{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:12:40Z","timestamp":1760235160391,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,24]],"date-time":"2021-07-24T00:00:00Z","timestamp":1627084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Agency of Medical Research and Development (AMED)","award":["JP21wm0125006, JP21wm0225017"],"award-info":[{"award-number":["JP21wm0125006, JP21wm0225017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue virus (DENV) is one of the most prevalent neglected tropical diseases, with half of the world\u2019s population at risk of infection. In Nepal, DENV was first reported in 2004, and its prevalence is increasing every year. The present study aimed to obtain and characterize the full-length genome sequence of DENV from the 2017 outbreak. Hospital-based surveillance was conducted in two provinces of Nepal during the outbreak. Acute-phase serum samples were collected from 141 clinically suspected dengue patients after the rainy season. By serological and molecular techniques, 37 (26.9%) and 49 (34.8%), respectively, were confirmed as dengue patients. The cosmopolitan genotype of DENV-2 was isolated from 27 laboratory-confirmed dengue patients. Genomic analysis showed many amino acid substitutions distributed mainly among the E, NS3, and NS5 genes. Phylogenetic analyses of the whole genome sequence revealed two clades (Asian and Indian) among DENV-2 isolates from Nepal. The DENV isolates from hilly and Terai areas were similar to Asian and Indian strains, respectively. Further genomic study on different DENV serotypes is warranted to understand DENV epidemics in Nepal, where there are limited scientific resources and infrastructure.<\/jats:p>","DOI":"10.3390\/v13081444","type":"journal-article","created":{"date-parts":[[2021,7,25]],"date-time":"2021-07-25T22:07:00Z","timestamp":1627250820000},"page":"1444","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["An Outbreak of Dengue Virus Serotype 2 Cosmopolitan Genotype in Nepal, 2017"],"prefix":"10.3390","volume":"13","author":[{"given":"Mya Myat","family":"Ngwe Tun","sequence":"first","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0619-9108","authenticated-orcid":false,"given":"Kishor","family":"Pandey","sequence":"additional","affiliation":[{"name":"Central Department of Zoology, Institute of Science and Technology, Tribhuvan University, Kathmandu 44060, Nepal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Nabeshima","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-8030","authenticated-orcid":false,"given":"Aung Kyaw","family":"Kyaw","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandira","family":"Adhikari","sequence":"additional","affiliation":[{"name":"Shi-Gan International College of Science and Technology, Kathmandu 44060, Nepal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra Kendra","family":"Raini","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4072-0745","authenticated-orcid":false,"given":"Shyam Prakash","family":"Dumre","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Institute of Science and Technology, Tribhuvan University, Kirtipur 44618, Nepal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basu Dev","family":"Pandey","sequence":"additional","affiliation":[{"name":"Ministry of Health and Population, Kathmandu 44060, Nepal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouichi","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,24]]},"reference":[{"key":"ref_1","unstructured":"WHO (1997). 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