{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:41:01Z","timestamp":1775911261773,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Human infections caused by the H5 highly pathogenic avian influenza virus (HPAIV) sporadically threaten public health. The susceptibility of HPAIVs to baloxavir acid (BXA), a new class of inhibitors for the influenza virus cap-dependent endonuclease, has been confirmed in vitro, but it has not yet been fully characterized. Here, the efficacy of BXA against HPAIVs, including recent H5N8 variants, was assessed in vitro. The antiviral efficacy of baloxavir marboxil (BXM) in H5N1 virus-infected mice was also investigated. BXA exhibited similar in vitro activities against H5N1, H5N6, and H5N8 variants tested in comparison with seasonal and other zoonotic strains. Compared with oseltamivir phosphate (OSP), BXM monotherapy in mice infected with the H5N1 HPAIV clinical isolate, the A\/Hong Kong\/483\/1997 strain, also caused a significant reduction in viral titers in the lungs, brains, and kidneys, thereby preventing acute lung inflammation and reducing mortality. Furthermore, compared with BXM or OSP monotherapy, combination treatments with BXM and OSP using a 48-h delayed treatment model showed a more potent effect on viral replication in the organs, accompanied by improved survival. In conclusion, BXM has a potent antiviral efficacy against H5 HPAIV infections.<\/jats:p>","DOI":"10.3390\/v14010111","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T23:35:09Z","timestamp":1641771309000},"page":"111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Characterization of the In Vitro and In Vivo Efficacy of Baloxavir Marboxil against H5 Highly Pathogenic Avian Influenza Virus Infection"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4694-2146","authenticated-orcid":false,"given":"Keiichi","family":"Taniguchi","sequence":"first","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"},{"name":"Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Ando","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinsuke","family":"Toba","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"},{"name":"International Institute for Zoonosis Control, Hokkaido University, Hokkaido 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5735-0843","authenticated-orcid":false,"given":"Haruaki","family":"Nobori","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8847-0071","authenticated-orcid":false,"given":"Takao","family":"Sanaki","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Noshi","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kawai","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryu","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"},{"name":"International Institute for Zoonosis Control, Hokkaido University, Hokkaido 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2549-0945","authenticated-orcid":false,"given":"Takao","family":"Shishido","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Naito","sequence":"additional","affiliation":[{"name":"Shionogi & Co., Ltd., Osaka 561-0825, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keita","family":"Matsuno","sequence":"additional","affiliation":[{"name":"International Institute for Zoonosis Control, Hokkaido University, Hokkaido 001-0020, Japan"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Hokkaido 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Okamatsu","sequence":"additional","affiliation":[{"name":"Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7021-1688","authenticated-orcid":false,"given":"Yoshihiro","family":"Sakoda","sequence":"additional","affiliation":[{"name":"Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Hokkaido 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kida","sequence":"additional","affiliation":[{"name":"International Institute for Zoonosis Control, Hokkaido University, Hokkaido 001-0020, Japan"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Hokkaido 001-0020, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2021, August 14). 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