{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:01:14Z","timestamp":1790611274552,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T00:00:00Z","timestamp":1651276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2021B0202040002"],"award-info":[{"award-number":["2021B0202040002"]}]},{"name":"Key-Area Research and Development Program of Guangdong Province","award":["NT2021008"],"award-info":[{"award-number":["NT2021008"]}]},{"name":"Key-Area Research and Development Program of Guangdong Province","award":["2021KJ150"],"award-info":[{"award-number":["2021KJ150"]}]},{"name":"Laboratory of Lingnan Modern Agriculture Project","award":["2021B0202040002"],"award-info":[{"award-number":["2021B0202040002"]}]},{"name":"Laboratory of Lingnan Modern Agriculture Project","award":["NT2021008"],"award-info":[{"award-number":["NT2021008"]}]},{"name":"Laboratory of Lingnan Modern Agriculture Project","award":["2021KJ150"],"award-info":[{"award-number":["2021KJ150"]}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["2021B0202040002"],"award-info":[{"award-number":["2021B0202040002"]}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["NT2021008"],"award-info":[{"award-number":["NT2021008"]}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["2021KJ150"],"award-info":[{"award-number":["2021KJ150"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Largemouth bass virus (LMBV) is a major viral pathogen in largemouth bass culture, usually causing high mortality and heavy economic losses. Accurate and early detection of LMBV is crucial for diagnosis and control of the diseases caused by LMBV. Previously, we selected the specific aptamers, LA38 and LA13, targeting LMBV by systematic evolution of ligands by exponential enrichment (SELEX). In this study, we further generated truncated LA38 and LA13 (named as LA38s and LA13s) with high specificity and affinities and developed an aptamer-based sandwich enzyme-linked apta-sorbent assay (ELASA) for LMBV diagnosis. The sandwich ELASA showed high specificity and sensitivity for the LMBV detection, without cross reaction with other viruses. The detection limit of the ELASA was as low as 1.25 \u00d7 102 LMBV-infected cells, and the incubation time of the lysate and biotin labeled aptamer was as short as 10 min. The ELASA could still detect LMBV infection in spleen lysates at dilutions of 1\/25, with good consistency of qRT-PCR. For the fish samples collected from the field, the sensitivity of ELASA was 13.3% less than PCR, but the ELASA was much more convenient and less time consuming. The procedure of ELASA mainly requires washing and incubation, with completion in approximately 4 h. The sandwich ELASA offers a useful tool to rapidly detect LMBV rapidly, contributing to control and prevention of LMBV infection.<\/jats:p>","DOI":"10.3390\/v14050945","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T08:21:25Z","timestamp":1651652485000},"page":"945","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["A Novel Sandwich ELASA Based on Aptamer for Detection of Largemouth Bass Virus (LMBV)"],"prefix":"10.3390","volume":"14","author":[{"given":"Xinyue","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zemiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junrong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingguang","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfeng","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhi","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowen","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1188-9023","authenticated-orcid":false,"given":"Qiwei","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"},{"name":"Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.fsi.2020.10.018","article-title":"Protective immunity of largemouth bass immunized with immersed DNA vaccine against largemouth bass ulcerative syndrome virus","volume":"107","author":"Jia","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"960","DOI":"10.3389\/fgene.2019.00960","article-title":"High-Density Linkage Map and Mapping for Sex and Growth-Related Traits of Largemouth Bass (Micropterus salmoides)","volume":"10","author":"Dong","year":"2019","journal-title":"Front. 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