{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T15:05:51Z","timestamp":1772291151596,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI19\/00878"],"award-info":[{"award-number":["PI19\/00878"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 \u00b1 0.61 PFU\/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.<\/jats:p>","DOI":"10.3390\/v14010006","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T09:50:43Z","timestamp":1640080243000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4476-856X","authenticated-orcid":false,"given":"Olga","family":"Pacios","sequence":"first","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8531-6105","authenticated-orcid":false,"given":"Laura","family":"Fern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1846-4693","authenticated-orcid":false,"given":"In\u00e9s","family":"Bleriot","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4039-4142","authenticated-orcid":false,"given":"Lucia","family":"Blasco","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f3n","family":"Ambroa","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Concha","family":"Ortiz-Cartagena","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6597-6130","authenticated-orcid":false,"given":"Felipe Fern\u00e1ndez","family":"Cuenca","sequence":"additional","affiliation":[{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"},{"name":"UGC Enfermedades Infecciosas, Microbiolog\u00eda Cl\u00ednica y Medicina Preventiva, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla, 41071 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Oteo-Iglesias","sequence":"additional","affiliation":[{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"},{"name":"National Centre for Microbiology, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8672-5891","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Pascual","sequence":"additional","affiliation":[{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"},{"name":"UGC Enfermedades Infecciosas, Microbiolog\u00eda Cl\u00ednica y Medicina Preventiva, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla, 41071 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6091-4045","authenticated-orcid":false,"given":"Luis","family":"Mart\u00ednez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"},{"name":"Departamento de Microbiolog\u00eda, UGC Microbiolog\u00eda, Hospital Reina Sof\u00eda, Instituto Maim\u00f3nides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC), Universidad de C\u00f3rdoba, 14014 Cordova, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-8809","authenticated-orcid":false,"given":"Pilar","family":"Domingo-Calap","sequence":"additional","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio), Universitat de Val\u00e8ncia-CSIC, 46980 Paterna, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4501-0387","authenticated-orcid":false,"given":"Mar\u00eda","family":"Tom\u00e1s","sequence":"additional","affiliation":[{"name":"Microbiology Department-Research Institute Biomedical A Coru\u00f1a (INIBIC), Hospital A Coru\u00f1a (CHUAC), University of A Coru\u00f1a (UDC), 15006 A Coruna, Spain"},{"name":"Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), 28003 Madrid, Spain"},{"name":"Spanish Network for Research in Infectious Diseases (REIPI), 41071 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6578","DOI":"10.1073\/pnas.95.12.6578","article-title":"Prokaryotes: The unseen 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