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The assay was used for laboratory-bred Mastomys rodents, as well as for animals caught in the wild in various regions of West Africa. The ELISA reached an accuracy of 97.1% in samples of known exposure, and a comparison to an immunofluorescence assay (IFA) revealed a very strong agreement between the ELISA and IFA results (Cohen\u2019s kappa of 0.81). The agreement is valid in Nigeria, and in Guinea and Sierra Leone where the lineages II and IV are circulating, respectively. Altogether, these results indicate that this capture ELISA is suitable for LASV IgG serostatus determination in Mastomys rodents as an alternative to IFA. This assay will be a strong, accurate, and semi-quantitative alternative for rodent seroprevalence studies that does not depend on biosafety level 4 infrastructures, providing great benefits for ecology and epidemiology studies of Lassa fever, a disease listed on the Research and Development Blueprint of the WHO.<\/jats:p>","DOI":"10.3390\/v14050993","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T23:27:25Z","timestamp":1652052445000},"page":"993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay"],"prefix":"10.3390","volume":"14","author":[{"given":"Hugo","family":"Soubrier","sequence":"first","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8789-3187","authenticated-orcid":false,"given":"Umaru","family":"Bangura","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5606-7234","authenticated-orcid":false,"given":"Chris","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayodeji","family":"Olayemi","sequence":"additional","affiliation":[{"name":"Natural History Museum, Obafemi Awolowo University, Ile Ife HO220005, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adetunji Samuel","family":"Adesina","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile Ife HO220005, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6562-0230","authenticated-orcid":false,"given":"Stephan","family":"G\u00fcnther","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"},{"name":"German Center for Infectious Diseases (DZIF), Partner Site Hamburg-L\u00fcbeck-Borstel-Riems, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Oestereich","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"},{"name":"German Center for Infectious Diseases (DZIF), Partner Site Hamburg-L\u00fcbeck-Borstel-Riems, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Fichet-Calvet","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"670","DOI":"10.4269\/ajtmh.1970.19.670","article-title":"Lassa fever, a new virus disease of man from West Africa","volume":"19","author":"Frame","year":"1970","journal-title":"Am. 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