{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T07:08:07Z","timestamp":1791443287717,"version":"4.3.3"},"reference-count":62,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["721367"],"award-info":[{"award-number":["721367"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The post-acute phase of SARS-CoV-2 infection was investigated in rhesus (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis). During the acute phase of infection, SARS-CoV-2 was shed via the nose and throat, and viral RNA was occasionally detected in feces. This phase coincided with a transient change in systemic immune activation. Even after the alleged resolution of the infection, computed tomography (CT) and positron emission tomography (PET)-CT revealed pulmonary lesions and activated tracheobronchial lymph nodes in all animals. Post-mortem histological examination of the lung tissue revealed mostly marginal or resolving minimal lesions that were indicative of SARS-CoV-2 infection. Evidence for SARS-CoV-2-induced histopathology was also found in extrapulmonary tissue samples, such as conjunctiva, cervical, and mesenteric lymph nodes. However, 5\u20136 weeks after SARS-CoV-2 exposure, upon necropsy, viral RNA was still detectable in a wide range of tissue samples in 50% of the macaques and included amongst others the heart, the respiratory tract and surrounding lymph nodes, salivary gland, and conjunctiva. Subgenomic messenger RNA was detected in the lungs and tracheobronchial lymph nodes, indicative of ongoing virus replication during the post-acute phase. These results could be relevant for understanding the long-term consequences of COVID-19 in humans.<\/jats:p>","DOI":"10.3390\/v13081673","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T23:19:33Z","timestamp":1629760773000},"page":"1673","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["The Post-Acute Phase of SARS-CoV-2 Infection in Two Macaque Species Is Associated with Signs of Ongoing Virus Replication and Pathology in Pulmonary and Extrapulmonary Tissues"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5330-2617","authenticated-orcid":false,"given":"Kinga P.","family":"B\u00f6sz\u00f6rm\u00e9nyi","sequence":"first","affiliation":[{"name":"Biomedical Primate Research Centre (BPRC), Department of Virology, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4261-6535","authenticated-orcid":false,"given":"Marieke A.","family":"Stammes","sequence":"additional","affiliation":[{"name":"Department of Parasitology, BPRC, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra C.","family":"Fagrouch","sequence":"additional","affiliation":[{"name":"Biomedical Primate Research Centre (BPRC), Department of Virology, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendoline","family":"Kiemenyi-Kayere","sequence":"additional","affiliation":[{"name":"Biomedical Primate Research Centre (BPRC), Department of Virology, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk","family":"Niphuis","sequence":"additional","affiliation":[{"name":"Biomedical Primate Research Centre (BPRC), Department of Virology, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella","family":"Mortier","sequence":"additional","affiliation":[{"name":"Biomedical Primate Research Centre (BPRC), Department of Virology, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3300-8204","authenticated-orcid":false,"given":"Nikki","family":"van Driel","sequence":"additional","affiliation":[{"name":"Animal Science Department, BPRC, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivonne","family":"Nieuwenhuis","sequence":"additional","affiliation":[{"name":"Department of Parasitology, BPRC, 2288 GJ Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A. 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Proteome Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1038\/s41379-020-0603-3","article-title":"A systematic review of pathological findings in COVID-19: A pathophysiological timeline and possible mechanisms of disease progression","volume":"33","author":"Polak","year":"2020","journal-title":"Mod. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1148\/rg.2020200159","article-title":"Chest CT in COVID-19: What the Radiologist Needs to Know","volume":"40","author":"Kwee","year":"2020","journal-title":"Radiographics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1080\/22221751.2020.1746199","article-title":"Hypothesis for potential pathogenesis of SARS-CoV-2 infection-a review of immune changes in patients with viral pneumonia","volume":"9","author":"Lin","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_5","first-page":"1","article-title":"A Review of Persistent Post-COVID Syndrome (PPCS)","volume":"3","author":"Oronsky","year":"2021","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_6","first-page":"1","article-title":"Long COVID hallmarks on [18F]FDG-PET\/CT: A case-control study","volume":"7","author":"Sollini","year":"2021","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/s41591-021-01292-y","article-title":"Attributes and predictors of long COVID","volume":"27","author":"Sudre","year":"2021","journal-title":"Nat. Med."},{"key":"ref_8","first-page":"259","article-title":"Post-acute COVID-19 syndrome","volume":"7862","author":"Nalbandian","year":"2021","journal-title":"Nat. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4851","DOI":"10.1111\/bph.15143","article-title":"Animal models of mechanisms of SARS-CoV-2 infection and COVID-19 pathology","volume":"177","author":"Cleary","year":"2020","journal-title":"Br. J. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1128\/JVI.02012-06","article-title":"Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus","volume":"81","author":"McCray","year":"2007","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40246-020-00272-6","article-title":"COVID-19 preclinical models: Human angiotensin-converting enzyme 2 transgenic mice","volume":"14","author":"Lutz","year":"2020","journal-title":"Hum. Genom."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/s41586-020-3035-9","article-title":"A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate","volume":"590","author":"Vercruysse","year":"2021","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5838","DOI":"10.1038\/s41467-020-19684-y","article-title":"STAT2 signaling restricts viral dissemination but drives severe pneumonia in SARS-CoV-2 infected hamsters","volume":"11","author":"Boudewijns","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.chom.2020.03.023","article-title":"Infection and Rapid Transmission of SARS-CoV-2 in Ferrets","volume":"27","author":"Kim","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3496","DOI":"10.1038\/s41467-020-17367-2","article-title":"SARS-CoV-2 is transmitted via contact and via the air between ferrets","volume":"11","author":"Richard","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/s41586-021-03361-1","article-title":"SARS-CoV-2 spike D614G change enhances replication and transmission","volume":"592","author":"Zhou","year":"2021","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1126\/science.abf4896","article-title":"Intranasal fusion inhibitory lipopeptide prevents direct-contact SARS-CoV-2 transmission in ferrets","volume":"371","author":"Schmitz","year":"2021","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"L379","DOI":"10.1152\/ajplung.00010.2008","article-title":"Animal models of acute lung injury","volume":"295","author":"Frevert","year":"2008","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/s41577-018-0005-7","article-title":"Nonhuman primate models of human viral infections","volume":"18","author":"Estes","year":"2018","journal-title":"Nat. Rev. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1002\/ame2.12017","article-title":"The battle against SARS and MERS coronaviruses: Reservoirs and Animal Models","volume":"1","author":"Gong","year":"2018","journal-title":"Anim. Model Exp. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.virol.2015.02.030","article-title":"Development of animal models against emerging coronaviruses: From SARS to MERS coronavirus","volume":"479\u2013480","author":"Sutton","year":"2015","journal-title":"Virology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41392-020-00269-6","article-title":"Comparison of nonhuman primates identified the suitable model for COVID-19","volume":"5","author":"Lu","year":"2020","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1007\/s12250-020-00252-z","article-title":"A Comprehensive Review of Animal Models for Coronaviruses: SARS-CoV-2, SARS-CoV, and MERS-CoV","volume":"35","author":"Singh","year":"2020","journal-title":"Virol. Sin."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Johnston, S.C., Ricks, K.M., Jay, A., Raymond, J.L., Rossi, F., Zeng, X., Scruggs, J., Dyer, D., Frick, O., and Koehler, J.W. (2021). Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0246366"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/s41590-020-00835-8","article-title":"Establishment of an African green monkey model for COVID-19 and protection against re-infection","volume":"22","author":"Woolsey","year":"2021","journal-title":"Nat. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/s41586-020-2324-7","article-title":"Respiratory disease in rhesus macaques inoculated with SARS-CoV-2","volume":"585","author":"Munster","year":"2020","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1126\/science.abb7314","article-title":"Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model","volume":"368","author":"Rockx","year":"2020","journal-title":"Science"},{"key":"ref_28","first-page":"389","article-title":"Thoracic radiography as a refinement methodology for the study of H1N1 influenza in cynomologus macaques (Macaca fascicularis)","volume":"60","author":"Brining","year":"2010","journal-title":"Comp. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2001483","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/s41586-020-2196-x","article-title":"Virological assessment of hospitalized patients with COVID-2019","volume":"581","author":"Wolfel","year":"2020","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Stammes, M.A., Bakker, J., Vervenne, R.A.W., Zijlmans, D.G.M., van Geest, L., Vierboom, M.P.M., Langermans, J.A.M., and Verreck, F.A.W. (2021). Recommendations for Standardizing Thorax PET\u2013CT in Non-Human Primates by Recent Experience from Macaque Studies. Animals, 11.","DOI":"10.3390\/ani11010204"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Finch, C.L., Crozier, I., Lee, J.H., Byrum, R., Cooper, T.K., Liang, J., Sharer, K., Solomon, J., Sayre, P.J., and Kocher, G. (2020). Characteristic and quantifiable COVID-19-like abnormalities in CT- and PET\/CT-imaged lungs of SARS-CoV-2-infected crab-eating macaques (Macaca fascicularis). bioRxiv.","DOI":"10.1101\/2020.05.14.096727"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1148\/radiol.2020200370","article-title":"Time Course of Lung Changes at Chest CT during Recovery from Coronavirus Disease 2019 (COVID-19)","volume":"295","author":"Pan","year":"2020","journal-title":"Radiology"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Maaskant, A., Meijer, L., Bakker, J., van Geest, L., Zijlmans, D.G.M., Langermans, J.A.M., Verschoor, E.J., and Stammes, M.A. (2021). Bronchoalveolar lavage affects thorax computed tomography of healthy and SARS-CoV-2 infected rhesus macaques bioRxiv. PloS ONE, 16.","DOI":"10.1101\/2021.02.04.429761"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115167","DOI":"10.1016\/j.diagmicrobio.2020.115167","article-title":"Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: A screening tool and a point-of-care test","volume":"98","author":"Hoste","year":"2020","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e02370-20","DOI":"10.1128\/JVI.02370-20","article-title":"Comparison of Subgenomic and Total RNA in SARS-CoV-2 Challenged Rhesus Macaques","volume":"95","author":"Dagotto","year":"2021","journal-title":"J. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.medmal.2020.03.007","article-title":"C-reactive protein levels in the early stage of COVID-19","volume":"50","author":"Wang","year":"2020","journal-title":"Med. Mal. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/s41581-020-0284-7","article-title":"Kidney involvement in COVID-19 and rationale for extracorporeal therapies","volume":"16","author":"Ronco","year":"2020","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/S2213-2600(20)30229-0","article-title":"Management of acute kidney injury in patients with COVID-19","volume":"8","author":"Ronco","year":"2020","journal-title":"Lancet Respir. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.kint.2020.03.005","article-title":"Kidney disease is associated with in-hospital death of patients with COVID-19","volume":"97","author":"Cheng","year":"2020","journal-title":"Kidney Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1055\/s-0040-1714350","article-title":"COVID-19-Related Severe Hypercoagulability in Patients Admitted to Intensive Care Unit for Acute Respiratory Failure","volume":"120","author":"Spiezia","year":"2020","journal-title":"Thromb. Haemost."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1182\/blood.2020006000","article-title":"COVID-19 and its implications for thrombosis and anticoagulation","volume":"135","author":"Connors","year":"2020","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2819","DOI":"10.1007\/s00330-020-07347-x","article-title":"The sensitivity and specificity of chest CT in the diagnosis of COVID-19","volume":"31","author":"Kovacs","year":"2021","journal-title":"Eur. Radiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2214\/ajr.144.2.261","article-title":"Normal mediastinal lymph nodes: Number and size according to American Thoracic Society mapping","volume":"144","author":"Glazer","year":"1985","journal-title":"AJR Am. J. Roentgenol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1152\/physiolgenomics.00087.2020","article-title":"SARS-CoV-2, ACE2 expression, and systemic organ invasion","volume":"53","author":"Ashraf","year":"2021","journal-title":"Physiol. Genom."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e887","DOI":"10.1097\/PR9.0000000000000887","article-title":"A clinical primer for the expected and potential post-COVID-19 syndromes","volume":"6","author":"Walitt","year":"2021","journal-title":"Pain Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1126\/science.abc4776","article-title":"SARS-CoV-2 infection protects against rechallenge in rhesus macaques","volume":"369","author":"Chandrashekar","year":"2020","journal-title":"Science"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41586-020-2423-5","article-title":"Clinical benefit of remdesivir in rhesus macaques infected with SARS-CoV-2","volume":"585","author":"Williamson","year":"2020","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"16598","DOI":"10.1073\/pnas.1310744110","article-title":"Middle East respiratory syndrome coronavirus (MERS-CoV) causes transient lower respiratory tract infection in rhesus macaques","volume":"110","author":"Rasmussen","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.cytogfr.2020.05.003","article-title":"The cytokine storm in COVID-19: An overview of the involvement of the chemokine\/chemokine-receptor system","volume":"53","author":"Coperchini","year":"2020","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0140-6736(20)30183-5","article-title":"Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China","volume":"395","author":"Huang","year":"2020","journal-title":"Lancet"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S0140-6736(20)30251-8","article-title":"Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding","volume":"395","author":"Lu","year":"2020","journal-title":"Lancet"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Hartman, A.L., Nambulli, S., McMillen, C.M., White, A.G., Tilston-Lunel, N.L., Albe, J.R., Cottle, E., Dunn, M.D., Frye, L.J., and Gilliland, T.H. (2020). SARS-CoV-2 infection of African green monkeys results in mild respiratory disease discernible by PET\/CT imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts. PLoS Pathog, 16.","DOI":"10.1101\/2020.06.20.137687"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1097\/RTI.0000000000000524","article-title":"Radiological Society of North America Expert Consensus Statement on Reporting Chest CT Findings Related to COVID-19. Endorsed by the Society of Thoracic Radiology, the American College of Radiology, and RSNA-Secondary Publication","volume":"35","author":"Simpson","year":"2020","journal-title":"J. Thorac. Imaging"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5214","DOI":"10.1007\/s00330-020-06898-3","article-title":"CT and COVID-19: Chinese experience and recommendations concerning detection, staging and follow-up","volume":"30","author":"Fan","year":"2020","journal-title":"Eur. Radiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.ajpath.2020.10.016","article-title":"Acute Respiratory Distress in Aged, SARS-CoV-2-Infected African Green Monkeys but Not Rhesus Macaques","volume":"191","author":"Blair","year":"2021","journal-title":"Am. J. Pathol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/s41564-020-00841-4","article-title":"Responses to acute infection with SARS-CoV-2 in the lungs of rhesus macaques, baboons and marmosets","volume":"6","author":"Singh","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1126\/science.abc6284","article-title":"DNA vaccine protection against SARS-CoV-2 in rhesus macaques","volume":"369","author":"Yu","year":"2020","journal-title":"Science"},{"key":"ref_59","first-page":"1544","article-title":"Single-shot Ad26 vaccine protects against SARS-CoV-2 in rhesus macaques","volume":"383","author":"Mercado","year":"2020","journal-title":"Nature"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"110538","DOI":"10.1016\/j.mehy.2021.110538","article-title":"Persistent SARS-2 infections contribute to long COVID-19","volume":"149","author":"Jacobs","year":"2021","journal-title":"Med. Hypotheses"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"526","DOI":"10.3389\/fmed.2020.00526","article-title":"Pulmonary and Extra-Pulmonary Clinical Manifestations of COVID-19","volume":"7","author":"Johnson","year":"2020","journal-title":"Front. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"582932","DOI":"10.3389\/fpubh.2020.582932","article-title":"Coronavirus Disease (COVID-19): Comprehensive Review of Clinical Presentation","volume":"8","author":"Mehta","year":"2020","journal-title":"Front. Public Health"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/8\/1673\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:50:08Z","timestamp":1760165408000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/13\/8\/1673"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,23]]},"references-count":62,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["v13081673"],"URL":"https:\/\/doi.org\/10.3390\/v13081673","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.11.05.369413","asserted-by":"object"}]},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,23]]}}}