{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:30:33Z","timestamp":1792027833067,"version":"4.4.0"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Special Fund for Local Science and Technology Development Guided by the Chinese\u00a0Government","award":["2018L3011"],"award-info":[{"award-number":["2018L3011"]}]},{"name":"Fujian Natural Science Foundation General Project","award":["2019J01804"],"award-info":[{"award-number":["2019J01804"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefore, this study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against FCoV using histidine-tagged recombinant spike protein. FCoV S protein (1127\u20131400 aa) was expressed and used as an antigen to establish an ELISA. Mice and rabbits immunized with the protein produced antibodies that were recognized and bound to the protein. The intra-assay coefficient of variation (CV) was 1.15\u20135.04% and the inter-assay CV was 4.28\u201315.13%, suggesting an acceptable repeatability. iELISA did not cross-react with antisera against other feline viruses. The receiver operating characteristic curve analysis revealed an 86.7% sensitivity and 93.3% specificity for iELISA. Serum samples (n = 107) were tested for anti-FCoV antibodies, and 70.09% of samples were positive for antibodies against FCoV. The iELISA developed in our study can be used to measure serum FCoV antibodies due to its acceptable repeatability, sensitivity, and specificity. Additionally, field sample analysis data demonstrated that FCoV is highly prevalent in cat populations in Fujian province, China.<\/jats:p>","DOI":"10.3390\/v13122496","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T01:22:05Z","timestamp":1639444925000},"page":"2496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus"],"prefix":"10.3390","volume":"13","author":[{"given":"Bo","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"},{"name":"Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xufei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linglin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, College of Life Science of Longyan University, Longyan 364012, China"},{"name":"Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.jfms.2008.02.006","article-title":"Pathogenesis of feline enteric coronavirus infection","volume":"10","author":"Pedersen","year":"2008","journal-title":"J. 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