{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:34Z","timestamp":1792790194813,"version":"4.5.0"},"reference-count":19,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shibiao Yang","award":["2017YFD0501800"],"award-info":[{"award-number":["2017YFD0501800"]}]},{"name":"Xianghua Shu","award":["202102AE090007"],"award-info":[{"award-number":["202102AE090007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiological investigations of PRV in pig populations from Yunnan Province during 2017\u20132021 were performed. The results showed that 31.37% (6324\/20,158, 95% CI 30.73\u201332.01) of serum samples were positive for PRV glycoprotein E (gE)-specific antibodies via enzyme-linked immunosorbent assay (ELISA). The risk factors, including the breeding scale and development stage, were significantly associated with PRV seroprevalence among pigs in Yunnan Province. Of the 416 tissue samples collected from PRV-suspected pigs in Yunnan Province, 43 (10.33%, 95% CI 7.41\u201313.26) samples were positive for PRV-gE nucleic acid in which 15 novel PRV strains from these PRV-positive samples were isolated, whose gC and gE sequences were analyzed. Phylogenetic analysis showed that all 15 isolates obtained in this study belonged to the genotype II. Additionally, the gC gene of one isolate (YuN-YL-2017) was genetically closer to variant PRV strains compared with others, while the gE gene was in the same clade with other classical PRV strains, indicating that this isolate might be a recombinant strain generated from the classical and variant strains. The results revealed the severe PRV epidemic in Yunnan Province and indicated that PRV variants are the major genotypes threatening the pig industry development.<\/jats:p>","DOI":"10.3390\/v14050895","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T02:14:39Z","timestamp":1650939279000},"page":"895","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021"],"prefix":"10.3390","volume":"14","author":[{"given":"Jun","family":"Yao","sequence":"first","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Yunnan Sino-Science Gene Technology Co., Ltd., Kunming 650501, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Gao","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"He","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarui","family":"Xie","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Sino-Science Gene Technology Co., Ltd., Kunming 650501, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luoyan","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlian","family":"Song","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghua","family":"Shu","sequence":"additional","affiliation":[{"name":"Yunnan Sino-Science Gene Technology Co., Ltd., Kunming 650501, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2015.12.008","article-title":"Control of swine pseudorabies in China: Opportunities and limitations","volume":"183","author":"Sun","year":"2016","journal-title":"Vet. 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