{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T05:23:50Z","timestamp":1761110630078,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"Public Health Service from the National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["P01 AG032958","P51 OD011104"],"award-info":[{"award-number":["P01 AG032958","P51 OD011104"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Center for Research Resources","award":["P01 AG032958","P51 OD011104"],"award-info":[{"award-number":["P01 AG032958","P51 OD011104"]}]},{"DOI":"10.13039\/100000002","name":"Office of Research Infrastructure Programs (ORIP) of the NIH to the Tulane National Primate Research Center","doi-asserted-by":"publisher","award":["P01 AG032958","P51 OD011104"],"award-info":[{"award-number":["P01 AG032958","P51 OD011104"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Primary simian varicella virus (SVV) infection and reactivation in nonhuman primates is a valuable animal model in the study of varicella zoster virus disease [varicella (chickenpox) and herpes zoster (shingles)]. To understand SVV pathogenesis in skin, we inoculated 10 rhesus macaques with SVV, resulting in varicella rash. After the establishment of latency, eight of the monkeys were immunosuppressed using tacrolimus with or without irradiation and prednisone and two monkeys were not immunosuppressed. Zoster rash developed in all immunosuppressed monkeys and in one non-immunosuppressed monkey. Five monkeys had recurrent zoster. During varicella and zoster, SVV DNA in skin scrapings ranged from 50 to 107 copies\/100 ng of total DNA and 2\u2013127 copies\/100 ng of total DNA, respectively. Detection of SVV DNA in blood during varicella was more frequent and abundant compared to that of zoster. During varicella and zoster, SVV antigens colocalized with neurons expressing \u03b2-III tubulin in epidermis, hair follicles, and sweat glands, suggesting axonal transport of the virus. Together, we have demonstrated that both SVV DNA and antigens can be detected in skin lesions during varicella and zoster, providing the basis for further studies on SVV skin pathogenesis, including immune responses and mechanisms of peripheral spread.<\/jats:p>","DOI":"10.3390\/v14061167","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T05:25:42Z","timestamp":1653974742000},"page":"1167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simian Varicella Virus Pathogenesis in Skin during Varicella and Zoster"],"prefix":"10.3390","volume":"14","author":[{"given":"Ravi","family":"Mahalingam","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittany","family":"Feia","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Coleman","sequence":"additional","affiliation":[{"name":"Cell and Developmental Biology Program, School of Medicine, Oregon Health Sciences University, Portland, OR 97201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kusala","family":"Anupindi","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, 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Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen","family":"de Haro","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Doyle-Meyers","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Tulane National Primate Research Center, Tulane University, Covington, LA 70433, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7296-4413","authenticated-orcid":false,"given":"Jayme","family":"Looper","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, LA 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew 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Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1167\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:20:18Z","timestamp":1760138418000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,27]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["v14061167"],"URL":"https:\/\/doi.org\/10.3390\/v14061167","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2022,5,27]]}}}