{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:11:46Z","timestamp":1788923506187,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T00:00:00Z","timestamp":1652745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Walloon Public Service, BIOWIN (Health Cluster of Wallonia, Belgium) project: Inteliphages"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Antibiotic resistance represents a major public health concern requiring new alternatives including phage therapy. Klebsiella pneumoniae belongs to the ESKAPE bacteria and can cause urinary tract infections (UTIs). The aims of this study were to isolate and characterize new bacteriophages against a K. pneumoniae strain isolated from UTIs and to assess their efficacy in vitro and in vivo in a Galleria (G.) mellonella larvae model. For this purpose, two bacteriophages were newly isolated against an ST13 K. pneumoniae strain isolated from a UTI and identified as K3 capsular types by wzi gene PCR. Genomic analysis showed that these bacteriophages, named vB_KpnP_K3-ULINTkp1 and vB_KpnP_K3-ULINTkp2, belong to the Drulisvirus genus. Bacteriophage vB_KpnP_K3-ULINTkp1 had the narrowest host spectrum (targeting only K3), while vB_KpnP_K3-ULINTkp2 also infected other Klebsiella types. Short adsorption times and latent periods were observed for both bacteriophages. In vivo experiments showed their ability to replicate in G. mellonella larvae and to decrease host bacterial titers. Moreover, both bacteriophages improved the survival of the infected larvae. In conclusion, these two bacteriophages had different in vitro properties and showed in vivo efficacy in a G. mellonella model with a better efficiency for vB_KpnP_K3-ULINTkp2.<\/jats:p>","DOI":"10.3390\/v14051079","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T03:20:43Z","timestamp":1652844043000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["In Vitro and In Vivo Assessments of Two Newly Isolated Bacteriophages against an ST13 Urinary Tract Infection Klebsiella pneumoniae"],"prefix":"10.3390","volume":"14","author":[{"given":"Fanny","family":"Lafor\u00eat","sequence":"first","affiliation":[{"name":"Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Antoine","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bob","family":"Blasdel Reuter","sequence":"additional","affiliation":[{"name":"V\u00e9sale Bioscience, V\u00e9sale Pharmaceutica, 5310 Noville-sur-Mehaigne, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Detilleux","sequence":"additional","affiliation":[{"name":"Department of Animal Production-Quantitative Genetics, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6863-5219","authenticated-orcid":false,"given":"Jean-Paul","family":"Pirnay","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, 1120 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Brisse","sequence":"additional","affiliation":[{"name":"Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdoulaye","family":"Fall","sequence":"additional","affiliation":[{"name":"Genalyse Partner SA, En Hayeneux 62, 4040 Herstal, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-No\u00ebl","family":"Duprez","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Delcenserie","sequence":"additional","affiliation":[{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-7364","authenticated-orcid":false,"given":"Damien","family":"Thiry","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shaidullina, E., Shelenkov, A., Yanushevich, Y., Mikhaylova, Y., Shagin, D., Alexandrova, I., Ershova, O., Akimkin, V., Kozlov, R., and Edelstein, M. 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