{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:27:38Z","timestamp":1789759658737,"version":"4.0.1"},"reference-count":18,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>African swine fever (ASF) is the cause of a recent pandemic that is posing a threat to much of the world swine production. The etiological agent, ASF virus (ASFV), infects domestic and wild swine, producing a variety of clinical presentations depending on the virus strain and the genetic background of the pigs infected. No commercial vaccine is currently available, although recombinant live attenuated vaccine candidates have been shown to be efficacious. In addition to determining efficacy, it is paramount to evaluate the safety profile of a live attenuated vaccine. The presence of residual virulence and the possibility of reversion to virulence are two of the concerns that must be evaluated in the development of live attenuated vaccines. Here we evaluate the safety profile of an efficacious live attenuated vaccine candidate, ASFV-G-\u0394I177L. Results from safety studies showed that ASFV-G-\u0394I177L remains genetically stable and phenotypically attenuated during a five-passage reversion to virulence study in domestic swine. In addition, large-scale experiments to detect virus shedding and transmission confirmed that even under varying conditions, ASFV-G-\u0394I177L is a safe live attenuated vaccine.<\/jats:p>","DOI":"10.3390\/v14050896","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T02:14:39Z","timestamp":1650939279000},"page":"896","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":94,"title":["Evaluation of the Safety Profile of the ASFV Vaccine Candidate ASFV-G-\u0394I177L"],"prefix":"10.3390","volume":"14","author":[{"given":"Xuan Hanh","family":"Tran","sequence":"first","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Thi Thu","family":"Phuong","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Quang","family":"Huy","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Thanh","family":"Thuy","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Dung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pham H\u00e0o","family":"Quang","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qu\u00e1ch V\u00f5","family":"Ng\u00f4n","sequence":"additional","affiliation":[{"name":"National Veterinary Joint Stock Company (NAVETCO), Ho Chi Minh City 70000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayushi","family":"Rai","sequence":"additional","affiliation":[{"name":"Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, NY 11944, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril G.","family":"Gay","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7894-0233","authenticated-orcid":false,"given":"Douglas Paul","family":"Gladue","sequence":"additional","affiliation":[{"name":"Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, NY 11944, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel Victor","family":"Borca","sequence":"additional","affiliation":[{"name":"Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, NY 11944, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1111\/tbed.14341","article-title":"African swine fever in the Dominican Republic","volume":"68","author":"Gonzales","year":"2021","journal-title":"Transbound Emerg. 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