{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:09:03Z","timestamp":1791947343712,"version":"4.3.4"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T00:00:00Z","timestamp":1629072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key Research and Development Program of China","award":["2018YFD0900501"],"award-info":[{"award-number":["2018YFD0900501"]}]},{"name":"Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)","award":["311021006"],"award-info":[{"award-number":["311021006"]}]},{"name":"the Key Research and Development Program of Zhuhai city","award":["2021"],"award-info":[{"award-number":["2021"]}]},{"name":"Guangdong Provincial Special Fund for MAITIT","award":["2019KJ141"],"award-info":[{"award-number":["2019KJ141"]}]},{"name":"the Science and Technology Planning Project of Guangdong Province","award":["2018A050506027"],"award-info":[{"award-number":["2018A050506027"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Scale drop disease virus (SDDV), an emerging piscine iridovirus prevalent in farmed Asian seabass Lates calcarifer in Southeast Asia, was firstly scientifically descripted in Singapore in 2015. Here, an SDDV isolate ZH-06\/20 was isolated by inoculating filtered ascites from diseased juvenile yellowfin seabream into MFF-1 cell. Advanced cytopathic effects were observed 6 days post-inoculation. A transmission electron microscopy examination confirmed that numerous virion particles, about 140 nm in diameter, were observed in infected MFF-1 cell. ZH-06\/20 was further purified and both whole genome and virion proteome were determined. The results showed that ZH-06\/20 was composed of 131,122 bp with 135 putative viral proteins and 113 of them were further detected by virion proteome. Western blot analysis showed that no (or weak) cross-reaction was observed among several major viral proteins between ZH-06\/20 and ISKNV-like megalocytivirus. An artificial challenge showed that ZH-06\/20 could cause 100% death to juvenile yellowfin seabream. A typical sign was characterized by severe ascites, but not scale drop, which was considerably different from SDD syndrome in Asian seabass. Collectively, SDDV was confirmed, for the first time, as the causative agent of ascites diseases in farmed yellowfin seabream. Our study offers useful information to better understanding SDDV-associated diseases in farmed fish.<\/jats:p>","DOI":"10.3390\/v13081617","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T21:28:04Z","timestamp":1629149284000},"page":"1617","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Scale Drop Disease Virus Associated Yellowfin Seabream (Acanthopagrus latus) Ascites Diseases, Zhuhai, Guangdong, Southern China: The First Description"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5604-4617","authenticated-orcid":false,"given":"Yuting","family":"Fu","sequence":"first","affiliation":[{"name":"School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6568-6857","authenticated-orcid":false,"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"Zhuhai Modern Agriculture Development Center, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixuan","family":"Fu","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huibing","family":"Su","sequence":"additional","affiliation":[{"name":"Zhuhai Modern Agriculture Development Center, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congling","family":"Huang","sequence":"additional","affiliation":[{"name":"Zhuhai Modern Agriculture Development Center, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoping","family":"Weng","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhao","family":"Yu","sequence":"additional","affiliation":[{"name":"Zhuhai Modern Agriculture Development Center, Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"He","sequence":"additional","affiliation":[{"name":"School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6276-7993","authenticated-orcid":false,"given":"Chuanfu","family":"Dong","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"},{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1099\/jgv.0.000818","article-title":"ICTV Virus Taxonomy Profile: Iridoviridae","volume":"98","author":"Chinchar","year":"2017","journal-title":"J. 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