{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:45:06Z","timestamp":1790768706227,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T00:00:00Z","timestamp":1648339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["304630\/2013-6"],"award-info":[{"award-number":["304630\/2013-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2015\/13818-7"],"award-info":[{"award-number":["2015\/13818-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Pathogenicity and pathology of rabies virus (RABV) varies according to the variant, but the mechanisms are not completely known. In this study, gene expression profile in brains of mice experimentally infected with RABV isolated from a human case of dog rabies (V2) or vampire bat-acquired rabies (V3) were analyzed. In total, 138 array probes associated with 120 genes were expressed differentially between mice inoculated with V2 and sham-inoculated control mice at day 10 post-inoculation. A single probe corresponding to an unannotated gene was identified in V3 versus control mice. Gene ontology (GO) analysis revealed that all of the genes upregulated in mice inoculated with V2 RABV were involved in the biological process of immune defense against pathogens. Although both variants are considered pathogenic, inoculation by the same conditions generated different gene expression results, which is likely due to differences in pathogenesis between the dog and bat RABV variants. This study demonstrated the global gene expression in experimental infection due to V3 wild-type RABV, from the vampire bat Desmodus rotundus, an important source of infection for humans, domestic animals and wildlife in Latin America.<\/jats:p>","DOI":"10.3390\/v14040692","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:31:25Z","timestamp":1648416685000},"page":"692","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Camila M.","family":"Appolin\u00e1rio","sequence":"first","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Estadual Paulista, Julio de Mesquita Filho, Distrito de Rubi\u00e3o J\u00fanior, s\/n, CEP, Botucatu 18618-970, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1912-4500","authenticated-orcid":false,"given":"Janet M.","family":"Daly","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6855-5481","authenticated-orcid":false,"given":"Richard D.","family":"Emes","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio Albuquerque","family":"Marchi","sequence":"additional","affiliation":[{"name":"International Research Center, A.C Camargo Cancer Center, S\u00e3o Paulo 01509-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Leticia Devid\u00e9","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Estadual Paulista, Julio de Mesquita Filho, Distrito de Rubi\u00e3o J\u00fanior, s\/n, CEP, Botucatu 18618-970, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Megid","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Estadual Paulista, Julio de Mesquita Filho, Distrito de Rubi\u00e3o J\u00fanior, s\/n, CEP, Botucatu 18618-970, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hampson, K., Coudeville, L., Lembo, T., Sambo, M., Kieffer, A., Attlan, M., Barrat, J., Blanton, J.D., Briggs, D.J., and Cleaveland, S. (2015). Estimating the global burden of endemic canine rabies. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003786"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"524","DOI":"10.15585\/mmwr.mm6823e1","article-title":"Vital Signs: Trends in Human Rabies Deaths and Exposures\u2014United States, 1938\u20132018","volume":"68","author":"Pieracci","year":"2019","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"678","DOI":"10.3201\/eid1904.121482","article-title":"Rabies update for Latin America and the Caribbean","volume":"19","author":"Vigilato","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"12554","DOI":"10.1128\/JVI.79.19.12554-12565.2005","article-title":"Attenuated rabies virus activates, while pathogenic rabies virus evades, the host innate immune responses in the central nervous system","volume":"79","author":"Wang","year":"2005","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/S1474-4422(13)70038-3","article-title":"Human rabies: Neuropathogenesis, diagnosis, and management","volume":"12","author":"Hemachudha","year":"2013","journal-title":"Lancet Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1080\/13550280802216502","article-title":"Neuronal apoptosis does not play an important role in human rabies encephalitis","volume":"14","author":"Jackson","year":"2008","journal-title":"J. Neurovirol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1128\/JVI.73.1.510-518.1999","article-title":"Pathogenicity of different rabies virus variants inversely correlates with apoptosis and rabies virus glycoprotein expression in infected primary neuron cultures","volume":"73","author":"Morimoto","year":"1999","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1093\/cid\/cit372","article-title":"Clinical features of dog- and bat-acquired rabies in humans","volume":"57","author":"Udow","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"378","DOI":"10.4269\/ajtmh.15-0361","article-title":"Profile of Cytokines and Chemokines Triggered by Wild-Type Strains of Rabies Virus in Mice","volume":"94","author":"Appolinario","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"618","DOI":"10.2144\/04364ST02","article-title":"Comparison of mRNA gene expression by RT-PCR and DNA microarray","volume":"36","author":"Etienne","year":"2004","journal-title":"Biotechniques"},{"key":"ref_11","first-page":"479","article-title":"Fluorescent antibody test, quantitative polymerase chain reaction pattern and clinical aspects of rabies virus strains isolated from main reservoirs in Brazil","volume":"19","author":"Appolinario","year":"2015","journal-title":"Braz. J. Infect. Dis. Off. Publ. Braz. Soc. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.bjid.2015.05.008","article-title":"Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus","volume":"19","author":"Appolinario","year":"2015","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1007\/s00705-019-04335-5","article-title":"Nyctinomops laticaudatus bat-associated Rabies virus causes disease with a shorter clinical period and has lower pathogenic potential than strains isolated from wild canids","volume":"164","author":"Fuoco","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1348-0421.2006.tb03871.x","article-title":"Screening of upregulated genes in suckling mouse central nervous system during the disease stage of rabies virus infection","volume":"50","author":"Ubol","year":"2006","journal-title":"Microbiol. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7181","DOI":"10.1128\/JVI.72.9.7181-7190.1998","article-title":"The neural cell adhesion molecule is a receptor for rabies virus","volume":"72","author":"Thoulouze","year":"1998","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s13365-011-0041-6","article-title":"The type I interferon response bridles rabies virus infection and reduces pathogenicity","volume":"17","author":"Chopy","year":"2011","journal-title":"J. Neurovirol."},{"key":"ref_17","first-page":"751","article-title":"Genome-Wide Transcriptional Profiling Reveals Two Distinct Outcomes in Central Nervous System Infections of Rabies Virus","volume":"7","author":"Zhang","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7506","DOI":"10.4049\/jimmunol.180.11.7506","article-title":"Detrimental contribution of the immuno-inhibitor B7-H1 to Rabies Virus encephalitis","volume":"180","author":"Lafon","year":"2008","journal-title":"J. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"463","DOI":"10.7883\/yoken.64.463","article-title":"Gene expression analysis of host immune response in the central nervous system following lethal CVS-11 infection in mice","volume":"64","author":"Sugiura","year":"2011","journal-title":"Jpn J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10063","DOI":"10.1128\/JVI.79.15.10063-10068.2005","article-title":"Degeneration of neuronal processes after infection with pathogenic, but not attenuated, rabies viruses","volume":"79","author":"Li","year":"2005","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Feige, L., Zaeck, L.M., Sehl-Ewert, J., Finke, S., and Bourhy, H. (2021). Innate Immune Signaling and Role of Glial Cells in Herpes Simplex Virus- and Rabies Virus-Induced Encephalitis. Viruses, 13.","DOI":"10.3390\/v13122364"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0163-4453(88)91767-7","article-title":"Rabies presenting with myocarditis and encephalitis","volume":"17","author":"Raman","year":"1988","journal-title":"J. Infect."},{"key":"ref_23","first-page":"339","article-title":"Rabies encephalomyelitis with myocarditis and pancreatitis. Report on a case recently imported into France","volume":"12","author":"Hofman","year":"1992","journal-title":"Ann. Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1146\/annurev-animal-022516-022801","article-title":"Vomeronasal Receptors in Vertebrates and the Evolution of Pheromone Detection","volume":"5","author":"Silva","year":"2017","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_25","first-page":"677","article-title":"Imaging Findings in Rabies Encephalitis","volume":"22","author":"Awasthi","year":"2001","journal-title":"Am. J. Neuroradiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.virol.2005.01.023","article-title":"The vomeronasal chemosensory system as a route of neuroinvasion by herpes simplex virus","volume":"334","author":"Mori","year":"2005","journal-title":"Virology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/0042-6822(91)90145-2","article-title":"Spread of the CVS strain of rabies virus and of the avirulent mutant AvO1 along the olfactory pathways of the mouse after intranasal inoculation","volume":"183","author":"Lafay","year":"1991","journal-title":"Virology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6657","DOI":"10.1128\/JVI.00302-11","article-title":"Ambivalent role of innate immune response in rabies virus pathogenesis","volume":"85","author":"Chopy","year":"2011","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1099\/vir.0.18826-0","article-title":"Common host genes are activated in mouse brain by Japanese encephalitis and rabies virus","volume":"84","author":"Saha","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7666","DOI":"10.4049\/jimmunol.176.12.7666","article-title":"Regional differenced in blood-brain barrier permeability changes and inflammation in the apathogenic clearance of virus from the central nervous system","volume":"176","author":"Phares","year":"2006","journal-title":"J. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jneuroim.2009.09.019","article-title":"Correlation of cytokine expression with rabies virus distribution in rabies encephalitis","volume":"217","author":"Solanki","year":"2009","journal-title":"J. Neuroimmunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"11808","DOI":"10.1128\/JVI.01346-09","article-title":"The roles of chemokines in rabies virus infection: Overexpression may not always be beneficial","volume":"83","author":"Zhao","year":"2009","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"12893","DOI":"10.1128\/JVI.79.20.12893-12904.2005","article-title":"Virus infection switches TLR-3 positive human neurons into high producers of interferon-beta","volume":"79","author":"Phehaud","year":"2005","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.virusres.2009.03.014","article-title":"Role of chemokines in the enhancement of BBB permeability and inflammatory infiltration after rabies virus infection","volume":"144","author":"Kuang","year":"2009","journal-title":"Virus Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4002","DOI":"10.1128\/JVI.02220-09","article-title":"Rabies virus nucleoprotein functions to evade activation of the RIG-I-mediated antiviral response","volume":"84","author":"Matasani","year":"2010","journal-title":"J. Virol."},{"key":"ref_36","first-page":"56","article-title":"Rabies virus clearance from the central nervous system","volume":"79","author":"Hooper","year":"2011","journal-title":"Adv. Virus Res."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/4\/692\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:44:31Z","timestamp":1760136271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/4\/692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,27]]},"references-count":36,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["v14040692"],"URL":"https:\/\/doi.org\/10.3390\/v14040692","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,27]]}}}