{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:04:38Z","timestamp":1790823878152,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T00:00:00Z","timestamp":1641340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Due to their ability to trigger strong immune responses, adenoviruses (HAdVs) in general and the serotype5 (HAdV-5) in particular are amongst the most popular viral vectors in research and clinical application. However, efficient transduction using HAdV-5 is predominantly achieved in coxsackie and adenovirus receptor (CAR)-positive cells. In the present study, we used the transduction enhancer LentiBOOST\u00ae comprising the polycationic Polybrene to overcome these limitations. Using LentiBOOST\u00ae\/Polybrene, we yielded transduction rates higher than 50% in murine bone marrow-derived dendritic cells (BMDCs), while maintaining their cytokine expression profile and their capability to induce T-cell proliferation. In human dendritic cells (DCs), we increased the transduction rate from 22% in immature (i)DCs or 43% in mature (m)DCs to more than 80%, without inducing cytotoxicity. While expression of specific maturation markers was slightly upregulated using LentiBOOST\u00ae\/Polybrene on iDCs, no effect on mDC phenotype or function was observed. Moreover, we achieved efficient HAdV5 transduction also in human monocytes and were able to subsequently differentiate them into proper iDCs and functional mDCs. In summary, we introduce LentiBOOST\u00ae comprising Polybrene as a highly potent adenoviral transduction agent for new in-vitro applications in a set of different immune cells in both mice and humans.<\/jats:p>","DOI":"10.3390\/v14010092","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T23:35:09Z","timestamp":1641771309000},"page":"92","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Breaking Entry-and Species Barriers: LentiBOOST\u00ae Plus Polybrene Enhances Transduction Efficacy of Dendritic Cells and Monocytes by Adenovirus 5"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9950-4828","authenticated-orcid":false,"given":"Astrid","family":"Strack","sequence":"first","affiliation":[{"name":"Department of Immune Modulation, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Deinzer","sequence":"additional","affiliation":[{"name":"Department of Immune Modulation, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"},{"name":"Institute of Clinical Microbiology, Immunology and Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Wasserturmstra\u00dfe 3\/5, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Thirion","sequence":"additional","affiliation":[{"name":"SIRION Biotech GmbH, Am Klopferspitz 19, 82152 Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Schr\u00f6del","sequence":"additional","affiliation":[{"name":"SIRION Biotech GmbH, Am Klopferspitz 19, 82152 Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3478-0741","authenticated-orcid":false,"given":"Jan","family":"D\u00f6rrie","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Sauerer","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Steinkasserer","sequence":"additional","affiliation":[{"name":"Department of Immune Modulation, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka","family":"Knippertz","sequence":"additional","affiliation":[{"name":"Department of Immune Modulation, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander Universit\u00e4t Erlangen-N\u00fcrnberg, Hartmannstr. 14, 91052 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.gendis.2017.04.001","article-title":"Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine","volume":"4","author":"Lee","year":"2017","journal-title":"Genes Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Crenshaw, B.J., Jones, L.B., Bell, C.R., Kumar, S., and Matthews, Q.L. 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