{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:51:05Z","timestamp":1790481065889,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T00:00:00Z","timestamp":1659139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The aim of this study is to follow the gp production in IBDV-vaccinated and challenged birds. The progress of IBDV infection was monitored using anti-VP2 immunocytochemistry, light and transmission electron microscopy. In the medulla of the bursal follicle, the Movat pentachrome staining discovered an extracellular glycoprotein (gp) produced by bursal secretory dendritic cells (BSDCs). The secretory granules of BSDCs either discharge resulting in extracellular gp or fuse together forming intracellular corpuscles. The double fate of granules suggests a dual function of BSDCs: (a.) For the discharged granules, gp contributes to the medullary microenvironment (ME). (b.) The intracellular corpuscles may be the sign of BSDC transformation to a macrophage-like cell (Mal). Infectious bursal disease virus (IBDV) infection accelerates the BSDC transformation to Mal. The decreased number of BSDCs is feedback for the precursor cells of BSDCs lodging in the cortico-medullary epithelial arches (CMEA), where they proliferate. Opening the CMEA, the precursor cells enter the medulla, and differentiate to immature BSDCs. The virus uptake in the corpuscles prevents the granular discharge resulting in the absence of gp and alteration in ME. In vaccine-take birds, the mitotic rate of BSDC precursor cells cannot restore the precursor pool; therefore, in the case of IBDV challenge, the number of newly formed BSDCs is too low for outbreak of clinical disease. The BSDCs, as a primary target of IBDV, may contribute to the long-lasting immunosuppressive status of IBDV-infected chickens.<\/jats:p>","DOI":"10.3390\/v14081689","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T23:49:27Z","timestamp":1659397767000},"page":"1689","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens"],"prefix":"10.3390","volume":"14","author":[{"given":"Bal\u00e1zs","family":"Felf\u00f6ldi","sequence":"first","affiliation":[{"name":"Ceva-Phylaxia Ltd., 1107 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zs\u00f3fia","family":"Benyeda","sequence":"additional","affiliation":[{"name":"Biovo Animal Health Ltd., 7700 Moh\u00e1cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e1ndor","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Magyar","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imre","family":"Ol\u00e1h","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology Semmelweis University, 1094 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1080\/03079459708419254","article-title":"Comparison of the in situ changes in lymphoid cells during infection with infectious bursal disease virus in chickens of different ages","volume":"26","author":"Vervelde","year":"1997","journal-title":"Avian Pathol."},{"key":"ref_2","first-page":"154","article-title":"Immunodeficiency in the chicken. IV. An immunological study of infectious bursal disease","volume":"23","author":"Ivanyi","year":"1976","journal-title":"Clin. Exp. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-3-642-67717-5_5","article-title":"Infectious Bursal Disease Virus","volume":"90","author":"Becht","year":"1980","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1128\/iai.27.2.364-367.1980","article-title":"Significance of bursa of Fabricius as target organ in infectious bursal disease of chickens","volume":"27","author":"Weiss","year":"1980","journal-title":"Infect. Immun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/03079459008418708","article-title":"Pathogenesis of infectious bursal disease in embryonally bursectomised chickens","volume":"19","author":"Okoye","year":"1990","journal-title":"Avian Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0145-305X(91)90029-X","article-title":"The effect of infectious bursal disease virus on B lymphocytes and bursal stromal components in specific pathogen-free (SPF) white leghorn chickens","volume":"15","author":"Ramm","year":"1991","journal-title":"Dev. Comp. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"714","DOI":"10.2307\/1590314","article-title":"Pathogenesis of Infectious Bursal Disease in Chickens Infected with Virus at Various Ages","volume":"27","author":"Fadly","year":"1983","journal-title":"Avian Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1080\/03079459208418882","article-title":"Pathogenesis of infectious bursal disease in cyclophosphamide-treated chickens","volume":"21","author":"Okoye","year":"1992","journal-title":"Avian Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"511","DOI":"10.3382\/ps.0490511","article-title":"Infectivity of Infectious Bursal Disease Virus for Embryonating Eggs","volume":"49","author":"Hitchner","year":"1970","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1637\/0005-2086(2007)051[0014:BEOVAI]2.0.CO;2","article-title":"Bilateral effects of vaccination against infectious bursal disease and Newcastle disease in specific-pathogen-free layers and commercial broiler chickens","volume":"51","author":"Rautenschlein","year":"2007","journal-title":"Avian Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2005.02.011","article-title":"Differences in the immunopathogenesis of infectious bursal disease virus (IBDV) following in ovo and post-hatch vaccination of chickens","volume":"106","author":"Rautenschlein","year":"2005","journal-title":"Veter. Immunol. Immunopathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1080\/03079459108418779","article-title":"Acute infectious bursal disease in poultry: Protection afforded by maternally derived antibodies and interference with live vaccination","volume":"20","author":"Berg","year":"1991","journal-title":"Avian Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"334","DOI":"10.2307\/1589701","article-title":"Response of Mibolerone-Treated Chickens to Infectious Bursal Disease Virus","volume":"24","author":"Lucio","year":"1980","journal-title":"Avian Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"179","DOI":"10.2307\/1589777","article-title":"Immune Response of White Leghorn Chicks from Vaccination with Different Strains of Infectious Bursal Disease Virus and in the Presence of Maternal Antibodies","volume":"24","author":"Winterfield","year":"1980","journal-title":"Avian Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"648","DOI":"10.2307\/1590562","article-title":"Effect of Maternal Antibody on Timing of Initial Vaccination of Young White Leghorn Chickens against Infectious Bursal Disease Virus","volume":"30","author":"Solano","year":"1986","journal-title":"Avian Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/ar.1092190307","article-title":"Bursal secretory cells: An electron microscope study","volume":"219","author":"Olah","year":"1987","journal-title":"Anat. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101000","DOI":"10.1016\/j.psj.2021.01.023","article-title":"Infection of bursal disease virus abrogates the extracellular glycoprotein in the follicular medulla","volume":"100","author":"Benyeda","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101727","DOI":"10.1016\/j.psj.2022.101727","article-title":"The bursal secretory dendritic cell (BSDC) and the enigmatic chB6+ macrophage-like cell (Mal)","volume":"101","author":"Benyeda","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0145-305X(88)90010-9","article-title":"Chicken mucin-cross-reactive antigen","volume":"12","author":"Chechik","year":"1988","journal-title":"Dev. Comp. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1099\/0022-1317-77-6-1229","article-title":"Susceptibility of chicken lymphoid cells to infectious bursal disease virus does not correlate with the presence of specific binding sites","volume":"77","author":"Nieper","year":"1996","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1099\/0022-1317-82-1-159","article-title":"VP5 and the N terminus of VP2 are not responsible for the different pathotype of serotype I and II infectious bursal disease virus","volume":"82","author":"Goovaerts","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1111\/j.1365-2567.2006.02332.x","article-title":"Diversified bursal medullary B cells survive and expand independently after depletion following neonatal infectious bursal disease virus infection","volume":"117","author":"Withers","year":"2006","journal-title":"Immunology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"483","DOI":"10.2307\/1589381","article-title":"Electron-Microscope Studies on the Pathogenesis of Infectious Bursal Disease after Intrabursal Application of the Causal Virus","volume":"20","author":"Weiss","year":"1976","journal-title":"Avian Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.virusres.2005.04.014","article-title":"Infection and activation of bursal macrophages by virulent infectious bursal disease virus","volume":"113","author":"Khatri","year":"2005","journal-title":"Virus Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.dci.2013.03.017","article-title":"Infectious Bursal Disease: A complex host\u2013pathogen interaction","volume":"41","author":"Ingrao","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0042-6822(81)90112-4","article-title":"Hematopoietic cells transformed in vitro by REVT avian reticuloendotheliosis virus express characteristics of very immature lymphoid cells","volume":"115","author":"Beug","year":"1981","journal-title":"Virology"},{"key":"ref_27","unstructured":"Akad\u00e9mia, K., and Budapest, H.J.B. (1975). Ultrostructure of Lymphoid Organs: An Electron-Microscopic Atlas, Masson et Cie."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"108663","DOI":"10.1016\/j.vetmic.2020.108663","article-title":"Occurrence and spread of a reassortant very virulent genotype of infectious bursal disease virus with altered VP2 amino acid profile and pathogenicity in some European countries","volume":"245","author":"Jansson","year":"2020","journal-title":"Veter. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1099\/0022-1317-72-8-1835","article-title":"Sequence Analysis and Expression of the Host-protective Immunogen VP2 of a Variant Strain of Infectious Bursal Disease Virus Which Can Circumvent Vaccination with Standard Type I Strains","volume":"72","author":"Heine","year":"1991","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.1007\/s00705-017-3500-4","article-title":"Classification of infectious bursal disease virus into genogroups","volume":"162","author":"Michel","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_31","unstructured":"Bancroft, J.D., and Gamble, M. (2002). Theory and Practice of Histological Techniques, Churchill Livingstone. [5th ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/ar.1092430313","article-title":"Dendritic cells in the bursal follicles and germinal centers of the chicken\u2019s caecal tonsil express vimentin but not desmin","volume":"243","author":"Glick","year":"1995","journal-title":"Anat. Rec."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/ar.1092330115","article-title":"Differentiation of bursal secretory-dendritic cells studied with anti-vimentin monoclonal antibody","volume":"233","author":"Olah","year":"1992","journal-title":"Anat. Rec."},{"key":"ref_34","first-page":"43","article-title":"The bursal microenvironment: Phenotypic characterization of the epithelial component of the bursa of Fabricius with the use of monoclonal antibodies","volume":"58","author":"Houssaint","year":"1986","journal-title":"Immunology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1002\/ar.1092330412","article-title":"Follicle-associated epithelium and medullary epithelial tissue of the bursa of Fabricius are two different compartments","volume":"233","author":"Olah","year":"1992","journal-title":"Anat. Rec."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/03079450600821141","article-title":"Impact of heterophil granulocyte depletion caused by 5-fluorouracil on infectious bursal disease virus infection in specific pathogen free chickens","volume":"35","author":"Kabell","year":"2006","journal-title":"Avian Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"103839","DOI":"10.1016\/j.dci.2020.103839","article-title":"Very virulent infectious bursal disease virus-induced immune injury is involved in inflammation, apoptosis, and inflammatory cytokines imbalance in the bursa of fabricius","volume":"114","author":"Huang","year":"2020","journal-title":"Dev. Comp. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/jlb.40.4.469","article-title":"The Follicle-Associated Epithelium in the Bursa of Fabricius Cell Origin Studied by Means of Quail-Chick Chimeras and Monoclonal Antibodies","volume":"40","author":"Houssaint","year":"1986","journal-title":"J. Leukoc. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1080\/03079450701589159","article-title":"Pivotal role of ChIFN\u03b3 in the pathogenesis and immunosuppression of infectious bursal disease","volume":"36","author":"Rauw","year":"2007","journal-title":"Avian Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"741","DOI":"10.2307\/1592023","article-title":"Presence of Lesions without Virus Replication in the Thymus of Chickens Exposed to Infectious Bursal Disease Virus","volume":"37","author":"Sharma","year":"1993","journal-title":"Avian Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1006\/viro.2000.0595","article-title":"VP5, the Nonstructural Polypeptide of Infectious Bursal Disease Virus, Accumulates within the Host Plasma Membrane and Induces Cell Lysis","volume":"277","author":"Lombardo","year":"2000","journal-title":"Virology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1006\/viro.1998.9187","article-title":"Infectious Bursal Disease Virus Changes the Potassium Current Properties of Chicken Embryo Fibroblasts","volume":"246","author":"Repp","year":"1998","journal-title":"Virology"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"M\u00e9ndez, F., Romero, N., Cubas, L.L., Delgui, L.R., Rodriguez, D., and Rodr\u00edguez, J.F. (2017). Non-Lytic Egression of Infectious Bursal Disease Virus (IBDV) Particles from Infected Cells. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0170080"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1099\/0022-1317-76-2-437","article-title":"Identification of a novel viral protein in infectious bursal disease virus-infected cells","volume":"76","author":"Mundt","year":"1995","journal-title":"J. Gen. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1689\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:59:57Z","timestamp":1760140797000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,30]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["v14081689"],"URL":"https:\/\/doi.org\/10.3390\/v14081689","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,30]]}}}