{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:59:54Z","timestamp":1790837994066,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Although the respiratory tract is the main target of SARS-CoV-2, other tissues and organs are permissive to the infection. In this report, we investigated this wide-spectrum tropism by studying the SARS-CoV-2 genetic intra-host variability in multiple tissues. The virological and histological investigation of multiple specimens from a post-mortem COVID-19 patient was performed. SARS-CoV-2 genome was detected in several tissues, including the lower respiratory system, cardio-vascular biopsies, stomach, pancreas, adrenal gland, mediastinal ganglion and testicles. Subgenomic RNA transcripts were also detected, in favor of an active viral replication, especially in testicles. Ultra-deep sequencing allowed us to highlight several SARS-CoV-2 mutations according to tissue distribution. More specifically, mutations of the spike protein, i.e., V341A (18.3%), E654 (44%) and H655R (30.8%), were detected in the inferior vena cava. SARS-CoV-2 variability can contribute to heterogeneous distributions of viral quasispecies, which may affect the COVID-19 pathogeny.<\/jats:p>","DOI":"10.3390\/v14030515","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T20:36:30Z","timestamp":1646339790000},"page":"515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multiorgan and Vascular Tropism of SARS-CoV-2"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5942-2345","authenticated-orcid":false,"given":"C\u00e9dric","family":"Hartard","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Department of Microbiology, CHRU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"},{"name":"CNRS, LCPME, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahlam","family":"Chaqroun","sequence":"additional","affiliation":[{"name":"CNRS, LCPME, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3107-2771","authenticated-orcid":false,"given":"Nicla","family":"Settembre","sequence":"additional","affiliation":[{"name":"Department of Vascular & Endovascular Surgery, CHRU Nancy, Universit\u00e9 de Lorraine, INSERM 1116, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5585-9195","authenticated-orcid":false,"given":"Guillaume","family":"Gauchotte","sequence":"additional","affiliation":[{"name":"Department of Biopathology, Centre de Ressources Biologiques, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lefevre","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, CHRU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"},{"name":"APEMAC, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Marchand","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, CHU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-0986","authenticated-orcid":false,"given":"Charles","family":"Mazeaud","sequence":"additional","affiliation":[{"name":"Department of Urology, CHRU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc Trung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Service ORL, CHRU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7390-8947","authenticated-orcid":false,"given":"Laurent","family":"Martrille","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, CHU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0436-486X","authenticated-orcid":false,"given":"Isabelle","family":"Koscinski","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie de la Reproduction\/CECOS Lorraine, CHRU Nancy, INSERM U1256 NGERE, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergue\u00ef","family":"Malikov","sequence":"additional","affiliation":[{"name":"Department of Vascular & Endovascular Surgery, CHRU Nancy, Universit\u00e9 de Lorraine, INSERM 1116, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Schvoerer","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Department of Microbiology, CHRU Nancy, Universit\u00e9 de Lorraine, F-54000 Nancy, France"},{"name":"CNRS, LCPME, Universit\u00e9 de Lorraine, F-54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1056\/NEJMoa2015432","article-title":"Pulmonary Vascular Endothelialitis, Thrombosis, and Angiogenesis in Covid-19","volume":"383","author":"Ackermann","year":"2020","journal-title":"N. 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