{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:10:57Z","timestamp":1781007057307,"version":"3.54.1"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T00:00:00Z","timestamp":1658707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Introducing Talents Scientific Research Project of Inner Mongolia Agricultural University","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]},{"name":"Inner Mongolia Natural Science Foundation \u2018Development of Marc-145 cells stable expressing PRRSV ORF2\/ORF4 and replication-defective vaccine candidate strain\u2019","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Inner Mongolia Natural Science Foundation \u2018Development of Marc-145 cells stable expressing PRRSV ORF2\/ORF4 and replication-defective vaccine candidate strain\u2019","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Inner Mongolia Natural Science Foundation \u2018Development of Marc-145 cells stable expressing PRRSV ORF2\/ORF4 and replication-defective vaccine candidate strain\u2019","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]},{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, PR China","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, PR China","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, PR China","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]},{"name":"Ruminant Animal Disease Diagnosis center, Inner Mongolia Agricultural University","award":["NDGCC2016-22"],"award-info":[{"award-number":["NDGCC2016-22"]}]},{"name":"Ruminant Animal Disease Diagnosis center, Inner Mongolia Agricultural University","award":["NDYB2018-2"],"award-info":[{"award-number":["NDYB2018-2"]}]},{"name":"Ruminant Animal Disease Diagnosis center, Inner Mongolia Agricultural University","award":["2020MS03047"],"award-info":[{"award-number":["2020MS03047"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Bovine respiratory disease complex (BRDC) is a comprehensive disease in cattle caused by various viral and bacterial infections. Among them, bovine herpesvirus type I (BoHV\u22121) and bovine viral diarrhea virus (BVDV) play important roles and have caused huge financial losses for the cattle industry worldwide. At present, vaccines against BRDC include trivalent attenuated BoHV\u22121, BVDV\u22121, and BVDV\u22122 live vaccines, BoHV\u22121 live attenuated vaccines, and BoHV\u22121\/BVDV bivalent live attenuated vaccines, which have limitations in terms of their safety and efficacy. To solve these problems, we optimized the codon of the BVDV\u22121 E2 gene, added the signal peptide sequence of the BoHV\u22121 gD gene, expressed double BVDV\u22121 E2 glycoproteins in tandem at the BoHV\u22121 gE gene site, and constructed a BoHV\u22121 genetics-engineered vectored vaccine with gE gene deletion, named BoHV\u22121 gE\/E2\u2212Linker\u2212E2+ and BoHV\u22121 \u0394gE. This study compared the protective effects in BoHV\u22121, BoHV\u22121 \u0394gE, BoHV\u22121 gE\/E2\u2212Linker\u2212E2+, and BVDV\u22121 inactivated antigen immunized guinea pigs and calves. The results showed that BoHV\u22121 gE\/E2\u2212Linker\u2212E2+ could successfully induce guinea pigs and calves to produce specific neutralizing antibodies against BVDV\u22121. In addition, after BoHV\u22121 and BVDV\u22121 challenges, BoHV\u22121 gE\/E2\u2212Linker\u2212E2+ can produce a specific neutralizing antibody response against BoHV\u22121 and BVDV\u22121 infections. Calves immunized with this type of virus can be distinguished as either vaccinated animals (gE-) or naturally infected animals (gE+). In summary, our data suggest that BoHV\u22121 gE\/E2\u2212Linker\u2212E2+ and BoHV\u22121 \u0394gE have great potential to prevent BVDV\u22121 or BoHV\u22121 infection.<\/jats:p>","DOI":"10.3390\/v14081618","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T00:17:27Z","timestamp":1658794647000},"page":"1618","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses"],"prefix":"10.3390","volume":"14","author":[{"given":"Chun-Yu","family":"Liu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Zhe","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Na","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Jun","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.2527\/jas.2015-9023","article-title":"Evaluation of multiple ancillary therapies used in combination with an antimicrobial in newly received high-risk calves treated for bovine respiratory disease","volume":"93","author":"Wilson","year":"2015","journal-title":"J. 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