{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:29:29Z","timestamp":1787614169489,"version":"build-2736575974"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T00:00:00Z","timestamp":1644883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Budget","award":["121051900147-5"],"award-info":[{"award-number":["121051900147-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>It has been established that blood vessels are a target for influenza virus; however, the mechanism by which virus affects the cardiovascular system remains unknown. The aim of the study is the identification of histological changes and changes in the functional activity of the pulmonary and mesenteric blood vessels of Wistar rats. Wistar rats were intranasally infected with the influenza A(H1N1)pdm09 virus. At 24 and 96 h post infection (hpi), histopathological changes were observed in lung tissues with the absence of histological changes in mesenteric tissues. The functional activity of pulmonary and mesenteric arteries was determined using wire myography. In pulmonary arteries, there was a tendency towards an increase in integral response to the vasodilator and a decrease in the integral response to the vasoconstrictor at 24 hpi (compared with control). At 96 hpi, a tendency towards a decrease in the integral response to the vasoconstrictor persisted, while the response to acetylcholine was slightly increased. The functional activity of the mesenteric blood vessels was inverted: a significant decrease in the integral response to the vasodilator and an increase in the response to the vasoconstrictor at 24 hpi were observed; at 96 hpi, the integral response to the vasoconstrictor persisted, while the response to the vasodilator remained significantly reduced. Obtained data indicate the development of endothelial dysfunction in non-lethal and clinically non-severe experimental influenza virus infection.<\/jats:p>","DOI":"10.3390\/v14020396","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T22:44:57Z","timestamp":1644965097000},"page":"396","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Influenza A Virus Causes Histopathological Changes and Impairment in Functional Activity of Blood Vessels in Different Vascular Beds"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-3157","authenticated-orcid":false,"given":"Vladimir","family":"Marchenko","sequence":"first","affiliation":[{"name":"Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1971-3444","authenticated-orcid":false,"given":"Irina","family":"Zelinskaya","sequence":"additional","affiliation":[{"name":"Almazov National Medical Research Centre, Russian Ministry of Health, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1629-7868","authenticated-orcid":false,"given":"Yana","family":"Toropova","sequence":"additional","affiliation":[{"name":"Almazov National Medical Research Centre, Russian Ministry of Health, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Shmakova","sequence":"additional","affiliation":[{"name":"Almazov National Medical Research Centre, Russian Ministry of Health, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0365-3301","authenticated-orcid":false,"given":"Ekaterina","family":"Podyacheva","sequence":"additional","affiliation":[{"name":"Almazov National Medical Research Centre, Russian Ministry of Health, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3643-7354","authenticated-orcid":false,"given":"Dmitry","family":"Lioznov","sequence":"additional","affiliation":[{"name":"Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina N.","family":"Zhilinskaya","sequence":"additional","affiliation":[{"name":"Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/irv.12241","article-title":"Neurological events related to influenza A (H1N1) pdm09","volume":"8","author":"Ugalde","year":"2014","journal-title":"Influenza Other Respir. Viruses"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.amjmed.2007.10.040","article-title":"Complications of Viral Influenza","volume":"121","author":"Rothberg","year":"2008","journal-title":"Am. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1056\/NEJMoa1702090","article-title":"Acute Myocardial Infarction after Laboratory-Confirmed Influenza Infection","volume":"378","author":"Kwong","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_4","first-page":"25","article-title":"Modern viewpoints on cardiovascular disturbances in influenza","volume":"11","author":"Lyon","year":"1952","journal-title":"Acta Med. Orient."},{"key":"ref_5","first-page":"15","article-title":"Some aspects of the study on circulatory disorders in influenza","volume":"17","author":"Sergeev","year":"1962","journal-title":"Vestn. Akad. Meditsinskikh Nauk. SSSR"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1146\/annurev.pathmechdis.3.121806.154316","article-title":"The pathology of influenza virus infections","volume":"3","author":"Taubenberger","year":"2008","journal-title":"Annu. Rev. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jcv.2019.05.003","article-title":"Excess mortality is associated with influenza A (H1N1) in patients with severe acute respiratory illness","volume":"116","author":"Lobo","year":"2019","journal-title":"J. Clin. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s13730-014-0147-9","article-title":"A case of acute kidney injury and disseminated intravascular coagulation associated with influenza B viral infection","volume":"4","author":"Fukunaga","year":"2014","journal-title":"CEN Case Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00467-003-1143-z","article-title":"Renal involvement in children with influenza A virus infection","volume":"18","author":"Watanabe","year":"2003","journal-title":"Pediatr. Nephrol."},{"key":"ref_10","first-page":"75","article-title":"Hypertoxic flu","volume":"2","author":"Sulima","year":"2010","journal-title":"J. Infectol."},{"key":"ref_11","first-page":"93","article-title":"Rare symptoms (diarrhea, meningism, hemor rhinic syndrome) in patients with different influenza virus subtypes","volume":"71","author":"Esaulenko","year":"2013","journal-title":"Vestnik NovSU."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1001\/jama.2020.21869","article-title":"High-risk Groups for Influenza Complications","volume":"324","author":"Uyeki","year":"2020","journal-title":"JAMA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e2531","DOI":"10.7717\/peerj.2531","article-title":"Did the 1918 influenza cause the twentieth century cardiovascular mortality epidemic in the United States?","volume":"4","author":"Tate","year":"2016","journal-title":"PeerJ"},{"key":"ref_14","first-page":"14","article-title":"Spanish flu and early 20th-century expansion of a coronary heart disease-prone subpopulation","volume":"31","author":"Azambuja","year":"2004","journal-title":"Tex. Heart Inst. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2147\/JRLCR.S140624","article-title":"Sialic acid receptors: Focus on their role in influenza infection","volume":"10","year":"2018","journal-title":"J. Recept. Ligand Channel Res."},{"key":"ref_16","first-page":"20","article-title":"Influenza virus reproduction in the endothelial cells of human blood vessels","volume":"57","author":"Zhilinskaya","year":"2012","journal-title":"Probl. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3389\/fmicb.2014.00653","article-title":"Influenza virus and endothelial cells: A species specific relationship","volume":"5","author":"Short","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"537","DOI":"10.4161\/viru.25779","article-title":"Endothelial activation and dysfunction in the pathogenesis of influenza A virus infection","volume":"4","author":"Armstrong","year":"2013","journal-title":"Virulence"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.36233\/0507-4088-48","article-title":"Modulation of endothelial factors activity in human endothelial cells in influenza A(H1N1)pdm09 virus infection","volume":"66","author":"Marchenko","year":"2021","journal-title":"Probl. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-70753-0","article-title":"Modelling the linkage between influenza infection and cardiovascular events via thrombosis","volume":"10","author":"McCarthy","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"159","DOI":"10.36233\/0507-4088-2020-65-3-159-166","article-title":"Modeling influenza virus infection in mature Wistar rats","volume":"65","author":"Marchenko","year":"2020","journal-title":"Probl. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"86","DOI":"10.24884\/1682-6655-2019-18-1-86-95","article-title":"Model of chronic thromboembolic pulmonary hypertension in rats, caused by repeated intravenous administration of biodegradable microspheres from sodium alginate","volume":"18","author":"Karpov","year":"2019","journal-title":"Reg. Blood Circ. Microcirc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1007\/s00210-020-01873-7","article-title":"Comparative efficacy of empagliflozin and drugs of baseline therapy in post-infarct heart failure in normoglycemic rats","volume":"393","author":"Krasnova","year":"2020","journal-title":"Naunyn-Schmiedebergs Arch. Exp. Pathol. Pharmakol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"71","DOI":"10.15829\/1560-4071-2018-3-71-75","article-title":"Functional state of myocardium after application of a non-cellular allogenic material for stimulation of regeneration capacity in experimental infarction","volume":"3","author":"Afanasiev","year":"2018","journal-title":"Russ. J. Cardiol."},{"key":"ref_25","first-page":"21","article-title":"Pathogenic aspects of influenza during the epidemics caused by H1N1v virus in 2009\u20132010","volume":"73","author":"Tsinzerling","year":"2011","journal-title":"Arkhiv Patologii."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_27","first-page":"293","article-title":"Kinetic profile of influenza virus infection in three rat strains","volume":"53","author":"Mary","year":"2003","journal-title":"Comp. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1177\/030098589603300407","article-title":"Histopathological Changes in the Upper Respiratory Tract of F344 Rats Following Infection with a Rat-adapted Influenza Virus","volume":"33","author":"Ibanes","year":"1996","journal-title":"Vet. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/872370","article-title":"Mechanisms of Action and Efficacy of Statins against Influenza","volume":"2014","author":"Mehrbod","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kwong, J.C., Li, P., and Redelmeier, D.A. (2009). Influenza morbidity and mortality in elderly patients receiving statins: A cohort study. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0008087"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1017\/S0950268810000105","article-title":"An assessment of the effect of statin use on the incidence of acute respiratory infections in England during winters 1998\u20131999 to 2005\u20132006","volume":"138","author":"Fleming","year":"2010","journal-title":"Epidemiol. 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