{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:15:18Z","timestamp":1787030118062,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"APQA","award":["B-1543069-2021-22-05"],"award-info":[{"award-number":["B-1543069-2021-22-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens in the swine industry worldwide. In Korea, Fostera PRRS commercial modified live virus (MLV) vaccines have been used since 2014 to control the PRRSV infection. In this study, two PRRSV-2 strains (20D160-1 and 21R2-63-1) were successfully isolated, and their complete genomic sequences were determined. Genetic analysis showed that the two isolates have recombination events between the P129-like strain derived from the Fostera PRRS MLV vaccine and the strain of lineage 1. The 20D160-1 indicated that partial ORF2 to partial ORF4 of the minor parental KNU-1902-like strain, which belongs to Korean lineage C (Kor C) of lineage 1, was inserted into the major parental P129-like strain. The 21R2-63-1 revealed that partial ORF1b of the P129-like strain was inserted into the backbone of the NADC30-like strain. This study is the first to report natural recombinant strains between Fostera PRRS MLV-like strain and the field strain in Korea. These results may have significant implications for MLV evolution and the understanding of PRRSV genetic diversity, while highlighting the need for continuous surveillance of PRRSV.<\/jats:p>","DOI":"10.3390\/v14061153","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T05:25:42Z","timestamp":1653974742000},"page":"1153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Recombination between the Fostera MLV-like Strain and the Strain Belonging to Lineage 1 of Porcine Reproductive and Respiratory Syndrome Virus in Korea"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-1258","authenticated-orcid":false,"given":"Go-Eun","family":"Shin","sequence":"first","affiliation":[{"name":"Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Young","family":"Park","sequence":"additional","affiliation":[{"name":"Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung-Ki","family":"Lee","sequence":"additional","affiliation":[{"name":"Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1892-8910","authenticated-orcid":false,"given":"Bok-Kyung","family":"Ku","sequence":"additional","affiliation":[{"name":"Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choi-Kyu","family":"Park","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Disease Intervention Center, Kyungbuk National University, 80 Daehak-ro, Daegu 41566, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Young","family":"Jeoung","sequence":"additional","affiliation":[{"name":"Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1007\/s00705-015-2672-z","article-title":"Reorganization and expansion of the nidoviral family Arteriviridae","volume":"161","author":"Kuhn","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_2","first-page":"1","article-title":"Reproduction failure of unknown etiology","volume":"1","author":"Keffaber","year":"1989","journal-title":"Am. Assoc. Swine Pract. Newsl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/01652176.1991.9694296","article-title":"Mystery swine disease in The Netherlands: The isolation of Lelystad virus","volume":"13","author":"Wensvoort","year":"1991","journal-title":"Vet. Q."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1002\/9781119350927.ch41","article-title":"Porcine reproductive and respiratory syndrome viruses (porcine arteriviruses)","volume":"Volume 11","author":"Zimmerman","year":"2019","journal-title":"Diseases of Swine"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Guzm\u00e1n, M., Mel\u00e9ndez, R., Jim\u00e9nez, C., Piche, M., Jim\u00e9nez, E., Le\u00f3n, B., Cordero, J.M., Ramirez-Carvajal, L., Uribe, A., and Van Nes, A. (2021). Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-02925-7"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.vetmic.2014.10.007","article-title":"Engineering the PRRS virus genome: Updates and perspectives","volume":"174","author":"Han","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1006\/viro.2001.1034","article-title":"A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b","volume":"287","author":"Wu","year":"2001","journal-title":"Virology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1099\/vir.0.030213-0","article-title":"Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses","volume":"92","author":"Johnson","year":"2011","journal-title":"J. Gen. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/1348-0421.12067","article-title":"Genetic analysis of ORF 5 porcine reproductive and respiratory syndrome virus isolated in Vietnam","volume":"57","author":"Son","year":"2013","journal-title":"Microbiol. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.virusres.2010.08.014","article-title":"Molecular epidemiology of PRRSV: A phylogenetic perspective","volume":"154","author":"Shi","year":"2010","journal-title":"Virus Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1128\/JVI.02551-09","article-title":"Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses","volume":"84","author":"Shi","year":"2010","journal-title":"J. Virol."},{"key":"ref_12","first-page":"77","article-title":"Isolation of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea","volume":"34","author":"Kweon","year":"1994","journal-title":"Korean J. Vet. Res."},{"key":"ref_13","first-page":"363","article-title":"Serological and genetic characterization of the European strain of the porcine reproductive and respiratory syndrome virus isolated in Korea","volume":"46","author":"Kim","year":"2006","journal-title":"Korean J. Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1007\/s00705-021-05169-w","article-title":"Temporal lineage dynamics of the ORF5 gene of porcine reproductive and respiratory syndrome virus in Korea in 2014\u20132019","volume":"166","author":"Kim","year":"2021","journal-title":"Arch. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CVI.00001-15","article-title":"Comparison of two commercial type 1 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against heterologous type 1 and type 2 PRRSV challenge in growing pigs","volume":"22","author":"Kim","year":"2015","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_16","first-page":"57","article-title":"Comparison of 4 commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines against heterologous Korean PRRSV-1 and PRRSV-2 challenge","volume":"83","author":"Oh","year":"2019","journal-title":"Can. J. Vet. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.3201\/eid1512.090390","article-title":"Recombination in vaccine and circulating strains of porcine reproductive and respiratory syndrome viruses","volume":"15","author":"Li","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.virusres.2014.08.005","article-title":"Observation of high recombination occurrence of Porcine Reproductive and Respiratory Syndrome Virus in field condition","volume":"194","author":"Franzo","year":"2014","journal-title":"Virus Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.meegid.2017.07.016","article-title":"A recombinant type 2 porcine reproductive and respiratory syndrome virus between NADC30-like and a MLV-like: Genetic characterization and pathogenicity for piglets","volume":"54","author":"Bian","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.vetmic.2017.04.017","article-title":"Recombination in JXA1-R vaccine and NADC30-like strain of porcine reproductive and respiratory syndrome viruses","volume":"204","author":"Liu","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhou, L., Kang, R., Yu, J., Xie, B., Chen, C., Li, X., Xie, J., Ye, Y., Xiao, L., and Zhang, J. (2018). Genetic characterization and pathogenicity of a novel recombined porcine reproductive and respiratory syndrome virus 2 among Nadc30-like, Jxa1-like, and Mlv-like strains. Viruses, 10.","DOI":"10.3390\/v10100551"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Eclercy, J., Renson, P., Lebret, A., Hirchaud, E., Normand, V., Andraud, M., Paboeuf, F., Blanchard, Y., Rose, N., and Bourry, O. (2019). A field recombinant strain derived from two type 1 porcine reproductive and respiratory syndrome virus (PRRSV-1) modified live vaccines shows increased viremia and transmission in SPF pigs. Viruses, 11.","DOI":"10.3390\/v11030296"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00454-17","DOI":"10.1128\/genomeA.00454-17","article-title":"Complete genome sequence of a recombinant porcine reproductive and respiratory syndrome virus strain from two genotype 1 modified live virus vaccine strains","volume":"5","author":"Renson","year":"2017","journal-title":"Genome Announc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.3201\/eid2512.191111","article-title":"Recombination between vaccine and field strains of porcine reproductive and respiratory syndrome virus","volume":"25","author":"Wang","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1099\/0022-1317-77-6-1271","article-title":"Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the midwestern United States","volume":"77","author":"Kapur","year":"1996","journal-title":"J. Gen. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.virol.2019.01.030","article-title":"Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain","volume":"530","author":"Kwon","year":"2019","journal-title":"Virology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"10904","DOI":"10.1128\/JVI.01270-13","article-title":"Recombination is associated with an outbreak of novel highly pathogenic porcine reproductive and respiratory syndrome viruses in China","volume":"87","author":"Shi","year":"2013","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.vetmic.2014.11.016","article-title":"Re-emerging of porcine respiratory and reproductive syndrome virus (lineage 3) and increased pathogenicity after genomic recombination with vaccine variant","volume":"175","author":"Lu","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/tbed.13067","article-title":"Emergence of novel recombination lineage 3 of porcine reproductive and respiratory syndrome viruses in Southern China","volume":"66","author":"Sun","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virusres.2015.01.014","article-title":"Full genome sequence analysis of a wild, non-MLV-related type 2 Hungarian PRRSV variant isolated in Europe","volume":"200","author":"Balka","year":"2015","journal-title":"Virus Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Evans, A.B., Loyd, H., Dunkelberger, J.R., Van Tol, S., Bolton, M.J., Dorman, K.S., Dekkers, J.C.M., and Carpenter, S. (2017). Antigenic and biological characterization of ORF2-6 variants at early times following PRRSV infection. Viruses, 9.","DOI":"10.3390\/v9050113"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kang, H., Yu, J.E., Shin, J.-E., Kang, A., Kim, W.-I., Lee, C., Lee, J., Cho, I.-S., Choe, S.-E., and Cha, S.-H. (2018). Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1480-6"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1128\/JVI.73.1.152-160.1999","article-title":"Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination","volume":"73","author":"Lole","year":"1999","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"vev003","DOI":"10.1093\/ve\/vev003","article-title":"RDP4: Detection and analysis of recombination patterns in virus genomes","volume":"1","author":"Martin","year":"2015","journal-title":"Virus Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.vetmic.2006.05.007","article-title":"Molecular characterization of recent Korean porcine reproductive and respiratory syndrome (PRRS) viruses and comparison to other Asian PRRS viruses","volume":"117","author":"Cha","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_37","unstructured":"Magstadt, D.R. (2015). Cross-Protection in Fostera\u2122 PRRS Vaccinated Nursery Swine Against Contemporary, Heterologous Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Field Isolates from Different Lineages. [Ph.D. Thesis, Lowa State University]."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.meegid.2015.11.001","article-title":"Genetic diversity of the Korean field strains of porcine reproductive and respiratory syndrome virus","volume":"40","author":"Lee","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hong, S., Wei, Y., Lin, S., Huang, J., He, W., Yao, J., Chen, Y., Kang, O., Huang, W., and Wei, Z. (2019). Genetic analysis of porcine productive and respiratory syndrome virus between 2013 and 2014 in Southern parts of China: Identification of several novel strains with amino acid deletions or insertions in nsp2. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-1906-9"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.virusres.2012.07.030","article-title":"Genomic sequence and virulence comparison of four Type 2 porcine reproductive and respiratory syndrome virus strains","volume":"169","author":"Brockmeier","year":"2012","journal-title":"Virus Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.vetmic.2014.02.027","article-title":"Genomic analysis and pathogenic characteristics of type 2 porcine reproductive and respiratory syndrome virus nsp2 deletion strains isolated in Korea","volume":"170","author":"Choi","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.1007\/s00705-020-04743-y","article-title":"Novel lineage 1 recombinants of porcine reproductive and respiratory syndrome virus isolated from vaccinated herds: Genome sequences and cytokine production profiles","volume":"165","author":"Park","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e01813-19","DOI":"10.1128\/JVI.01813-19","article-title":"Phylogenetics, genomic recombination, and NSP2 polymorphic patterns of porcine reproductive and respiratory syndrome virus in China and the United States in 2014\u20132018","volume":"94","author":"Yu","year":"2020","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"12056","DOI":"10.1073\/pnas.96.21.12056","article-title":"Arterivirus discontinuous mRNA transcription is guided by base pairing between sense and antisense transcription-regulating sequences","volume":"96","author":"Dobbe","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.virol.2015.02.012","article-title":"PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity","volume":"479","author":"Kappes","year":"2015","journal-title":"Virology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-2438-7_1","article-title":"Coronaviruses: An overview of their replication and pathogenesis","volume":"1282","author":"Fehr","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.tim.2016.03.003","article-title":"Epidemiology, genetic recombination, and pathogenesis of coronaviruses","volume":"24","author":"Su","year":"2016","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10712","DOI":"10.1128\/JVI.01446-15","article-title":"Importation and recombination are responsible for the latest emergence of highly pathogenic porcine reproductive and respiratory syndrome virus in China","volume":"89","author":"Zhao","year":"2015","journal-title":"J. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1007\/s11262-020-01751-7","article-title":"Evolutionary and recombination analysis of porcine reproductive and respiratory syndrome isolates in China","volume":"56","author":"Zhang","year":"2020","journal-title":"Virus Genes"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2174\/1874357901711010059","article-title":"NADC30-Like Porcine Reproductive and Respiratory Syndrome in China","volume":"11","author":"Tian","year":"2017","journal-title":"Open Virol. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.meegid.2018.12.006","article-title":"Recombination in lineage 1, 3, 5 and 8 of porcine reproductive and respiratory syndrome viruses in China","volume":"68","author":"Liu","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.meegid.2018.09.024","article-title":"Evolutionary analysis of six isolates of porcine reproductive and respiratory syndrome virus from a single pig farm: MLV-evolved and recombinant viruses","volume":"66","author":"Zhang","year":"2018","journal-title":"Infect. Genet. Evol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1153\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:19:16Z","timestamp":1760138356000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,26]]},"references-count":52,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["v14061153"],"URL":"https:\/\/doi.org\/10.3390\/v14061153","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,26]]}}}