{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:08:45Z","timestamp":1760234925983,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["75N9301900065","U19AI135972","1K23AI143967"],"award-info":[{"award-number":["75N9301900065","U19AI135972","1K23AI143967"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo de Chile","award":["FONDECYT 1161971, PIA ACT 1408"],"award-info":[{"award-number":["FONDECYT 1161971, PIA ACT 1408"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["439967\/2016-3"],"award-info":[{"award-number":["439967\/2016-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","award":["ID-OAA-F-17-00084"],"award-info":[{"award-number":["ID-OAA-F-17-00084"]}],"id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Zika virus (ZIKV) is a mosquito-borne Flavivirus with a positive-sense RNA genome, which are generally transmitted through the bite of an infected Aedes mosquito. ZIKV infections could be associated with neurological sequelae that, and otherwise produces similar clinical symptoms as other co-circulating pathogens. Past infection with one member of the Flavivirus genus often induces cross-reactive antibodies against other flaviruses. These attributes complicate the ability to differentially diagnose ZIKV infection from other endemic mosquito-borne viruses, making it both a public health issue as well as a diagnostic challenge. We report the results from serological analyses using arbovirus-specific peptides on 339 samples that were previously collected from 6 countries. Overall, we found that our multiplexed peptide-based ELISA was highly efficient for identifying ZIKV antibodies as early as 2 weeks post infection, and that it correlates with microneutralization, plaque reduction neutralization tests (PRNTs) and commercial tests for ZIKV in previously characterized samples. We observed that seropositivity varied by patient cohort, reflecting the sampling period in relation to the 2015\u20132016 ZIKV outbreak. This work evaluates the accuracy, specificity, and sensitivity of our peptide-based ELISA method for detecting ZIKV antibodies from geographically diverse regions. These findings can contribute to ongoing serological methods development and can be adapted for use in future studies.<\/jats:p>","DOI":"10.3390\/v13071319","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T10:42:17Z","timestamp":1625740937000},"page":"1319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of ELISA-Based Multiplex Peptides for the Detection of Human Serum Antibodies Induced by Zika Virus Infection across Various Countries"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8885-5008","authenticated-orcid":false,"given":"Maria del Pilar","family":"Martinez Viedma","sequence":"first","affiliation":[{"name":"J. Craig Venter Institute, La Jolla, CA 92137, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Panossian","sequence":"additional","affiliation":[{"name":"J. Craig Venter Institute, Rockville, MD 20850, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kennedy","family":"Gifford","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigacion en Microbiologia, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa, Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isis","family":"Figueroa","sequence":"additional","affiliation":[{"name":"Instituto de Investigacion en Microbiologia, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa, Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leda","family":"Parham","sequence":"additional","affiliation":[{"name":"Instituto de Investigacion en Microbiologia, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa, Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-5860","authenticated-orcid":false,"given":"Laise","family":"de Moraes","sequence":"additional","affiliation":[{"name":"Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lillian","family":"Nunes Gomes","sequence":"additional","affiliation":[{"name":"Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Garc\u00eda-Salum","sequence":"additional","affiliation":[{"name":"Departmento de Enfermedades Infecciosas e Inmunolog\u00eda Pedi\u00e1trica, Escuela de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Santiago H955+8Q, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Perret","sequence":"additional","affiliation":[{"name":"Departmento de Enfermedades Infecciosas e Inmunolog\u00eda Pedi\u00e1trica, Escuela de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Santiago H955+8Q, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Weiskopf","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gene S.","family":"Tan","sequence":"additional","affiliation":[{"name":"J. Craig Venter Institute, La Jolla, CA 92137, USA"},{"name":"Department of Medicine, Infectious Diseases Division, University of California San Diego, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio","family":"Augusto Silva","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio-Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds 65000-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7241-6844","authenticated-orcid":false,"given":"Viviane","family":"Boaventura","sequence":"additional","affiliation":[{"name":"Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil"},{"name":"Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo M.","family":"Ruiz-Palacios","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Nutricion, Universidad Nacional Autonoma de Mexico, Mexico City 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA"},{"name":"Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aruna Dharshan","family":"De Silva","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA"},{"name":"Genetech Research Institute, Colombo 00800, Sri Lanka"},{"name":"Department of Paraclinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Ratmalana 10390, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5013-6884","authenticated-orcid":false,"given":"Rafael A.","family":"Medina","sequence":"additional","affiliation":[{"name":"Departmento de Enfermedades Infecciosas e Inmunolog\u00eda Pedi\u00e1trica, Escuela de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Santiago H955+8Q, Chile"},{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivette","family":"Lorenzana","sequence":"additional","affiliation":[{"name":"Instituto de Investigacion en Microbiologia, Universidad Nacional Aut\u00f3noma de Honduras, Tegucigalpa, Honduras"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevan M.","family":"Akrami","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil"},{"name":"Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5664-4436","authenticated-orcid":false,"given":"Ricardo","family":"Khouri","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Infectious Diseases, University of Colorado School of Medicine, Aurora, CO 80045, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7930-8160","authenticated-orcid":false,"given":"Brett E.","family":"Pickett","sequence":"additional","affiliation":[{"name":"J. 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Dis., 11.","DOI":"10.1371\/journal.pntd.0005990"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/cid\/ciw211","article-title":"Sexual Transmission of Zika Virus: Implications for Clinical Care and Public Health Policy","volume":"63","author":"Moreira","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1001.e1","DOI":"10.1016\/j.cmi.2017.07.006","article-title":"Recipient Epidemiology and Donor Evaluation Study (REDS-III) ZIKV Study Group. Detection of Zika Virus RNA in Semen of Asymptomatic Blood Donors","volume":"23","author":"Musso","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika Virus Outbreak on Yap Island, Federated States of Micronesia","volume":"360","author":"Duffy","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_6","first-page":"329","article-title":"Zika Virus: A New Epidemic on our Doorstep","volume":"67","year":"2015","journal-title":"Rev. Investig. Clin."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1002\/ana.24748","article-title":"Zika Virus: An Emergent Neuropathological Agent","volume":"80","author":"White","year":"2016","journal-title":"Ann. Neurol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.3201\/eid1509.090442","article-title":"Zika Virus Outside Africa","volume":"15","author":"Hayes","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007","volume":"14","author":"Lanciotti","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.chom.2010.08.007","article-title":"The Human Immune Response to Dengue Virus is Dominated by Highly Cross-Reactive Antibodies Endowed with Neutralizing and Enhancing Activity","volume":"8","author":"Beltramello","year":"2010","journal-title":"Cell. Host Microbe"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Deng, Y.Q., Dai, J.X., Ji, G.H., Jiang, T., Wang, H.J., Yang, H.O., Tan, W.L., Liu, R., Yu, M., and Ge, B.X. (2011). A Broadly Flavivirus Cross-Neutralizing Monoclonal Antibody that Recognizes a Novel Epitope within the Fusion Loop of E Protein. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0016059"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"9557","DOI":"10.1128\/JVI.00080-06","article-title":"Cryptic Properties of a Cluster of Dominant Flavivirus Cross-Reactive Antigenic Sites","volume":"80","author":"Stiasny","year":"2006","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.aaf8505","article-title":"Specificity, Cross-Reactivity, and Function of Antibodies Elicited by Zika Virus Infection","volume":"353","author":"Stettler","year":"2016","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7852","DOI":"10.1073\/pnas.1607931113","article-title":"Human Antibody Responses after Dengue Virus Infection are Highly Cross-Reactive to Zika Virus","volume":"113","author":"Priyamvada","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/emmm.201200213","article-title":"Early Neutralizing IgG Response to Chikungunya Virus in Infected Patients Targets a Dominant Linear Epitope on the E2 Glycoprotein","volume":"4","author":"Kam","year":"2012","journal-title":"EMBO Mol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kam, Y.W., Pok, K.Y., Eng, K.E., Tan, L.K., Kaur, S., Lee, W.W., Leo, Y.S., Ng, L.C., and Ng, L.F. (2015). Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003445"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.4269\/ajtmh.18-0756","article-title":"Neutralizing Antibodies from Convalescent Chikungunya Virus Patients can Cross-Neutralize Mayaro and Una Viruses","volume":"100","author":"Martins","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.apjtm.2016.01.017","article-title":"Immunological Evidence of Zika Virus Transmission in Thailand","volume":"9","author":"Wikan","year":"2016","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e01785-17","DOI":"10.1128\/JCM.01785-17","article-title":"Comparison of Four Serological Methods and Two Reverse Transcription-PCR Assays for Diagnosis and Surveillance of Zika Virus Infection","volume":"56","author":"Balmaseda","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00095-18","DOI":"10.1128\/mBio.00095-18","article-title":"Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay","volume":"9","author":"Mishra","year":"2018","journal-title":"MBio"},{"key":"ref_21","unstructured":"Bosch, I., de Puig, H., Hiley, M., Carre-Camps, M., Perdomo-Celis, F., Narvaez, C.F., Salgado, D.M., Senthoor, D., O\u2019Grady, M., and Phillips, E. (2017). Rapid Antigen Tests for Dengue Virus Serotypes and Zika Virus in Patient Serum. Sci. Transl. Med., 9."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jviromet.2018.03.007","article-title":"Development of an Anti-Dengue NS1 IgG ELISA to Evaluate Exposure to Dengue Virus","volume":"257","author":"Nascimento","year":"2018","journal-title":"J. Virol. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Lee, A.J., Bhattacharya, R., Scheuermann, R.H., and Pickett, B.E. (2017). Identification of Diagnostic Peptide Regions that Distinguish Zika Virus from Related Mosquito-Borne Flaviviruses. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0178199"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.12688\/f1000research.20981.1","article-title":"Peptide Arrays Incubated with Three Collections of Human Sera from Patients Infected with Mosquito-Borne Viruses","volume":"8","author":"Kose","year":"2019","journal-title":"F1000Research"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1128\/JCM.00844-09","article-title":"Use of the Normalized Absorbance Ratio as an Internal Standardization Approach to Minimize Measurement Error in Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Papillomavirus Infection","volume":"48","author":"Ramanakumar","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","first-page":"e52065","article-title":"Viral Concentration Determination through Plaque Assays: Using Traditional and Novel Overlay Systems","volume":"93","author":"Baer","year":"2014","journal-title":"J. Vis. Exp."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"708","DOI":"10.4269\/ajtmh.19-0270","article-title":"Use of a Blockade-of-Binding ELISA and Microneutralization Assay to Evaluate Zika Virus Serostatus in Dengue-Endemic Areas","volume":"101","author":"Nascimento","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sabalza, M., Barber, C.A., Abrams, W.R., Montagna, R., and Malamud, D. (2017). Zika Virus Specific Diagnostic Epitope Discovery. J. Vis. Exp., 130.","DOI":"10.3791\/56784"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hoen, B., Carpentier, M., Gaete, S., Tressieres, B., Herrmann-Storck, C., Vingadassalom, I., Huc-Anais, P., Funk, A.L., Fontanet, A., and de Lamballerie, X. (2019). Kinetics of Anti-Zika Virus Antibodies After Acute Infection in Pregnant Women. J. Clin. Microbiol., 57.","DOI":"10.1128\/JCM.01151-19"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.jns.2017.02.029","article-title":"Probable Zika Virus-Associated Guillain-Barre Syndrome: Challenges with Clinico-Laboratory Diagnosis","volume":"375","author":"Miller","year":"2017","journal-title":"J. Neurol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ebiom.2017.03.006","article-title":"A Rapid Zika Diagnostic Assay to Measure Neutralizing Antibodies in Patients","volume":"17","author":"Shan","year":"2017","journal-title":"EBioMedicine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8384","DOI":"10.1073\/pnas.1704984114","article-title":"Antibody-Based Assay Discriminates Zika Virus Infection from other Flaviviruses","volume":"114","author":"Balmaseda","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.bjid.2017.03.018","article-title":"Low Seroprevalence of Zika Virus in Cameroonian Blood Donors","volume":"21","author":"Gake","year":"2017","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1182\/blood-2016-09-737981","article-title":"Zika Virus in Asymptomatic Blood Donors in Martinique","volume":"129","author":"Gallian","year":"2017","journal-title":"Blood"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Saba Villarroel, P.M., Nurtop, E., Pastorino, B., Roca, Y., Drexler, J.F., Gallian, P., Jaenisch, T., Leparc-Goffart, I., Priet, S., and Ninove, L. (2018). Zika Virus Epidemiology in Bolivia: A Seroprevalence Study in Volunteer Blood Donors. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006239"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/JCM.00442-17","article-title":"Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing","volume":"55","author":"Kristjanson","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Dhar-Chowdhury, P., Paul, K.K., Haque, C.E., Hossain, S., Lindsay, L.R., Dibernardo, A., Brooks, W.A., and Drebot, M.A. (2017). Dengue Seroprevalence, Seroconversion and Risk Factors in Dhaka, Bangladesh. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005475"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Petitdemange, C., Becquart, P., Wauquier, N., Beziat, V., Debre, P., Leroy, E.M., and Vieillard, V. (2011). Unconventional Repertoire Profile is Imprinted during Acute Chikungunya Infection for Natural Killer Cells Polarization toward Cytotoxicity. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002268"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/7\/1319\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:27:47Z","timestamp":1760164067000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/7\/1319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,8]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["v13071319"],"URL":"https:\/\/doi.org\/10.3390\/v13071319","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2021,7,8]]}}}