{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:57:21Z","timestamp":1781625441672,"version":"3.54.5"},"reference-count":68,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Proteus mirabilis is frequently associated with complicated urinary tract infections (UTIs) and is the main cause of catheter-associated urinary tract infections (CAUTIs). Treatment of such infections is complicated and challenging due to the biofilm forming abilities of P. mirabilis. If neglected or mistreated, infections may lead to life-threating conditions such as cystitis, pyelonephritis, kidney failure, and bacteremia that may progress to urosepsis. Treatment with antibiotics, especially in cases of recurring and persistent infections, leads to the development of resistant strains. Recent insights into phage therapy and using phages to coat catheters have been evaluated with many studies showing promising results. Here, we describe a highly lytic bacteriophage, Proteus_virus_309 (41,740 bp), isolated from a wastewater treatment facility in Cape Town, South Africa. According to guidelines of the International Committee on Taxonomy of Viruses (ICTV), bacteriophage 309 is a species within the genus Novosibovirus. Similar to most members of the genus, bacteriophage 309 is strain-specific and lyse P. mirabilis in less than 20 min.<\/jats:p>","DOI":"10.3390\/v14061309","type":"journal-article","created":{"date-parts":[[2022,6,19]],"date-time":"2022-06-19T22:54:52Z","timestamp":1655679292000},"page":"1309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Isolation and Characterization of Lytic Proteus Virus 309"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8974-7535","authenticated-orcid":false,"given":"Joshua","family":"Aaron","sequence":"first","affiliation":[{"name":"Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-8671","authenticated-orcid":false,"given":"Leonardo J.","family":"van Zyl","sequence":"additional","affiliation":[{"name":"Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Bellville 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5157-9046","authenticated-orcid":false,"given":"Leon M. T.","family":"Dicks","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1128\/JB.02136-12","article-title":"Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess l-glutamine","volume":"195","author":"Armbruster","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4027","DOI":"10.1128\/JB.01981-07","article-title":"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility","volume":"190","author":"Pearson","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7294","DOI":"10.1128\/IAI.72.12.7294-7305.2004","article-title":"Mannose-resistant Proteus-like fimbriae are produced by most Proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation","volume":"72","author":"Jansen","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-695X.2007.00284.x","article-title":"Fimbriae of uropathogenic Proteus mirabilis","volume":"51","author":"Rocha","year":"2007","journal-title":"FEMS Microbiol. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Armbruster, C.E., Mobley, H.L.T., and Pearson, M.M. (2018). Pathogenesis of Proteus mirabilis infection. EcoSal Plus., 8.","DOI":"10.1128\/ecosalplus.esp-0009-2017"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2998","DOI":"10.1128\/iai.62.7.2998-3003.1994","article-title":"Unique ability of the Proteus mirabilis capsule to enhance mineral growth in infectious urinary calculi","volume":"62","author":"Dumanski","year":"1994","journal-title":"Infect. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00203-016-1249-x","article-title":"The role of Proteus mirabilis cell wall features in biofilm formation","volume":"198","author":"Czerwonka","year":"2016","journal-title":"Arch. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1128\/AAC.47.2.665-669.2003","article-title":"AcrAB multidrug efflux pump is associated with reduced levels of susceptibility to tigecycline (GAR-936) in Proteus mirabilis","volume":"47","author":"Visalli","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"440","DOI":"10.3389\/fcimb.2021.691210","article-title":"Recurrent urinary tract infection: A mystery in search of better model systems","volume":"11","author":"Murray","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1128\/CMR.00019-07","article-title":"Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis","volume":"21","author":"Jacobsen","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1086\/647371","article-title":"Selected aspects of the socioeconomic impact of nosocomial infections: Morbidity, mortality, cost, and prevention","volume":"17","author":"Jarvis","year":"1996","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1179\/joc.2003.15.1.12","article-title":"Natural antibiotic susceptibility of Proteus spp., with special reference to P. mirabilis and P. penneri strains","volume":"15","author":"Stock","year":"2013","journal-title":"J. Chemother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.4065\/mcp.2011.0411","article-title":"Nitrofurantoin: Preferred empiric therapy for community-acquired lower urinary tract infections","volume":"86","author":"Cunha","year":"2011","journal-title":"Mayo Clin. Proc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.ijantimicag.2014.09.004","article-title":"Molecular epidemiology of CTX-M-type extended-spectrum \u03b2-lactamase-producing Proteus mirabilis isolates in Taiwan","volume":"45","author":"Huang","year":"2015","journal-title":"Int. J. Antimicrob. Agents."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3080","DOI":"10.1128\/JCM.00979-08","article-title":"Detection of KPC-2 in a clinical isolate of Proteus mirabilis and first reported description of carbapenemase resistance caused by a KPC \u03b2-lactamase in P. mirabilis","volume":"46","author":"Tibbetts","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Guillard, T., Grillon, A., de Champs, C., Cartier, C., Madoux, J., Ber\u00e7ot, B., Lebreil, A.-L., Lozniewski, A., Riahi, J., and Vernet-Garnier, V. (2014). Mobile insertion cassette elements found in small non-transmissible plasmids in Proteeae may explain qnrD mobilization. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087801"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"256","DOI":"10.3389\/fmicb.2020.00256","article-title":"Genetics of acquired antibiotic resistance genes in Proteus spp.","volume":"11","author":"Girlich","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"JAMA (1933). Commercial aspects of bacteriophage therapy. J. Am. Med. Assoc., 100, 1603\u20131604.","DOI":"10.1001\/jama.1933.02740200037014"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1749-4486.2009.01973.x","article-title":"A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy","volume":"34","author":"Wright","year":"2009","journal-title":"Clin. Otolaryngol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00066-18","DOI":"10.1128\/CMR.00066-18","article-title":"Phage therapy in the postantibiotic era","volume":"32","author":"Altamirano","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2019). Phage-antibiotic combination treatments: Antagonistic impacts of antibiotics on the pharmacodynamics of phage therapy?. Antibiotics, 8.","DOI":"10.3390\/antibiotics8040182"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1111\/tbed.13646","article-title":"Application of phage therapy: Synergistic effect of phage EcSw (\u03a6EcSw) and antibiotic combination towards antibiotic-resistant Escherichia coli","volume":"67","author":"Easwaran","year":"2020","journal-title":"Transbound Emerg. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1128\/AEM.02850-14","article-title":"Burkholderia cepacia complex Phage-Antibiotic Synergy (PAS): Antibiotics stimulate lytic phage activity","volume":"81","author":"Kamal","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Dickey, J., and Perrot, V. (2019). Adjunct phage treatment enhances the effectiveness of low antibiotic concentration against Staphylococcus aureus biofilms in vitro. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0209390"},{"key":"ref_25","first-page":"926","article-title":"Combination therapy of phage VB_KpnM_P-KP2 and gentamicin combats acute pneumonia caused by K47 serotype Klebsiella pneumoniae","volume":"12","author":"Wang","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1007\/s00253-018-8811-1","article-title":"Applications of bacteriophages versus phage enzymes to combat and cure bacterial infections: An ambitious and also a realistic application?","volume":"102","author":"Maciejewska","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Danis-Wlodarczyk, K., Olszak, T., Arabski, M., Wasik, S., Majkowska-Skrobek, G., Augustyniak, D., Gula, G., Briers, Y., Jang, H.B., and Vandenheuvel, D. (2015). Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137015"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1007\/s00705-019-04305-x","article-title":"Elimination of multidrug-resistant Proteus mirabilis biofilms using bacteriophages","volume":"164","author":"Gomaa","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.3389\/fmicb.2016.01024","article-title":"Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections","volume":"7","author":"Melo","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1039\/C7TB01302G","article-title":"Prevention of encrustation and blockage of urinary catheters by Proteus mirabilis via pH-triggered release of bacteriophage","volume":"5","author":"Milo","year":"2017","journal-title":"J. Mater. Chem. B."},{"key":"ref_31","first-page":"13.3.1","article-title":"Isolation of bacteriophages from environmental sources, and creation and functional screening of phage dna libraries","volume":"7","author":"Pelzek","year":"2013","journal-title":"Curr. Proto. Essent. Lab. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"86","DOI":"10.4161\/bact.1.2.15456","article-title":"Enumeration of bacteriophage particles: Comparative analysis of the traditional plaque assay and real-time qPCR- and nanosight-based assays","volume":"1","author":"Anderson","year":"2011","journal-title":"Bacteriophage"},{"key":"ref_33","first-page":"52781","article-title":"An optimized enrichment technique for the isolation of Arthrobacter bacteriophage species from soil sample isolates","volume":"98","author":"Cross","year":"2015","journal-title":"J. Vis. Exp."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/0042-6822(70)90218-7","article-title":"Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification","volume":"40","author":"Yamamoto","year":"1970","journal-title":"Virology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s40168-020-0795-2","article-title":"Uncovering a hidden diversity: Optimized protocols for the extraction of dsDNA bacteriophages from soil","volume":"8","author":"Loessner","year":"2020","journal-title":"Microbiome"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"jkab074","DOI":"10.1093\/g3journal\/jkab074","article-title":"MultiPhATE2: Code for functional annotation and comparison of phage genomes","volume":"11","author":"Zhou","year":"2021","journal-title":"G3 Genes Genom. Genet."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Moraru, C., Varsani, A., and Kropinski, A.M. (2020). VIRIDIC\u2014A novel tool to calculate the intergenomic similarities of prokaryote-infecting viruses. Viruses, 12.","DOI":"10.1101\/2020.07.05.188268"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1093\/bioinformatics\/btab007","article-title":"Clinker & Clustermap.Js: Automatic generation of gene cluster comparison figures","volume":"37","author":"Gilchrist","year":"2021","journal-title":"Bioinform."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Moraru, C. (2021). VirClust\u2013A tool for hierarchical clustering, core gene detection and annotation of (prokaryotic) viruses. BioRxiv.","DOI":"10.1101\/2021.06.14.448304"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e11396","DOI":"10.7717\/peerj.11396","article-title":"Bacphlip: Predicting bacteriophage lifestyle from conserved protein domains","volume":"9","author":"Hockenberry","year":"2021","journal-title":"Peer J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.foodcont.2018.11.034","article-title":"Bacteriophage potential against Vibrio parahaemolyticus biofilms","volume":"98","author":"Yin","year":"2019","journal-title":"Food Control"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14187","DOI":"10.1038\/ncomms14187","article-title":"A cocktail of three virulent bacteriophages prevents Vibrio cholerae infection in animal models","volume":"8","author":"Yen","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"259","DOI":"10.3389\/fmicb.2020.00259","article-title":"Isolation and characterization of the novel phages VB_VpS_BA3 and VB_VpS_CA8 for lysing Vibrio parahaemolyticus","volume":"11","author":"Yang","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1007\/s00705-018-3853-3","article-title":"Isolation and characterization of a group of new Proteus bacteriophages","volume":"163","author":"Morozova","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.3389\/fmicb.2019.01783","article-title":"Genomic and ecogenomic characterization of Proteus mirabilis bacteriophages","volume":"10","author":"Alves","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1159\/000487137","article-title":"Isolation and characterization of a lytic bacteriophage (VB_PmiS-TH) and its application in combination with ampicillin against planktonic and biofilm forms of Proteus mirabilis isolated from urinary tract infection","volume":"28","author":"Yazdi","year":"2018","journal-title":"Microb. Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e10645","DOI":"10.7717\/peerj.10645","article-title":"Characterization and complete genome sequence of Privateer, a highly prolate Proteus mirabilis podophage","volume":"9","author":"Corban","year":"2021","journal-title":"Peer J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.3389\/fmicb.2020.01171","article-title":"Phage therapy in the year 2035","volume":"11","author":"Pirnay","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1038\/s41598-021-81580-2","article-title":"Designing P. aeruginosa synthetic phages with reduced genomes","volume":"11","author":"Pires","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e00926-19","DOI":"10.1128\/IAI.00926-19","article-title":"Fitness trade-offs resulting from bacteriophage resistance potentiate synergistic antibacterial strategies","volume":"88","author":"Mangalea","year":"2020","journal-title":"Infect. Immun."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Seed, K.D., Faruque, S.M., Mekalanos, J.J., Calderwood, S.B., Qadri, F., and Camilli, A. (2012). Phase variable O antigen biosynthetic genes control expression of the major protective antigen and bacteriophage receptor in Vibrio cholerae O1. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002917"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1128\/JB.01550-08","article-title":"Oxygen-limiting conditions enrich for fimbriate cells of uropathogenic Proteus mirabilis and Escherichia coli","volume":"191","author":"Lane","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/nrmicro2315","article-title":"Bacteriophage resistance mechanisms","volume":"8","author":"Labrie","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Rice, C.J., Kelly, S.A., O\u2019brien, S.C., Melaugh, E.M., Ganacias, J.C.B., Chai, Z.H., Gilmore, B.F., and Skvortsov, T. (2021). Novel phage-derived depolymerase with activity against Proteus mirabilis biofilms. Microorganisms, 9.","DOI":"10.3390\/microorganisms9102172"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1007\/s00705-020-04577-8","article-title":"Taxonomy of prokaryotic viruses: 2018-2019 update from the ICTV bacterial and archaeal viruses subcommittee","volume":"165","author":"Adriaenssens","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"8129","DOI":"10.1093\/nar\/gks516","article-title":"Exploring the dna mimicry of the ocr protein of phage T7","volume":"40","author":"Roberts","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5397","DOI":"10.1093\/nar\/gkaa290","article-title":"Phage T7 DNA mimic protein ocr is a potent inhibitor of BREX defence","volume":"48","author":"Isaev","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.1128\/AEM.02229-13","article-title":"Bacteriophage orphan DNA methyltransferases: Insights from their bacterial origin, function, and occurrence","volume":"79","author":"Murphy","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4233","DOI":"10.1128\/AEM.67.9.4233-4241.2001","article-title":"Bacteriophage latent-period evolution as a response to resource availability","volume":"67","author":"Abedon","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2019). Look who\u2019s talking: T-Even phage lysis inhibition, the granddaddy of virus-virus intercellular communication research. Viruses, 11.","DOI":"10.3390\/v11100951"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1099\/mic.0.2007\/014621-0","article-title":"The lytic cassette of mycobacteriophage Ms6 encodes an enzyme with lipolytic activity","volume":"154","author":"Gil","year":"2008","journal-title":"Microbiology"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Gigante, A.M., Hampton, C.M., Dillard, R.S., Gil, F., Catal\u00e3o, M.J., Moniz-Pereira, J., Wright, E.R., and Pimentel, M. (2017). The Ms6 mycolyl-arabinogalactan esterase LysB is essential for an efficient mycobacteriophage-induced lysis. Viruses, 9.","DOI":"10.3390\/v9110343"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2332","DOI":"10.3389\/fmicb.2018.02332","article-title":"Mechanistic pathways for peptidoglycan O-acetylation and de-O-acetylation","volume":"9","author":"Sychantha","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1432-1033.1991.tb15764.x","article-title":"Dependence of lysozyme-catalysed solubilization of Proteus mirabilis peptidoglycan on the extent of O-acetylation","volume":"195","author":"Dupont","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.carres.2015.02.007","article-title":"Common side reactions of the glycosyl donor in chemical glycosylation","volume":"408","author":"Christensen","year":"2015","journal-title":"Carbohydr. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1007\/s00705-016-2944-2","article-title":"Lytic bacteriophage PM16 specific for Proteus mirabilis: A novel member of the genus Phikmvvirus","volume":"161","author":"Morozova","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1128\/jb.174.7.2172-2177.1992","article-title":"Capsule structure of Proteus mirabilis (ATCC 49565)","volume":"174","author":"Beynon","year":"1992","journal-title":"J. Bacteriol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"104881","DOI":"10.1016\/j.meegid.2021.104881","article-title":"CRISPR-Cas systems in Proteus mirabilis","volume":"92","author":"Fallah","year":"2021","journal-title":"Infect. Genet. Evol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1309\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:31:45Z","timestamp":1760139105000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/6\/1309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,15]]},"references-count":68,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["v14061309"],"URL":"https:\/\/doi.org\/10.3390\/v14061309","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,15]]}}}