{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T09:06:57Z","timestamp":1769245617803,"version":"3.49.0"},"reference-count":76,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Indian Council of Medical Research","award":["5\/8-I(12)\/2020-ECD-II"],"award-info":[{"award-number":["5\/8-I(12)\/2020-ECD-II"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Viral infections lead to expeditious activation of the host\u2019s innate immune responses, most importantly the interferon (IFN) response, which manifests a network of interferon-stimulated genes (ISGs) that constrain escalating virus replication by fashioning an ill-disposed environment. Interestingly, most viruses, including rotavirus, have evolved numerous strategies to evade or subvert host immune responses to establish successful infection. Several studies have documented the induction of ISGs during rotavirus infection. In this study, we evaluated the induction and antiviral potential of viperin, an ISG, during rotavirus infection. We observed that rotavirus infection, in a stain independent manner, resulted in progressive upregulation of viperin at increasing time points post-infection. Knockdown of viperin had no significant consequence on the production of total infectious virus particles. Interestingly, substantial escalation in progeny virus release was observed upon viperin knockdown, suggesting the antagonistic role of viperin in rotavirus release. Subsequent studies unveiled that RV-NSP4 triggered relocalization of viperin from the ER, the normal residence of viperin, to mitochondria during infection. Furthermore, mitochondrial translocation of NSP4 was found to be impeded by viperin, leading to abridged cytosolic release of Cyt c and subsequent inhibition of intrinsic apoptosis. Additionally, co-immunoprecipitation studies revealed that viperin associated with NSP4 through regions including both its radical SAM domain and its C-terminal domain. Collectively, the present study demonstrated the role of viperin in restricting rotavirus egress from infected host cells by modulating NSP4 mediated apoptosis, highlighting a novel mechanism behind viperin\u2019s antiviral action in addition to the intricacy of viperin\u2013virus interaction.<\/jats:p>","DOI":"10.3390\/v13071324","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T10:42:17Z","timestamp":1625740937000},"page":"1324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Viperin, an IFN-Stimulated Protein, Delays Rotavirus Release by Inhibiting Non-Structural Protein 4 (NSP4)-Induced Intrinsic Apoptosis"],"prefix":"10.3390","volume":"13","author":[{"given":"Rakesh","family":"Sarkar","sequence":"first","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satabdi","family":"Nandi","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahadeb","family":"Lo","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Animesh","family":"Gope","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamta","family":"Chawla-Sarkar","sequence":"additional","affiliation":[{"name":"Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road Scheme-XM, Beliaghata, Kolkata 700010, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,8]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Rotavirus infection","volume":"3","author":"Crawford","year":"2017","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_2","first-page":"118","article-title":"Three-dimensional visualization of mRNA release from actively transcribing rotavirus particles","volume":"4","author":"Lawton","year":"1997","journal-title":"Nat. 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