{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:22:58Z","timestamp":1778631778902,"version":"3.51.4"},"reference-count":85,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T00:00:00Z","timestamp":1627344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>A vaccine against congenital cytomegalovirus infection is a high priority. Guinea pig cytomegalovirus (GPCMV) is the only congenital CMV small animal model. GPCMV encodes essential glycoprotein complexes for virus entry (gB, gH\/gL\/gO, gM\/gN) including a pentamer complex (gH\/gL\/GP129\/GP131\/GP133 or PC) for endocytic cell entry. The cohorts for protection against congenital CMV are poorly defined. Neutralizing antibodies to the viral glycoprotein complexes are potentially more important than an immunodominant T-cell response to the pp65 protein. In GPCMV, GP83 (pp65 homolog) is an evasion factor, and the GP83 mutant GPCMV has increased sensitivity to type I interferon. Although GP83 induces a cell-mediated response, a GP83-only-based vaccine strategy has limited efficacy. GPCMV attenuation via GP83 null deletion mutant in glycoprotein PC positive or negative virus was evaluated as live-attenuated vaccine strains (GP83dPC+\/PC-). Vaccinated animals induced antibodies to viral glycoprotein complexes, and PC+ vaccinated animals had sterilizing immunity against wtGPCMV challenge. In a pre-conception vaccine (GP83dPC+) study, dams challenged mid-2nd trimester with wtGPCMV had complete protection against congenital CMV infection without detectable virus in pups. An unvaccinated control group had 80% pup transmission rate. Overall, gB and PC antibodies are key for protection against congenital CMV infection, but a response to pp65 is not strictly necessary.<\/jats:p>","DOI":"10.3390\/v13081467","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T22:35:02Z","timestamp":1627425302000},"page":"1467","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Fully Protective Congenital CMV Vaccine Requires Neutralizing Antibodies to Viral Pentamer and gB Glycoprotein Complexes but a pp65 T-Cell Response Is Not Necessary"],"prefix":"10.3390","volume":"13","author":[{"given":"K. Yeon","family":"Choi","sequence":"first","affiliation":[{"name":"Health Science Center, Department of Microbial Pathogenesis & Immunology, College of Medicine, Texas A&M University, Bryan, TX 77807-3260, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alistair","family":"McGregor","sequence":"additional","affiliation":[{"name":"Health Science Center, Department of Microbial Pathogenesis & Immunology, College of Medicine, Texas A&M University, Bryan, TX 77807-3260, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,27]]},"reference":[{"key":"ref_1","first-page":"240","article-title":"Immunization strategy for prevention of congenital cytomegalovirus infection","volume":"5","author":"Pass","year":"1996","journal-title":"Infect Agents Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1053\/j.spid.2004.09.011","article-title":"Congenital cytomegalovirus infection: Outcome and diagnosis","volume":"16","author":"Ross","year":"2005","journal-title":"Semin. Pediatr. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/rmv.544","article-title":"New estimates of the prevalence of neurological and sensory sequelae and mortality associated with congenital cytomegalovirus infection","volume":"17","author":"Dollard","year":"2007","journal-title":"Rev. Med. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/CMR.00062-12","article-title":"The \u201csilent\u201d global burden of congenital cytomegalovirus","volume":"26","author":"Manicklal","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1002\/rmv.1790","article-title":"Universal newborn screening for congenital CMV infection: What is the evidence of potential benefit?","volume":"24","author":"Cannon","year":"2014","journal-title":"Rev. Med. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1056\/NEJMra050700","article-title":"Newborn hearing screening\u2014A silent revolution","volume":"354","author":"Morton","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_7","unstructured":"Stratton, K.R., Durch, J.S., and Lawrence, R.S. (2000). Vaccines for the 21st Century: A Tool for Decisionmaking, National Academies Press. The National Academies Collection Report."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Britt, W.J. (2017). Congenital Human Cytomegalovirus Infection and the Enigma of Maternal Immunity. J. Virol., 91.","DOI":"10.1128\/JVI.02392-16"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1093\/infdis\/120.4.445","article-title":"Mouse Cytomegalovirus: Placental Infection","volume":"120","author":"Johnson","year":"1969","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1128\/jvi.55.2.402-409.1985","article-title":"The placenta as a site of cytomegalovirus infection in guinea pigs","volume":"55","author":"Griffith","year":"1985","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1093\/infdis\/138.5.650","article-title":"Experimental Congenital Infection with Cytomegalovirus: A Guinea Pig Model","volume":"138","author":"Kumar","year":"1978","journal-title":"J. Infect. Dis."},{"key":"ref_12","first-page":"32","article-title":"Guinea pig model of congenital CMV-induced hearing loss: A review","volume":"23","author":"Woolf","year":"1991","journal-title":"Transplant. Proc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1517\/17425255.2011.613824","article-title":"Cytomegalovirus antivirals and development of improved animal models","volume":"7","author":"McGregor","year":"2011","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.antiviral.2006.05.018","article-title":"Pivotal role of animal models in the development of new therapies for cytomegalovirus infections","volume":"71","author":"Kern","year":"2006","journal-title":"Antivir. Res."},{"key":"ref_15","unstructured":"Reddehase, M.J. (2013). The Guinea Pig Model of Congenital Cytomegalovirus Infection. Cytomegaloviruses: From Molecular Pathogenesis to Intervention, Caister Academic Press. [3rd ed.]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1159\/000150510","article-title":"Human Cytomegalovirus Glycoproteins","volume":"39","author":"Britt","year":"1996","journal-title":"Intervirology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1128\/jvi.62.6.1956-1962.1988","article-title":"A multigene family encodes the human cytomegalovirus glycoprotein complex gcII (gp 47\u201352 complex)","volume":"62","author":"Gretch","year":"1988","journal-title":"J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1128\/jvi.62.3.875-881.1988","article-title":"Identification and characterization of three distinct families of glycoprotein complexes in the envelopes of human cytomegalovirus","volume":"62","author":"Rasmussen","year":"1988","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"8191","DOI":"10.1128\/JVI.72.10.8191-8197.1998","article-title":"The human cytomegalovirus UL74 gene encodes the third component of the glycoprotein H-glycoprotein L-containing envelope complex","volume":"72","author":"Huber","year":"1998","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1056\/NEJMoa0804749","article-title":"Vaccine prevention of maternal cytomegalovirus infection","volume":"360","author":"Pass","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.1128\/JVI.80.9.4591-4600.2006","article-title":"Human cytomegalovirus infection elicits a glycoprotein M (gM)\/gN-specific virus-neutralizing antibody response","volume":"80","author":"Shimamura","year":"2006","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1016\/j.vaccine.2007.01.011","article-title":"DNA vaccines expressing glycoprotein complex II antigens gM and gN elicited neutralizing antibodies against multiple human cytomegalovirus (HCMV) isolates","volume":"25","author":"Shen","year":"2007","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Nguyen, C.C., and Kamil, J.P. (2018). Pathogen at the Gates: Human Cytomegalovirus Entry and Cell Tropism. Viruses, 10.","DOI":"10.20944\/preprints201811.0378.v2"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Coleman, S., Hornig, J., Maddux, S., Choi, K.Y., and McGregor, A. (2015). Viral Glycoprotein Complex Formation, Essential Function and Immunogenicity in the Guinea Pig Model for Cytomegalovirus. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137942"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Coleman, S., Choi, K.Y., Root, M., and McGregor, A. (2016). A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005755"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7902","DOI":"10.1128\/JVI.00283-16","article-title":"A Novel Non-Replication-Competent Cytomegalovirus Capsid Mutant Vaccine Strategy Is Effective in Reducing Congenital Infection","volume":"90","author":"Choi","year":"2016","journal-title":"J. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1099\/jgv.0.001394","article-title":"Requirements for guinea pig cytomegalovirus tropism and antibody neutralization on placental amniotic sac cells","volume":"101","author":"Choi","year":"2020","journal-title":"J. Gen. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1016\/j.vaccine.2020.01.063","article-title":"Neutralizing antibodies to gB based CMV vaccine requires full length antigen but reduced virus neutralization on non-fibroblast cells limits vaccine efficacy in the guinea pig model","volume":"38","author":"Choi","year":"2020","journal-title":"Vaccine"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10841","DOI":"10.1128\/JVI.76.21.10841-10848.2002","article-title":"The Genes Encoding the gCIII Complex of Human Cytomegalovirus Exist in Highly Diverse Combinations in Clinical Isolates","volume":"76","author":"Rasmussen","year":"2002","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Choi, K.Y., El-Hamdi, N.S., and McGregor, A. (2020). Convalescent Immunity to Guinea Pig Cytomegalovirus Induces Limited Cross Strain Protection against Re-Infection but High-Level Protection against Congenital Disease. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21175997"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1002\/rmv.645","article-title":"Human cytomegalovirus tropism for endothelial\/epithelial cells: Scientific background and clinical implications","volume":"20","author":"Revello","year":"2010","journal-title":"Rev. Med. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1099\/vir.0.064527-0","article-title":"Guinea pig cytomegalovirus GP129\/131\/133, homologues of human cytomegalovirus UL128\/130\/131A, are necessary for infection of monocytes and macrophages","volume":"95","author":"Yamada","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.virol.2017.06.008","article-title":"Cytomegalovirus UL128 homolog mutants that form a pentameric complex produce virus with impaired epithelial and trophoblast cell tropism and altered pathogenicity in the guinea pig","volume":"509","author":"Coleman","year":"2017","journal-title":"Virology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1099\/jgv.0.001137","article-title":"Differences in the effects of mutations in GP131, a guinea pig cytomegalovirus homologue of pentameric complex component UL130, on macrophage and epithelial cell infection","volume":"99","author":"Miura","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_35","first-page":"1","article-title":"Platelet-derived growth factor-\u03b1 receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer","volume":"1","author":"Kabanova","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Choi, K.Y., El-Hamdi, N.S., and McGregor, A. (2019). Inclusion of the Viral Pentamer Complex in a Vaccine Design Greatly Improves Protection against Congenital Cytomegalovirus in the Guinea Pig Model. J. Virol., 93.","DOI":"10.1128\/JVI.01442-19"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.virol.2020.05.012","article-title":"Guinea pig cytomegalovirus trimer complex gH\/gL\/gO uses PDGFRA as universal receptor for cell fusion and entry","volume":"548","author":"Choi","year":"2020","journal-title":"Virology"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wu, Y., Prager, A., Boos, S., Resch, M., Brizi\u0107, I., Mach, M., Wildner, S., Scrivano, L., and Adler, B. (2017). Human cytomegalovirus glycoprotein complex gH\/gL\/gO uses PDGFR-\u03b1 as a key for entry. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006281"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/j.cell.2018.06.028","article-title":"An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor","volume":"174","author":"Marcandalli","year":"2018","journal-title":"Cell"},{"key":"ref_40","first-page":"7043","article-title":"Faculty Opinions recommendation of OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism","volume":"116","author":"Mori","year":"2019","journal-title":"Fac. Opin. Post Publ. Peer Rev. Biomed. Lit."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Vanarsdall, A.L., Pritchard, S.R., Wisner, T.W., Liu, J., Jardetzky, T.S., and Johnson, D.C. (2018). CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells. mBio, 9.","DOI":"10.1128\/mBio.00781-18"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-10587-1","article-title":"CD46 facilitates entry and dissemination of human cytomegalovirus","volume":"10","author":"Stein","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"15470","DOI":"10.1073\/pnas.0406821101","article-title":"Cellular integrins function as entry receptors for human cytomegalovirus via a highly conserved disintegrin-like domain","volume":"101","author":"Feire","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1053\/j.semperi.2007.01.002","article-title":"Recent Advances in the Prevention and Treatment of Congenital Cytomegalovirus Infections","volume":"31","author":"Adler","year":"2007","journal-title":"Semin. Perinatol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1093\/infdis\/165.2.381","article-title":"Antibodies to Recombinant-Derived Glycoprotein B after Natural Human Cytomegalovirus Infection Correlate with Neutralizing Activity","volume":"165","author":"Marshall","year":"1992","journal-title":"J. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1056\/NEJM199203053261003","article-title":"The Outcome of Congenital Cytomegalovirus Infection in Relation to Maternal Antibody Status","volume":"326","author":"Fowler","year":"1992","journal-title":"N. Engl. J. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1086\/382751","article-title":"Protection against Congenital Cytomegalovirus Infection and Disease in Guinea Pigs, Conferred by a Purified Recombinant Glycoprotein B Vaccine","volume":"189","author":"Schleiss","year":"2004","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.vaccine.2015.11.056","article-title":"Safety and efficacy of a cytomegalovirus glycoprotein B (gB) vaccine in adolescent girls: A randomized clinical trial","volume":"34","author":"Bernstein","year":"2016","journal-title":"Vaccine"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/S0140-6736(11)60136-0","article-title":"Cytomegalovirus glycoprotein-B vaccine with MF59 adjuvant in transplant recipients: A phase 2 randomised placebo-controlled trial","volume":"377","author":"Griffiths","year":"2011","journal-title":"Lancet"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1093\/infdis\/172.5.1212","article-title":"Reduced congenital cytomegalovirus (CMV) infection after maternal immunization with a guinea pig CMV glycoprotein before gestational primary CMV infection in the guinea pig model","volume":"172","author":"Harrison","year":"1995","journal-title":"J. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1386-6532(02)00100-2","article-title":"Animal models of congenital cytomegalovirus infection: An overview of progress in the characterization of guinea pig cytomegalovirus (GPCMV)","volume":"25","author":"Schleiss","year":"2002","journal-title":"J. Clin. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5580","DOI":"10.1016\/j.vaccine.2018.07.056","article-title":"Novel trimeric human cytomegalovirus glycoprotein B elicits a high-titer neutralizing antibody response","volume":"36","author":"Cui","year":"2018","journal-title":"Vaccine"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1099\/jgv.0.001579","article-title":"A trimeric capable gB CMV vaccine provides limited protection against a highly cell associated and epithelial tropic strain of cytomegalovirus in guinea pigs","volume":"102","author":"Choi","year":"2021","journal-title":"J. Gen. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5760","DOI":"10.1016\/j.vaccine.2008.07.092","article-title":"Cytomegalovirus vaccines fail to induce epithelial entry neutralizing antibodies comparable to natural infection","volume":"26","author":"Cui","year":"2008","journal-title":"Vaccine"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"7444","DOI":"10.1128\/JVI.00467-12","article-title":"Antibodies against the gH\/gL\/UL128\/UL130\/UL131 complex comprise the majority of the anti-cytomegalovirus (anti-CMV) neutralizing antibody response in CMV hyperimmune globulin","volume":"86","author":"Fouts","year":"2012","journal-title":"J. Virol."},{"key":"ref_56","first-page":"1142","article-title":"Preemptive Therapy for Systemic and Pulmonary Human Cytomegalovirus Infection in Lung Transplant Recipients","volume":"9","author":"Gerna","year":"2009","journal-title":"Arab. Archaeol. Epigr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1084\/jem.20050882","article-title":"Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects","volume":"202","author":"Sylwester","year":"2005","journal-title":"J. Exp. Med."},{"key":"ref_58","unstructured":"Reddehase, M.J. (2013). Adaptive Cellular Immunity to Human Cytomegalovirus. Cytomegaloviruses: From Molecular Pathogenesis to Intervention, Caister Academic Press."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"9872","DOI":"10.1128\/JVI.78.18.9872-9889.2004","article-title":"Molecular, Biological, and In Vivo Characterization of the Guinea Pig Cytomegalovirus (CMV) Homologs of the Human CMV Matrix Proteins pp71 (UL82) and pp65 (UL83)","volume":"78","author":"McGregor","year":"2004","journal-title":"J. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.virol.2017.01.023","article-title":"The essential role of guinea pig cytomegalovirus (GPCMV) IE1 and IE2 homologs in viral replication and IE1-mediated ND10 targeting","volume":"504","author":"Hornig","year":"2017","journal-title":"Virology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.humimm.2004.02.012","article-title":"Cytomegalovirus (CMV)-Specific cellular immune responses","volume":"65","author":"Harari","year":"2004","journal-title":"Hum. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"19227","DOI":"10.1038\/srep19227","article-title":"Cytotoxic polyfunctionality maturation of cytomegalovirus-pp65-specific CD4 + and CD8 + T-cell responses in older adults positively correlates with response size","volume":"6","author":"Chiu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"9662","DOI":"10.1038\/s41598-020-66804-1","article-title":"Human cytomegalovirus pp65 peptide-induced autoantibodies cross-reacts with TAF9 protein and induces lupus-like autoimmunity in BALB\/c mice","volume":"10","author":"Hsieh","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5959","DOI":"10.1128\/jvi.69.10.5959-5968.1995","article-title":"The dominant phosphoprotein pp65 (UL83) of human cytomegalovirus is dispensable for growth in cell culture","volume":"69","author":"Schmolke","year":"1995","journal-title":"J. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Biolatti, M., Dell\u2019Oste, V., Pautasso, S., Gugliesi, F., von Einem, J., Krapp, C., Jakobsen, M.R., Borgogna, C., Gariglio, M., and De Andrea, M. (2018). Human Cytomegalovirus Tegument Protein pp65 (pUL83) Dampens Type I Interferon Production by Inactivating the DNA Sensor cGAS without Affecting STING. J. Virol., 92.","DOI":"10.1128\/JVI.01774-17"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"8238","DOI":"10.1128\/JVI.00923-16","article-title":"Regulatory Interaction between the Cellular Restriction Factor IFI16 and Viral pp65 (pUL83) Modulates Viral Gene Expression and IFI16 Protein Stability","volume":"90","author":"Biolatti","year":"2016","journal-title":"J. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1086\/511982","article-title":"Preconceptual Administration of an Alphavirus Replicon UL83 (pp65 Homolog) Vaccine Induces Humoral and Cellular Immunity and Improves Pregnancy Outcome in the Guinea Pig Model of Congenital Cytomegalovirus Infection","volume":"195","author":"Schleiss","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Choi, K.Y., El-Hamdi, N., Hornig, J., and McGregor, A. (2021). Guinea Pig Cytomegalovirus Protective T Cell Antigen GP83 Is a Functional pp65 Homolog for Innate Immune Evasion and Pentamer-Dependent Virus Tropism. J. Virol., 95.","DOI":"10.1128\/JVI.00324-21"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1016\/j.vaccine.2016.03.005","article-title":"Replication-defective lymphocytic choriomeningitis virus vectors expressing guinea pig cytomegalovirus gB and pp65 homologs are protective against congenital guinea pig cytomegalovirus infection","volume":"34","author":"Cardin","year":"2016","journal-title":"Vaccine"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"13645","DOI":"10.1073\/pnas.1511526112","article-title":"Maternal CD4+T cells protect against severe congenital cytomegalovirus disease in a novel nonhuman primate model of placental cytomegalovirus transmission","volume":"112","author":"Bialas","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2878","DOI":"10.1128\/JVI.00883-10","article-title":"Vaccine-Induced Control of Viral Shedding following Rhesus Cytomegalovirus Challenge in Rhesus Macaques","volume":"85","author":"Abel","year":"2010","journal-title":"J. Virol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1001\/jama.1973.03220330013003","article-title":"Immunologic properties of RA27-3 rubella virus vaccine. A comparison with strains presently licensed in the United States","volume":"225","author":"Plotkin","year":"1973","journal-title":"JAMA"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1016\/S0140-6736(74)90144-5","article-title":"Live Vaccine Used to Prevent the Spread of Varicella in Children in Hospital","volume":"304","author":"Takahashi","year":"1974","journal-title":"Lancet"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.2217\/fvl.13.107","article-title":"An attenuated CMV vaccine with a deletion in tegument protein GP83 (pp65 homolog) protects against placental infection and improves pregnancy outcome in a Guinea pig challenge model","volume":"8","author":"Schleiss","year":"2013","journal-title":"Future Virol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jviromet.2008.01.031","article-title":"Cloning the complete guinea pig cytomegalovirus genome as an infectious bacterial artificial chromosome with excisable origin of replication","volume":"149","author":"Cui","year":"2008","journal-title":"J. Virol. Methods"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1006\/mgme.2000.3102","article-title":"Molecular Cloning of the Guinea Pig Cytomegalovirus (GPCMV) Genome as an Infectious Bacterial Artificial Chromosome (BAC) in Escherichia coli","volume":"72","author":"McGregor","year":"2001","journal-title":"Mol. Genet. Metab."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.antiviral.2008.01.008","article-title":"Expression of the human cytomegalovirus UL97 gene in a chimeric guinea pig cytomegalovirus (GPCMV) results in viable virus with increased susceptibility to ganciclovir and maribavir","volume":"78","author":"McGregor","year":"2008","journal-title":"Antivir. Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.virol.2010.10.028","article-title":"Guinea pig cytomegalovirus GP84 is a functional homolog of the human cytomegalovirus (HCMV) UL84 gene that can complement for the loss of UL84 in a chimeric HCMV","volume":"410","author":"McGregor","year":"2011","journal-title":"J. Virol."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Vanarsdall, A.L., Howard, P.W., Wisner, T.W., and Johnson, D.C. (2016). Human Cytomegalovirus gH\/gL Forms a Stable Complex with the Fusion Protein gB in Virions. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005564"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"362ra145","DOI":"10.1126\/scitranslmed.aaf9387","article-title":"A replication-defective human cytomegalovirus vaccine for prevention of congenital infection","volume":"8","author":"Wang","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00430-008-0074-5","article-title":"Evaluation of recombinant modified vaccinia Ankara virus-based rhesus cytomegalovirus vaccines in rhesus macaques","volume":"197","author":"Yue","year":"2008","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1080\/21645515.2019.1593729","article-title":"Development of novel vaccines against human cytomegalovirus","volume":"15","author":"Cui","year":"2019","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.4049\/jimmunol.172.4.2256","article-title":"Human Cytomegalovirus Proteins pp65 and Immediate Early Protein 1 Are Common Targets for CD8+T Cell Responses in Children with Congenital or Postnatal Human Cytomegalovirus Infection","volume":"172","author":"Gibson","year":"2004","journal-title":"J. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1128\/JVI.02550-06","article-title":"Functional analysis of the murine cytomegalovirus chemokine receptor homologue M33: Ablation of constitutive signaling is associated with an attenuated phenotype in vivo","volume":"82","author":"Case","year":"2008","journal-title":"J. Virol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-55508-w","article-title":"Characterization of a live-attenuated HCMV-based vaccine platform","volume":"9","author":"Caposio","year":"2019","journal-title":"Sci. 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