{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:54:37Z","timestamp":1760151277586,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072858"],"award-info":[{"award-number":["32072858"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Ten Thousand Talents Program for Young Talents","award":["W03070106"],"award-info":[{"award-number":["W03070106"]}]},{"name":"Outstanding Talents of Henan Agricultural University","award":["30600773"],"award-info":[{"award-number":["30600773"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Pseudorabies virus (PRV) is a contagious herpesvirus that causes Aujeszky\u2019s disease and economic losses worldwide. Liver X receptors (LXRs) belong to the nuclear receptor superfamily and are critical for the control of lipid homeostasis. However, the role of LXR in PRV infection has not been fully established. In this study, we found that PRV infection downregulated the mRNA and protein levels of LXR\u03b1 and LXR\u03b2 in vitro and in vivo. Furthermore, we discovered that LXR activation suppressed PRV proliferation, while LXR inhibition promoted PRV proliferation. We demonstrated that LXR activation-mediated reduction of cellular cholesterol was critical for the dynamics of PRV entry-dependent clathrin-coated pits. Replenishment of cholesterol restored the dynamics of clathrin-coated pits and PRV entry under LXR activation conditions. Interestingly, T0901317, an LXR agonist, prevented PRV infection in mice. Our results support a model that PRV modulates LXR-regulated cholesterol metabolism to facilitate viral proliferation.<\/jats:p>","DOI":"10.3390\/v14030514","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T20:36:30Z","timestamp":1646339790000},"page":"514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection"],"prefix":"10.3390","volume":"14","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Li","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Li","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao-Xue","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Growth and Development, Zhengzhou 450046, China"},{"name":"International Joint Research Center of National Animal Immunology, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"College of Animal Science & Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450047, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic 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