{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:07:31Z","timestamp":1791871651188,"version":"4.3.4"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia de Pernambuco\/FACEPE","award":["APQ-0055.2.11\/16"],"award-info":[{"award-number":["APQ-0055.2.11\/16"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia de Pernambuco\/FACEPE","award":["APQ-0044.2.11\/16"],"award-info":[{"award-number":["APQ-0044.2.11\/16"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia de Pernambuco\/FACEPE","award":["#400763\/2019-2"],"award-info":[{"award-number":["#400763\/2019-2"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia de Pernambuco\/FACEPE","award":["307887\/2019-7"],"award-info":[{"award-number":["307887\/2019-7"]}]},{"name":"JBS\u2014Fazer o Bem faz Bem","award":["APQ-0055.2.11\/16"],"award-info":[{"award-number":["APQ-0055.2.11\/16"]}]},{"name":"JBS\u2014Fazer o Bem faz Bem","award":["APQ-0044.2.11\/16"],"award-info":[{"award-number":["APQ-0044.2.11\/16"]}]},{"name":"JBS\u2014Fazer o Bem faz Bem","award":["#400763\/2019-2"],"award-info":[{"award-number":["#400763\/2019-2"]}]},{"name":"JBS\u2014Fazer o Bem faz Bem","award":["307887\/2019-7"],"award-info":[{"award-number":["307887\/2019-7"]}]},{"name":"CNPq Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["APQ-0055.2.11\/16"],"award-info":[{"award-number":["APQ-0055.2.11\/16"]}]},{"name":"CNPq Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["APQ-0044.2.11\/16"],"award-info":[{"award-number":["APQ-0044.2.11\/16"]}]},{"name":"CNPq Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["#400763\/2019-2"],"award-info":[{"award-number":["#400763\/2019-2"]}]},{"name":"CNPq Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["307887\/2019-7"],"award-info":[{"award-number":["307887\/2019-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Newcastle disease virus (NDV), also known as avian paramyxoviruses 1 (APMV-1) is among the most important viruses infecting avian species. Given its widespread circulation, there is a high risk for the reintroduction of virulent strains into the domestic poultry industry, making the surveillance of wild and domestic birds a crucial process to appropriately respond to novel outbreaks. In the present study, we investigated an outbreak characterized by the identification of sick pigeons in a large municipality in Northeastern Brazil in 2018. The affected pigeons presented neurological signs, including motor incoordination, torticollis, and lethargy. Moribund birds were collected, and through a detailed histopathological analysis we identified severe lymphoplasmacytic meningoencephalitis with perivascular cuffs and gliosis in the central nervous system, and lymphoplasmacytic inflammation in the liver, kidney, and intestine. A total of five pigeons tested positive for NDV, as assessed by rRT-PCR targeted to the M gene. Laboratory virus isolation on Vero E6 cells confirmed infection, after the recovery of infectious NVD from brain and kidney tissues. We next characterized the isolated NDV\/pigeon\/PE-Brazil\/MP003\/2018 by next-generation sequencing (NGS). Phylogenetic analysis grouped the virus with other NDV class II isolates from subgenotype VI.2.1.2, including two previous NDV isolates from Brazil in 2014 and 2019. The diversity of aminoacid residues at the fusion F protein cleavage site was analyzed identifying the motif RRQKR\u2193F, typical of virulent strains. Our results all highlight the importance of virus surveillance in wild and domestic birds, especially given the risk of zoonotic NDV.<\/jats:p>","DOI":"10.3390\/v14071579","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T03:58:44Z","timestamp":1658462324000},"page":"1579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification of a Virulent Newcastle Disease Virus Strain Isolated from Pigeons (Columbia livia) in Northeastern Brazil Using Next-Generation Genome Sequencing"],"prefix":"10.3390","volume":"14","author":[{"given":"Mylena Ribeiro","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"},{"name":"Department of Virology and Experimental Therapy, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lais Ceschini","family":"Machado","sequence":"additional","affiliation":[{"name":"Department of Entomology, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Dias","family":"de Oliveira Carvalho","sequence":"additional","affiliation":[{"name":"Department of Entomology, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaise Yasmine Vasconcelos","family":"de Lima Cavalcanti","sequence":"additional","affiliation":[{"name":"Department of Virology and Experimental Therapy, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4077-4329","authenticated-orcid":false,"given":"Givaldo Bom","family":"da Silva Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9346-2957","authenticated-orcid":false,"given":"Telma","family":"de Sousa Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8923-4473","authenticated-orcid":false,"given":"Silvio Miguel Castillo","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6770-8797","authenticated-orcid":false,"given":"Francisco","family":"de Assis Leite Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1419-5713","authenticated-orcid":false,"given":"Gabriel","family":"da Luz Wallau","sequence":"additional","affiliation":[{"name":"Department of Entomology, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5641-0883","authenticated-orcid":false,"given":"F\u00e1bio","family":"de Souza Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Diagnosis, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1846-6007","authenticated-orcid":false,"given":"Rafael Freitas","family":"de Oliveira Franca","sequence":"additional","affiliation":[{"name":"Department of Virology and Experimental Therapy, Oswaldo Cruz Foundation\u2014Fiocruz, Recife 54740-465, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1007\/s00705-019-04247-4","article-title":"Taxonomy of the order Mononegavirales: Update 2019","volume":"164","author":"Amarasinghe","year":"2019","journal-title":"Arch. 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