{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T16:52:40Z","timestamp":1775926360314,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T00:00:00Z","timestamp":1639958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000071","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["082273"],"award-info":[{"award-number":["082273"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["079918"],"award-info":[{"award-number":["079918"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["114013"],"award-info":[{"award-number":["114013"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health","award":["U19AI128913"],"award-info":[{"award-number":["U19AI128913"]}]},{"name":"National Institute of Health","award":["P50CA107399"],"award-info":[{"award-number":["P50CA107399"]}]},{"name":"National Institute of Health","award":["75N93019C00030"],"award-info":[{"award-number":["75N93019C00030"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Maternal reinfection of immune women with novel human cytomegalovirus (HCMV) strains acquired during pregnancy can result in symptomatic congenital CMV (cCMV) infection. Novel animal model strategies are needed to explore vaccine-mediated protections against maternal reinfection. To investigate this in the guinea pig cytomegalovirus (GPCMV) model, a strictly in vivo-passaged workpool of a novel strain, the CIDMTR strain (dose, 1 \u00d7 107 pfu) was used to infect dams that had been challenged in a previous pregnancy with the 22122 strain, following either sham-immunization (vector only) or vaccination with MVA-vectored gB, gH\/gL, or pentameric complex (PC) vaccines. Maternal DNAemia cleared by day 21 in the glycoprotein-vaccinated dams, but not in the sham-immunized dams. Mean pup birth weights were 72.85 \u00b1 10.2, 80.0 \u00b1 6.9, 81.4 \u00b1 14.1, and 89.38 \u00b1 8.4 g in sham-immunized, gB, gH\/gL, and PC groups, respectively (p &lt; 0.01 for control v. PC). Pup mortality in the sham-immunized group was 6\/12 (50%), but reduced to 3\/35 (8.6%) in combined vaccine groups (p = 0.0048). Vertical CIDMTR transmission occurred in 6\/12 pups (50%) in the sham-vaccinated group, compared to 2\/34 pups (6%) in the vaccine groups (p = 0.002). We conclude that guinea pigs immunized with vectored vaccines expressing 22122 strain-specific glycoproteins are protected after a reinfection with a novel, heterologous clinical isolate (CIDMTR) in a second pregnancy.<\/jats:p>","DOI":"10.3390\/v13122551","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T04:35:57Z","timestamp":1640061357000},"page":"2551","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protection against Congenital CMV Infection Conferred by MVA-Vectored Subunit Vaccines Extends to a Second Pregnancy after Maternal Challenge with a Heterologous, Novel Strain Variant"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2210-7792","authenticated-orcid":false,"given":"Claudia","family":"Fern\u00e1ndez-Alarc\u00f3n","sequence":"first","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace","family":"Buchholz","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4476-9156","authenticated-orcid":false,"given":"Heidi","family":"Contreras","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Wussow","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don J.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA 91010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2108-9433","authenticated-orcid":false,"given":"Mark R.","family":"Schleiss","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s00430-015-0399-9","article-title":"Controversies in the natural history of congenital human cytomegalovirus infection: The paradox of infection and disease in offspring of women with immunity prior to pregnancy","volume":"204","author":"Britt","year":"2015","journal-title":"Med. 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