{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:18Z","timestamp":1792790178895,"version":"4.5.0"},"reference-count":19,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["NCT02970227"],"award-info":[{"award-number":["NCT02970227"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020407","name":"ERA-CVD","doi-asserted-by":"publisher","award":["JTC2016-40-158"],"award-info":[{"award-number":["JTC2016-40-158"]}],"id":[{"id":"10.13039\/501100020407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ProFIT grant of the Investitionsbank Berlin\/cofounded of EFRE","award":["10169096, 10169098, 10169028"],"award-info":[{"award-number":["10169096, 10169098, 10169028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Human parvovirus B19 (B19V) is the predominant virus currently detected in endomyocardial biopsies (EMBs). Recent findings indicate that, specifically, transcriptionally active B19V with detectable viral RNA is of prognostic relevance in inflammatory viral cardiomyopathy. We aimed to evaluate B19V replicative status (viral RNA) and beneficial effects in a sub-collective of the prospective randomized placebo-controlled phase II multi-center BICC-Trial (Betaferon In Chronic Viral Cardiomyopathy) after interferon beta-1b (IFN-\u03b2) treatment. EMBs of n = 64 patients with B19V mono-infected tissue were retrospectively analyzed. Viral RNA could be detected in n = 18\/64 (28.1%) of B19V DNA positive samples (mean age 51.7 years, 12 male), of whom n = 13 had been treated with IFN-\u00df. Five patients had received placebo. PCR analysis confirmed in follow-up that EMBs significantly reduced viral RNA loads in n = 11\/13 (84.6%) of IFN-\u00df treated patients (p = 0.001), independently from the IFN-\u00df dose, in contrast to the placebo group, where viral RNA load was not affected or even increased. Consequently, a significant improvement of left ventricular ejection fraction (LVEF) after treatment with IFN-\u00df was observed (LVEF mean baseline 51.6 \u00b1 14.1% vs. follow-up 61.0 \u00b1 17.5%, p = 0.03). In contrast, in the placebo group, worsening of LVEF was evaluated in n = 4\/5 (80.0%) of patients. We could show for the first-time the beneficial effects from treatment with IFN-\u00df, suppressing B19V viral RNA and improving the hemodynamic course. Our results need further verification in a larger prospective randomized controlled trial.<\/jats:p>","DOI":"10.3390\/v14020444","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:48:41Z","timestamp":1645476521000},"page":"444","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Interferon-\u03b2 Suppresses Transcriptionally Active Parvovirus B19 Infection in Viral Cardiomyopathy: A Subgroup Analysis of the BICC-Trial"],"prefix":"10.3390","volume":"14","author":[{"given":"Heinz-Peter","family":"Schultheiss","sequence":"first","affiliation":[{"name":"Institute of Cardiac Diagnostics and Therapy, IKDT GmbH, 12203 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2773-486X","authenticated-orcid":false,"given":"Claus-Thomas","family":"Bock","sequence":"additional","affiliation":[{"name":"Division of Viral Gastroenteritis and Hepatitis Pathogens and Enteroviruses, Department of Infectious Diseases, Robert Koch Institute, 13353 Berlin, Germany"},{"name":"Institute of Tropical Medicine, University of Tuebingen, 72074 Tuebingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganna","family":"Aleshcheva","sequence":"additional","affiliation":[{"name":"Institute of Cardiac Diagnostics and Therapy, IKDT GmbH, 12203 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baumeier","sequence":"additional","affiliation":[{"name":"Institute of Cardiac Diagnostics and Therapy, IKDT GmbH, 12203 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Poller","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Campus Benjamin Franklin, Charit\u00e9, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Universitaetsmedizin Berlin, Corporate Member of Freie Universitaet Berlin and Humboldt-Universitaet zu Berlin, 13353 Berlin, Germany"},{"name":"DZHK (German Centre for Cardiovascular Research), Partner Site, 10785 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0678-5681","authenticated-orcid":false,"given":"Felicitas","family":"Escher","sequence":"additional","affiliation":[{"name":"Institute of Cardiac Diagnostics and Therapy, IKDT GmbH, 12203 Berlin, Germany"},{"name":"DZHK (German Centre for Cardiovascular Research), Partner Site, 10785 Berlin, Germany"},{"name":"Department of Internal Medicine and Cardiology, Campus Virchow-Klinikum, Charit\u00e9, Universitaetsmedizin Berlin, Corporate Member of Freie Universitaet Berlin and Humboldt-Universitaet zu Berlin, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1056\/NEJMc0911362","article-title":"Human Parvovirus B19\u2013Associated Myocarditis","volume":"362","author":"Bock","year":"2010","journal-title":"N. 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Heart J."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/2\/444\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:24:21Z","timestamp":1760135061000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/2\/444"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,21]]},"references-count":19,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["v14020444"],"URL":"https:\/\/doi.org\/10.3390\/v14020444","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,21]]}}}