{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:43:11Z","timestamp":1760229791458,"version":"build-2065373602"},"reference-count":88,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Polish National Science Centre","doi-asserted-by":"publisher","award":["2017\/25\/B\/NZ9\/01715"],"award-info":[{"award-number":["2017\/25\/B\/NZ9\/01715"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Tomato-infecting viruses have been considered as a serious threat to tomato crops in Poland. Therefore, during 2014\u20132021, 234 tomato samples delivered directly by greenhouse tomato growers to Plant Disease Clinic of IPP-NRI were tested. Eight virus species: pepino mosaic virus (PepMV), tomato yellow ring orthotospovirus (TYRV), tomato spotted wilt orthotospovirus (TSWV), potato virus Y (PVY), cucumber mosaic virus (CMV), tomato black ring virus (TBRV) and tomato mosaic virus (ToMV) were detected in single or mixed infection in 89 samples. The presence of TYRV was established for the first time in Poland in 2014. Since then, its presence has been observed in single and mixed infection with TSWV and CMV. Here, we analysed the genetic variability of TYRV population based on complete nucleocapsid (N) protein gene sequence of 55 TYRV isolates. Maximum-likelihood reconstruction revealed the presence of three distinct, well-supported phylogroups. Moreover, the effect of host species on virus diversity was confirmed. Therefore, RT-LAMP assay was developed for the rapid and efficient detection of TYRV isolates that can be implemented in field and greenhouse conditions.<\/jats:p>","DOI":"10.3390\/v14071405","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:07:02Z","timestamp":1656374822000},"page":"1405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence, Genetic Variability of Tomato Yellow Ring Orthotospovirus Population and the Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Its Rapid Detection"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4944-2910","authenticated-orcid":false,"given":"Aleksandra","family":"Zarzy\u0144ska-Nowak","sequence":"first","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3923-0829","authenticated-orcid":false,"given":"Daria","family":"Budzy\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3059-3041","authenticated-orcid":false,"given":"Agnieszka","family":"Taberska","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"J\u0119drzejczak","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7773-9079","authenticated-orcid":false,"given":"Julia","family":"Minicka","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4217-6421","authenticated-orcid":false,"given":"Natasza","family":"Borodynko-Filas","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8267-023X","authenticated-orcid":false,"given":"Beata","family":"Hasi\u00f3w-Jaroszewska","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection-National Research Institute, ul. W\u0142. W\u0119gorka 20, 60-318 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"ref_1","unstructured":"(2022, March 17). Food and Agriculture Organization of the United Nations. Available online: https:\/\/www.fao.org\/home\/en."},{"key":"ref_2","unstructured":"(2022, April 25). Statistics|Eurostat. Available online: https:\/\/ec.europa.eu\/eurostat\/databrowser\/view\/tag00115\/default\/table?lang=en."},{"key":"ref_3","unstructured":"(2022, April 25). 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