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The present study aimed to evaluate the serum levels of GDF-3 and inflammasome-related markers in pregnant women during acute ZIKV infection. Serum samples from pregnant (n = 18) and non-pregnant (n = 22) women with acute ZIKV infection were assessed for NLRP3, IL-1\u03b2, IL-18, and GDF3 markers through an enzyme-linked immunosorbent assay. ZIKV-negative pregnant (n = 18) and non-pregnant women (n = 15) were used as control groups. All serum markers were highly elevated in the ZIKV-infected groups in comparison with control groups (p &lt; 0.0001). Among the ZIKV-infected groups, the serum markers were significantly augmented in the pregnant women in comparison with non-pregnant women (NLRP3 p &lt; 0.001; IL-1\u03b2, IL-18, and GDF3 p &lt; 0.0001). The IL-18 marker was found at significantly higher levels (p &lt; 0.05) in the third trimester of pregnancy. Bivariate and multivariate analyses showed a strong positive correlation between GDF3 and NLRP3 markers among ZIKV-infected pregnant women (r = 0.91, p &lt; 0.0001). The findings indicated that acute ZIKV infection during pregnancy induces the overexpression of GDF-3 and inflammasome-related markers, which may contribute to congenital disorders and harmful pregnancy outcomes.<\/jats:p>","DOI":"10.3390\/v14051004","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T23:39:36Z","timestamp":1652225976000},"page":"1004","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Increased Serum Levels of Growth-Differentiation Factor 3 (GDF3) and Inflammasome-Related Markers in Pregnant Women during Acute Zika Virus Infection"],"prefix":"10.3390","volume":"14","author":[{"given":"Carlos Eduardo","family":"de Castro Alves","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada\u2014PPGIBA, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Amazonas\u2014UFAM, Manaus 69080-900, Brazil"},{"name":"Laborat\u00f3rio de Virologia e Imunologia, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69060-001, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina Ara\u00fajo","family":"de Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia e Imunologia, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69060-001, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"de Melo Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada\u2014PPGIBA, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Amazonas\u2014UFAM, Manaus 69080-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Calheiros","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia e Imunologia, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69060-001, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valdinete Alves do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia de Doen\u00e7as Transmiss\u00edveis na Amaz\u00f4nia, Instituto Le\u00f4nidas e Maria Deane, Fiocruz, Manaus 69057-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Hugo Abdalla","family":"Santos","sequence":"additional","affiliation":[{"name":"Hospital Adventista de Manaus, Manaus 69075-351, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2888-1060","authenticated-orcid":false,"given":"Felipe Gomes","family":"Naveca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia de Doen\u00e7as Transmiss\u00edveis na Amaz\u00f4nia, Instituto Le\u00f4nidas e Maria Deane, Fiocruz, Manaus 69057-070, Brazil"},{"name":"Laborat\u00f3rio de Flaviv\u00edrus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gemilson Soares","family":"Pontes","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada\u2014PPGIBA, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Amazonas\u2014UFAM, Manaus 69080-900, Brazil"},{"name":"Laborat\u00f3rio de Virologia e Imunologia, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69060-001, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.virol.2020.01.015","article-title":"Zika Virus-Spread, Epidemiology, Genome, Transmission Cycle, Clinical Manifestation, Associated Challenges, Vaccine and Antiviral Drug Development","volume":"543","author":"Pielnaa","year":"2020","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.bjid.2020.08.005","article-title":"Clinical and Laboratory Findings of Acute Zika Virus Infection in Patients from Salvador during the First Brazilian Epidemic","volume":"24","author":"Bandeira","year":"2020","journal-title":"Braz. 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