{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:23:08Z","timestamp":1791328988500,"version":"4.3.1"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["UP8"],"award-info":[{"award-number":["UP8"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus, the bank vole (45%); Apodemus flavicollis, the yellow-necked mouse (29%); and Apodemus agrarius, the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus, A. glareolus, and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis, one Mus musculus, and one Sorex minutus. We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus, 1 M. musculus, and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92\u201395% nucleotide or 99\u2013100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine.<\/jats:p>","DOI":"10.3390\/v13081640","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T22:51:00Z","timestamp":1629327060000},"page":"1640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4506-115X","authenticated-orcid":false,"given":"Evan P.","family":"Williams","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Biochemistry, The University of Tennessee Health Science Center, Memphis, TN 38163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2378-9350","authenticated-orcid":false,"given":"Mariah K.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biochemistry, The University of Tennessee Health Science Center, Memphis, TN 38163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Demchyshyna","sequence":"additional","affiliation":[{"name":"Department of Virology, State Institution Public Health Center of the Ministry of Health of Ukraine, 04071 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Nebogatkin","sequence":"additional","affiliation":[{"name":"Public Health Center of the Ministry of Health of Ukraine, 01030 Kyiv, Ukraine"},{"name":"I.I. Schmalhausen Institute of Zoology of NAS of Ukraine, 01030 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olena","family":"Nesterova","sequence":"additional","affiliation":[{"name":"Black and Veatch Special Projects Corp., 01033 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Khuda","sequence":"additional","affiliation":[{"name":"Black and Veatch Special Projects Corp., 01033 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyudmyla","family":"Chernenko","sequence":"additional","affiliation":[{"name":"Department of Virology, State Institution Public Health Center of the Ministry of Health of Ukraine, 04071 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksandra A.","family":"Hluzd","sequence":"additional","affiliation":[{"name":"Department of Virology, State Institution Public Health Center of the Ministry of Health of Ukraine, 04071 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vira V.","family":"Kutseva","sequence":"additional","affiliation":[{"name":"Department of Virology, State Institution Public Health Center of the Ministry of Health of Ukraine, 04071 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory E.","family":"Glass","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute and Department of Geography, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliia","family":"Yanko","sequence":"additional","affiliation":[{"name":"Volyn Oblast Laboratory Center of the Ministry of Health of Ukraine, 43010 Lutsk, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2640-7672","authenticated-orcid":false,"given":"Colleen B.","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biochemistry, The University of Tennessee Health Science Center, Memphis, TN 38163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1007\/s00705-020-04752-x","article-title":"Changes to virus taxonomy and the Statutes ratified by the International Committee on Taxonomy of Viruses (2020)","volume":"165","author":"Walker","year":"2020","journal-title":"Arch. 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