{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:11:52Z","timestamp":1760235112791,"version":"build-2065373602"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T00:00:00Z","timestamp":1626307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This research was partly funded by a Project under Her Royal Highness Princess Chulabhorn Mahidol through the \u201cSaving Animals and Human Lives from Rabies\u201d Project. Travel expenses of the first author were funded by Ceva Innovation Center GmbH.","award":["Not applicable."],"award-info":[{"award-number":["Not applicable."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Oral vaccination of dogs against rabies has the potential to achieve mass coverage and thus deplete the virus of its most important reservoir host species. There is, however, no established non-invasive method to evaluate vaccine release in the oral cavity, following bait ingestion. In this study, two pre-selected marker methods in conjunction with their acceptance were assessed in local Thai dogs. Shelter dogs (n = 47) were offered one of four randomized bait formulations; bait type A-, containing Green S (E142) in a fructose solution; type B-, containing Patent Blue V (E131) in a fructose solution; type C-, containing the medium used for delivery of oral rabies vaccine in baits commercially produced; and type D-, containing denatonium benzoate, which was to serve as the negative control, due to its perceived bitterness. Patent Blue V was found to possess overall stronger dyeing capacities compared to Green S. Furthermore, there was no significant difference in the acceptance or bait handling of Patent Blue V baits compared to those containing the oral rabies vaccine medium alone, suggesting the potential use of this dye as a surrogate for rabies vaccine when testing newly developed bait formats.<\/jats:p>","DOI":"10.3390\/v13071382","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T23:28:30Z","timestamp":1626391710000},"page":"1382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Study of Optical Markers to Assess Bait System Efficiency Concerning Vaccine Release in the Oral Cavity of Dogs"],"prefix":"10.3390","volume":"13","author":[{"given":"Anna","family":"Langguth","sequence":"first","affiliation":[{"name":"University of Veterinary Medicine Hannover, Foundation, B\u00fcnteweg 2, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6101-5191","authenticated-orcid":false,"given":"Kansuda","family":"Leelahapongsathon","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Napasaporn","family":"Wannapong","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-6514","authenticated-orcid":false,"given":"Suwicha","family":"Kasemsuwan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Ortmann","sequence":"additional","affiliation":[{"name":"Ceva Innovation Center GmbH, Am Pharmapark, 06830 Dessau-Rosslau, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ad","family":"Vos","sequence":"additional","affiliation":[{"name":"Ceva Innovation Center GmbH, Am Pharmapark, 06830 Dessau-Rosslau, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"B\u00f6er","sequence":"additional","affiliation":[{"name":"Department of Ethology, University of Osnabr\u00fcck, Barbarastra\u00dfe 11, 49076 Osnabr\u00fcck, Germany"},{"name":"Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Foundation, B\u00fcnteweg 2, 30559 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,15]]},"reference":[{"key":"ref_1","first-page":"765","article-title":"M\u00e9thode pour pr\u00e9venir la rage apr\u00e8s morsure","volume":"101","author":"Pasteur","year":"1885","journal-title":"C. 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