{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:01:38Z","timestamp":1789646498940,"version":"4.0.0"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IDEXX Laboratories"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>(1) Background: This study aimed to detect feline coronavirus (FCoV) and characterize spike (S) gene mutation profiles in cats suffering from diseases other than feline infectious peritonitis (FIP) using commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) and reevaluating results by sequencing. (2) Methods: In 87 cats in which FIP was excluded by histopathology and immunohistochemistry, FCoV 7b gene and S gene mutation RT-qPCR was performed prospectively on incisional biopsies and fine-needle aspirates of different organs, body fluids, and feces. Samples positive for S gene mutations or mixed FCoV underwent sequencing. (3) Results: In 21\/87 cats, FCoV RNA was detectable. S gene mutations were detected by commercial RT-qPCR (and a diagnostic algorithm that was used at the time of sample submission) in at least one sample in 14\/21 cats (66.7%), with only mutated FCoV in 2\/21, only mixed in 1\/21, and different results in 11\/21 cats; in the remaining 7\/21 cats, RNA load was too low to differentiate. However, sequencing of 8 tissue samples and 8 fecal samples of 9 cats did not confirm mutated FCoV in any of the FCoV RNA-positive cats without FIP. (4) Conclusions: Sequencing results did not confirm results of the commercial S gene mutation RT-qPCR.<\/jats:p>","DOI":"10.3390\/v14081671","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T23:49:27Z","timestamp":1659397767000},"page":"1671","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis"],"prefix":"10.3390","volume":"14","author":[{"given":"St\u00e9phanie","family":"J\u00e4hne","sequence":"first","affiliation":[{"name":"Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Felten","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Bergmann","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Erber","sequence":"additional","affiliation":[{"name":"Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaspar","family":"Matiasek","sequence":"additional","affiliation":[{"name":"Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3609-2416","authenticated-orcid":false,"given":"Marina L.","family":"Meli","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9750-4296","authenticated-orcid":false,"given":"Regina","family":"Hofmann-Lehmann","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterin\u00e4rstra\u00dfe 13, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1136\/vr.130.7.133","article-title":"A Study of Naturally Occurring Feline Coronavirus Infections in Kittens","volume":"130","author":"Addie","year":"1992","journal-title":"Vet. 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