxovirus, and Orthomyxovirus genera. SERINC5 restricted virions pseudotyped with glycoproteins from several retroviruses, an orthomyxovirus, a rhabdovirus, a paramyxovirus, and an arenavirus. Infectivity of particles pseudotyped with HIV-1, amphotropic-MLV (A-MLV), or influenza A virus (IAV) glycoproteins, was decreased by SERINC5, whether the core was provided by HIV-1, MLV, or M-PMV. In contrast, particles pseudotyped with glycoproteins from M-PMV, parainfluenza virus 5 (PIV5), or rabies virus (RABV) were sensitive to SERINC5, but only with particular retroviral cores. Resistance to SERINC5 did not correlate with reduced SERINC5 incorporation into particles, route of viral entry, or absolute infectivity of the pseudotyped virions. These findings indicate that some non-retroviruses may be sensitive to SERINC5 and that, in addition to the viral glycoprotein, the retroviral core influences sensitivity to SERINC5.<\/jats:p>","DOI":"10.3390\/v13071279","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T02:44:39Z","timestamp":1625107479000},"page":"1279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Influence of Different Glycoproteins and of the Virion Core on SERINC5 Antiviral Activity"],"prefix":"10.3390","volume":"13","author":[{"given":"William E.","family":"Diehl","sequence":"first","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9080-7128","authenticated-orcid":false,"given":"Mehmet H.","family":"Guney","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9923-4138","authenticated-orcid":false,"given":"Teresa","family":"Vanzo","sequence":"additional","affiliation":[{"name":"Department of Cellular, Computational and Integrative Biology, University of Trento, 38123 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8259-6017","authenticated-orcid":false,"given":"Pyae P.","family":"Kyawe","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0532-996X","authenticated-orcid":false,"given":"Judith M.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9491-1483","authenticated-orcid":false,"given":"Massimo","family":"Pizzato","sequence":"additional","affiliation":[{"name":"Department of Cellular, Computational and Integrative Biology, University of Trento, 38123 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-4054","authenticated-orcid":false,"given":"Jeremy","family":"Luban","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA"},{"name":"Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1126\/science.270.5238.988","article-title":"Genomic Structure of an Attenuated Quasi Species of HIV-1 from a Blood Transfusion Donor and Recipients","volume":"270","author":"Deacon","year":"1995","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/0092-8674(91)90097-I","article-title":"Importance of the Nef Gene for Maintenance of High Virus Loads and for Development of AIDS","volume":"65","author":"Kestler","year":"1991","journal-title":"Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1056\/NEJM199501263320405","article-title":"Absence of Intact Nef Sequences in a Long-Term Survivor with Nonprogressive HIV-1 Infection","volume":"332","author":"Kirchhoff","year":"1995","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4923","DOI":"10.1128\/jvi.67.8.4923-4931.1993","article-title":"Nef from Primary Isolates of Human Immunodeficiency Virus Type 1 Suppresses Surface CD4 Expression in Human and Mouse T Cells","volume":"67","author":"Anderson","year":"1993","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5549","DOI":"10.1073\/pnas.90.12.5549","article-title":"CD4 down-Regulation by Nef Alleles Isolated from Human Immunodeficiency Virus Type 1-Infected Individuals","volume":"90","author":"Mariani","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/350508a0","article-title":"Serine Phosphorylation-Independent Downregulation of Cell-Surface CD4 by Nef","volume":"350","author":"Garcia","year":"1991","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11422","DOI":"10.1128\/JVI.79.17.11422-11433.2005","article-title":"Human Immunodeficiency Virus Nef Induces Rapid Internalization of the T-Cell Coreceptor CD8alphabeta","volume":"79","author":"Stove","year":"2005","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/nm0396-338","article-title":"Endocytosis of Major Histocompatibility Complex Class I Molecules Is Induced by the HIV-1 Nef Protein","volume":"2","author":"Schwartz","year":"1996","journal-title":"Nat. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Schindler, M., Schm\u00f6kel, J., Specht, A., Li, H., M\u00fcnch, J., Khalid, M., Sodora, D.L., Hahn, B.H., Silvestri, G., and Kirchhoff, F. (2008). Inefficient Nef-Mediated Downmodulation of CD3 and MHC-I Correlates with Loss of CD4+T Cells in Natural SIV Infection. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000107"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2906","DOI":"10.1128\/jvi.68.5.2906-2914.1994","article-title":"Optimal Infectivity in Vitro of Human Immunodeficiency Virus Type 1 Requires an Intact Nef Gene","volume":"68","author":"Chowers","year":"1994","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1128\/JVI.75.6.2993-3000.2001","article-title":"Human Immunodeficiency Virus Type 1 Nef Functions at the Level of Virus Entry by Enhancing Cytoplasmic Delivery of Virions","volume":"75","author":"Schaeffer","year":"2001","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10645","DOI":"10.1128\/JVI.77.19.10645-10650.2003","article-title":"Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells","volume":"77","author":"Tobiume","year":"2003","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5745","DOI":"10.1128\/JVI.78.11.5745-5755.2004","article-title":"Disruption of the Actin Cytoskeleton Can Complement the Ability of Nef to Enhance Human Immunodeficiency Virus Type 1 Infectivity","volume":"78","author":"Campbell","year":"2004","journal-title":"J. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.virol.2004.07.015","article-title":"HIV-1 Virion Fusion Assay: Uncoating Not Required and No Effect of Nef on Fusion","volume":"328","author":"Cavrois","year":"2004","journal-title":"Virology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1128\/JVI.78.3.1069-1079.2004","article-title":"The Membrane-Proximal Tyrosine-Based Sorting Signal of Human Immunodeficiency Virus Type 1 gp41 Is Required for Optimal Viral Infectivity","volume":"78","author":"Day","year":"2004","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1128\/jvi.69.7.4053-4059.1995","article-title":"Human Immunodeficiency Virus Type 1 Nef Increases the Efficiency of Reverse Transcription in the Infected Cell","volume":"69","author":"Schwartz","year":"1995","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5048","DOI":"10.1128\/jvi.69.8.5048-5056.1995","article-title":"Nef Stimulates Human Immunodeficiency Virus Type 1 Proviral DNA Synthesis","volume":"69","author":"Aiken","year":"1995","journal-title":"J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/nature15399","article-title":"HIV-1 Nef Promotes Infection by Excluding SERINC5 from Virion Incorporation","volume":"526","author":"Rosa","year":"2015","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/nature15400","article-title":"SERINC3 and SERINC5 Restrict HIV-1 Infectivity and Are Counteracted by Nef","volume":"526","author":"Usami","year":"2015","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6014","DOI":"10.1074\/jbc.M117.777714","article-title":"SERINC5 Protein Inhibits HIV-1 Fusion Pore Formation by Promoting Functional Inactivation of Envelope Glycoproteins","volume":"292","author":"Sood","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10915","DOI":"10.1128\/JVI.01246-16","article-title":"The Antagonism of HIV-1 Nef to SERINC5 Particle Infectivity Restriction Involves the Counteraction of Virion-Associated Pools of the Restriction Factor","volume":"90","author":"Trautz","year":"2016","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.chom.2016.08.004","article-title":"The Potency of Nef-Mediated SERINC5 Antagonism Correlates with the Prevalence of Primate Lentiviruses in the Wild","volume":"20","author":"Heigele","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13197","DOI":"10.1073\/pnas.1612044113","article-title":"S2 from Equine Infectious Anemia Virus Is an Infectivity Factor Which Counteracts the Retroviral Inhibitors SERINC5 and SERINC3","volume":"113","author":"Chande","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9364","DOI":"10.1073\/pnas.1001554107","article-title":"MLV Glycosylated-Gag Is an Infectivity Factor That Rescues Nef-Deficient HIV-1","volume":"107","author":"Pizzato","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e01985-16","DOI":"10.1128\/mBio.01985-16","article-title":"Functional Interplay Between Murine Leukemia Virus Glycogag, Serinc5, and Surface Glycoprotein Governs Virus Entry, with Opposite Effects on Gammaretroviral and Ebolavirus Glycoproteins","volume":"7","author":"Ahi","year":"2016","journal-title":"MBio"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15183","DOI":"10.1074\/jbc.RA120.014466","article-title":"HIV-Cell Membrane Fusion Intermediates Are Restricted by Serincs as Revealed by Cryo-Electron and TIRF Microscopy","volume":"295","author":"Ward","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lai, R.P.J., Yan, J., Heeney, J., McClure, M.O., G\u00f6ttlinger, H., Luban, J., and Pizzato, M. (2011). Nef Decreases HIV-1 Sensitivity to Neutralizing Antibodies That Target the Membrane-Proximal External Region of TMgp41. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002442"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.celrep.2013.09.028","article-title":"HIV-1 Nef Responsiveness Is Determined by Env Variable Regions Involved in Trimer Association and Correlates with Neutralization Sensitivity","volume":"5","author":"Usami","year":"2013","journal-title":"Cell Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e00588-20","DOI":"10.1128\/mBio.00588-20","article-title":"SERINC5 Potently Restricts Retrovirus Infection In Vivo","volume":"11","author":"Timilsina","year":"2020","journal-title":"MBio"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0042-6822(92)90692-I","article-title":"Retained in Vitro Infectivity and Cytopathogenicity of HIV-1 despite Truncation of the C-Terminal Tail of the Env Gene Product","volume":"189","author":"Wilk","year":"1992","journal-title":"Virology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1128\/AAC.46.6.1896-1905.2002","article-title":"Emergence of Resistant Human Immunodeficiency Virus Type 1 in Patients Receiving Fusion Inhibitor (T-20) Monotherapy","volume":"46","author":"Wei","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"11140","DOI":"10.1128\/JVI.00269-08","article-title":"Identification of Postentry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells","volume":"82","author":"Diehl","year":"2008","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1006\/mthe.2002.0585","article-title":"Lentiviral Vectors for Sustained Transgene Expression in Human Bone Marrow-Derived Stromal Cells","volume":"5","author":"Zhang","year":"2002","journal-title":"Mol. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"19134","DOI":"10.1073\/pnas.0605838103","article-title":"Balancing Selection and the Evolution of Functional Polymorphism in Old World Monkey TRIM5alpha","volume":"103","author":"Newman","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5670","DOI":"10.1128\/JVI.78.11.5670-5678.2004","article-title":"Capsid Is a Dominant Determinant of Retrovirus Infectivity in Nondividing Cells","volume":"78","author":"Yamashita","year":"2004","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1073\/pnas.86.5.1624","article-title":"Generation of Human Monoclonal Antibodies to Human Immunodeficiency Virus","volume":"86","author":"Gorny","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0042-6822(83)90378-1","article-title":"Characterization of Monoclonal Antibodies Reactive with Murine Leukemia Viruses: Use in Analysis of Strains of Friend MCF and Friend Ecotropic Murine Leukemia Virus","volume":"127","author":"Chesebro","year":"1983","journal-title":"Virology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1023\/B:EEST.0000011364.71236.f8","article-title":"Random Denominators and the Analysis of Ratio Data","volume":"11","author":"Liermann","year":"2004","journal-title":"Environ. Ecol. Stat."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.tim.2014.04.012","article-title":"The role of matrix in HIV-1 envelope glycoprotein incorporation","volume":"22","author":"Tedbury","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/jvi.57.2.603-613.1986","article-title":"Variant Influenza Virus Hemagglutinin That Induces Fusion at Elevated pH","volume":"57","author":"Doms","year":"1986","journal-title":"J. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"11637","DOI":"10.1128\/JVI.01648-13","article-title":"Uptake of Rabies Virus into Epithelial Cells by Clathrin-Mediated Endocytosis Depends upon Actin","volume":"87","author":"Piccinotti","year":"2013","journal-title":"J. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1006\/viro.1994.1057","article-title":"Acidic pH Triggers LCMV Membrane Fusion Activity and Conformational Change in the Glycoprotein Spike","volume":"198","author":"Zandonatti","year":"1994","journal-title":"Virology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/0092-8674(87)90542-3","article-title":"pH-Independent HIV Entry into CD4-Positive T Cells via Virus Envelope Fusion to the Plasma Membrane","volume":"49","author":"Stein","year":"1987","journal-title":"Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1002\/j.1460-2075.1988.tb02839.x","article-title":"Human Immunodeficiency Virus Infection of CD4-Bearing Cells Occurs by a pH-Independent Mechanism","volume":"7","author":"McClure","year":"1988","journal-title":"EMBO J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1099\/0022-1317-71-4-767","article-title":"The pH Independence of Mammalian Retrovirus Infection","volume":"71","author":"McClure","year":"1990","journal-title":"J. Gen. Virol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1128\/JVI.80.6.3071-3077.2006","article-title":"Analysis of the pH Requirement for Membrane Fusion of Different Isolates of the Paramyxovirus Parainfluenza Virus 5","volume":"80","author":"Bissonnette","year":"2006","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1126\/science.1110656","article-title":"Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection","volume":"308","author":"Chandran","year":"2005","journal-title":"Science"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.virol.2011.11.031","article-title":"Cathepsins B and L Activate Ebola but Not Marburg Virus Glycoproteins for Efficient Entry into Cell Lines and Macrophages Independent of TMPRSS2 Expression","volume":"424","author":"Gnirss","year":"2012","journal-title":"Virology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/nature10348","article-title":"Ebola Virus Entry Requires the Cholesterol Transporter Niemann-Pick C1","volume":"477","author":"Carette","year":"2011","journal-title":"Nature"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nature10380","article-title":"Small Molecule Inhibitors Reveal Niemann-Pick C1 Is Essential for Ebola Virus Infection","volume":"477","author":"Misasi","year":"2011","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1016\/j.cell.2016.10.014","article-title":"Ebola Virus Glycoprotein with Increased Infectivity Dominated the 2013-2016 Epidemic","volume":"167","author":"Diehl","year":"2016","journal-title":"Cell"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.cell.2016.10.013","article-title":"Human Adaptation of Ebola Virus during the West African Outbreak","volume":"167","author":"Urbanowicz","year":"2016","journal-title":"Cell"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11966","DOI":"10.1128\/JVI.01515-09","article-title":"Antagonism to and Intracellular Sequestration of Human Tetherin by the Human Immunodeficiency Virus Type 2 Envelope Glycoprotein","volume":"83","author":"Neil","year":"2009","journal-title":"J. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4060","DOI":"10.1128\/JVI.02425-08","article-title":"Foreign Glycoproteins Can Be Actively Recruited to Virus Assembly Sites during Pseudotyping","volume":"83","author":"Jorgenson","year":"2009","journal-title":"J. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1128\/jvi.70.1.341-351.1996","article-title":"Domains of the Human Immunodeficiency Virus Type 1 Matrix and gp41 Cytoplasmic Tail Required for Envelope Incorporation into Virions","volume":"70","author":"Freed","year":"1996","journal-title":"J. Virol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3338","DOI":"10.1128\/JVI.76.7.3338-3349.2002","article-title":"Mutation of the Dominant Endocytosis Motif in Human Immunodeficiency Virus Type 1 gp41 Can Complement Matrix Mutations without Increasing Env Incorporation","volume":"76","author":"West","year":"2002","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4283","DOI":"10.1128\/jvi.70.7.4283-4290.1996","article-title":"Producer-Cell Modification of Human Immunodeficiency Virus Type 1: Nef Is a Virion Protein","volume":"70","author":"Pandori","year":"1996","journal-title":"J. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5871","DOI":"10.1128\/jvi.71.8.5871-5877.1997","article-title":"Pseudotyping Human Immunodeficiency Virus Type 1 (HIV-1) by the Glycoprotein of Vesicular Stomatitis Virus Targets HIV-1 Entry to an Endocytic Pathway and Suppresses Both the Requirement for Nef and the Sensitivity to Cyclosporin A","volume":"71","author":"Aiken","year":"1997","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4014","DOI":"10.1128\/JVI.75.8.4014-4018.2001","article-title":"Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity","volume":"75","author":"Chazal","year":"2001","journal-title":"J. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1128\/JVI.76.11.5315-5325.2002","article-title":"Envelope Glycoprotein Incorporation, Not Shedding of Surface Envelope Glycoprotein (gp120\/SU), Is the Primary Determinant of SU Content of Purified Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus","volume":"76","author":"Chertova","year":"2002","journal-title":"J. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Brandenberg, O.F., Magnus, C., Rusert, P., Regoes, R.R., and Trkola, A. (2015). Different Infectivity of HIV-1 Strains Is Linked to Number of Envelope Trimers Required for Entry. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004595"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"13702","DOI":"10.1074\/jbc.M117.797332","article-title":"The Host-Cell Restriction Factor SERINC5 Restricts HIV-1 Infectivity without Altering the Lipid Composition and Organization of Viral Particles","volume":"292","author":"Trautz","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"11228","DOI":"10.1128\/JVI.00981-08","article-title":"Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides","volume":"82","author":"Chan","year":"2008","journal-title":"J. Virol."},{"key":"ref_64","first-page":"2081","article-title":"A New Virus in a Spontaneous Mammary Tumor of a Rhesus Monkey","volume":"30","author":"Chopra","year":"1970","journal-title":"Cancer Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1034\/j.1600-0854.2003.00125.x","article-title":"The M-PMV Cytoplasmic Targeting-Retention Signal Directs Nascent Gag Polypeptides to a Pericentriolar Region of the Cell","volume":"4","author":"Sfakianos","year":"2003","journal-title":"Traffic"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1021\/acsinfecdis.5b00040","article-title":"Lipid composition of viral envelope of three strains of influenza virus\u2014Not all viruses are created equal","volume":"1","author":"Ivanova","year":"2015","journal-title":"ACS Infect. 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