{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:13:39Z","timestamp":1787030019941,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Laboratory of the Lingnan Modern Agriculture Project","award":["NT2021010"],"award-info":[{"award-number":["NT2021010"]}]},{"name":"Laboratory of the Lingnan Modern Agriculture Project","award":["31972521"],"award-info":[{"award-number":["31972521"]}]},{"name":"National Natural Science Foundation of China","award":["NT2021010"],"award-info":[{"award-number":["NT2021010"]}]},{"name":"National Natural Science Foundation of China","award":["31972521"],"award-info":[{"award-number":["31972521"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The emergence of multidrug-resistant bacterial pathogens poses a serious global health threat. While patient infections by the opportunistic human pathogen Pseudoxanthomonas spp. have been increasingly reported worldwide, no phage associated with this bacterial genus has yet been isolated and reported. In this study, we isolated and characterized the novel phage PW916 to subsequently be used to lyse the multidrug-resistant Pseudoxanthomonas kaohsiungensi which was isolated from soil samples obtained from Chongqing, China. We studied the morphological features, thermal stability, pH stability, optimal multiplicity of infection, and genomic sequence of phage PW916. Transmission electron microscopy revealed the morphology of PW916 and indicated it to belong to the Siphoviridae family, with the morphological characteristics of a rounded head and a long noncontractile tail. The optimal multiplicity of infection of PW916 was 0.1. Moreover, PW916 was found to be stable under a wide range of temperatures (4\u201360 \u00b0C), pH (4\u201311) as well as treatment with 1% (v\/w) chloroform. The genome of PW916 was determined to be a circular double-stranded structure with a length of 47,760 bp, containing 64 open reading frames that encoded functional and structural proteins, while no antibiotic resistance nor virulence factor genes were detected. The genomic sequencing and phylogenetic tree analysis showed that PW916 was a novel phage belonging to the Siphoviridae family that was closely related to the Stenotrophomonas phage. This is the first study to identify a novel phage infecting the multidrug-resistant P. kaohsiungensi and the findings provide insight into the potential application of PW916 in future phage therapies.<\/jats:p>","DOI":"10.3390\/v14081709","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:15:26Z","timestamp":1659485726000},"page":"1709","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Isolation and Characterization of the Lytic Pseudoxanthomonas kaohsiungensi Phage PW916"],"prefix":"10.3390","volume":"14","author":[{"given":"Chang","family":"Wen","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaofan","family":"Ai","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyun","family":"Lu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiue","family":"Yang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7539-2668","authenticated-orcid":false,"given":"Hanpeng","family":"Liao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shungui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1099\/00207713-50-1-273","article-title":"Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov","volume":"50","author":"Finkmann","year":"2000","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"108145","DOI":"10.1016\/j.dib.2022.108145","article-title":"Genomic data resource of type strains of genus Pseudoxanthomonas","volume":"42","author":"Bansal","year":"2022","journal-title":"Data Brief"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1099\/ijs.0.02810-0","article-title":"Pseudoxanthomonas mexicana sp. nov. and Pseudoxanthomonas japonensis sp. nov., isolated from diverse environments, and emended descriptions of the genus Pseudoxanthomonas Finkmann et al. 2000 and of its type species","volume":"54","author":"Thierry","year":"2004","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.jmii.2017.12.003","article-title":"An oil refinery worker at Kaohsiung, with Pseudoxanthomonas kaohsiungensis bloodstream infection presenting as chronic pericarditis and masquerading as tuberculosis pericarditis","volume":"51","author":"Kuo","year":"2018","journal-title":"J. Microbiol. Immunol. Infect. = Wei Mian Yu Gan Ran Za Zhi"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.3389\/fcimb.2021.724142","article-title":"A Correlation Study of the Microbiota Between Oral Cavity and Tonsils in Children With Tonsillar Hypertrophy","volume":"11","author":"Xu","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6313","DOI":"10.1099\/ijsem.0.004533","article-title":"Pseudoxanthomonas winnipegensis sp. nov., derived from human clinical materials and recovered from cystic fibrosis and other patient types in Canada, and emendation of Pseudoxanthomonas spadix Young et al. 2007","volume":"70","author":"Bernard","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1016\/j.ygeno.2021.09.021","article-title":"Deep phylo-taxono genomics reveals Xylella as a variant lineage of plant associated Xanthomonas and supports their taxonomic reunification along with Stenotrophomonas and Pseudoxanthomonas","volume":"113","author":"Bansal","year":"2021","journal-title":"Genomics"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Clavijo-Coppens, F., Ginet, N., Cesbron, S., Briand, M., Jacques, M.A., and Ansaldi, M. (2021). Novel Virulent Bacteriophages Infecting Mediterranean Isolates of the Plant Pest Xylella fastidiosa and Xanthomonas albilineans. Viruses, 13.","DOI":"10.3390\/v13050725"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41385-019-0250-5","article-title":"New insights into intestinal phages","volume":"13","author":"Sausset","year":"2020","journal-title":"Mucosal Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"fnw047","DOI":"10.1093\/femsle\/fnw047","article-title":"Diversity of phage infection types and associated terminology: The problem with \u201cLytic or lysogenic\u201d","volume":"363","author":"Hobbs","year":"2016","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.copbio.2020.09.016","article-title":"Phages to shape the gut microbiota?","volume":"68","author":"Dahlman","year":"2021","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1128\/br.40.4.793-802.1976","article-title":"Who discovered bacteriophage?","volume":"40","author":"Duckworth","year":"1976","journal-title":"Bacteriol Rev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ganeshan, S.D., and Hosseinidoust, Z. (2019). Phage Therapy with a focus on the Human Microbiota. Antibiotics, 8.","DOI":"10.3390\/antibiotics8030131"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.medmal.2008.06.023","article-title":"Phage therapy experience at the Eliava Institute","volume":"38","author":"Kutateladze","year":"2008","journal-title":"M\u00e9decine Mal. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4161\/bact.1.2.15845","article-title":"Phage treatment of human infections","volume":"1","author":"Abedon","year":"2014","journal-title":"Bacteriophage"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Djebara, S., Maussen, C., De Vos, D., Merabishvili, M., Damanet, B., Pang, K.W., De Leenheer, P., Strachinaru, I., Soentjens, P., and Pirnay, J.P. (2019). Processing Phage Therapy Requests in a Brussels Military Hospital: Lessons Identified. Viruses, 11.","DOI":"10.3390\/v11030265"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"00954-17","DOI":"10.1128\/AAC.00954-17","article-title":"Development and use of personalized bacteriophage-based therapeutic cocktails to treat a patient with a disseminated resistant Acinetobacter baumannii infection","volume":"61","author":"Schooley","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_18","unstructured":"Dedrick, R.M., Smith, B.E., Cristinziano, M., Freeman, K.G., Jacobs-Sera, D., Belessis, Y., Whitney Brown, A., Cohen, K.A., Davidson, R.M., and van Duin, D. (2022). Phage Therapy of Mycobacterium Infections: Compassionate-use of Phages in Twenty Patients with Drug-Resistant Mycobacterial Disease. Clin. Infect. Dis., ciac453."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1038\/s41587-019-0328-3","article-title":"Phage combination therapies for bacterial wilt disease in tomato","volume":"37","author":"Wang","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/j.cell.2019.03.040","article-title":"Marine DNA Viral Macro- and Microdiversity from Pole to Pole","volume":"177","author":"Gregory","year":"2019","journal-title":"Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1429.e1","DOI":"10.1016\/j.cmi.2019.03.023","article-title":"Impact of EUCAST ceftaroline breakpoint change on the susceptibility of methicillin-resistant Staphylococcus aureus isolates collected from patients with complicated skin and soft-tissue infections","volume":"25","author":"Stone","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wintachai, P., Surachat, K., and Singkhamanan, K. (2022). Isolation and Characterization of a Novel Autographiviridae Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant Acinetobacter baumannii-Associated Skin and Soft Tissue Infections. Viruses, 14.","DOI":"10.3390\/v14020194"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/gigascience\/gix120","article-title":"SOAPnuke: A MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughput sequencing data","volume":"7","author":"Chen","year":"2018","journal-title":"GigaScience"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/bioinformatics\/btp698","article-title":"Fast and accurate long-read alignment with Burrows-Wheeler transform","volume":"26","author":"Li","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1093\/nar\/gkq275","article-title":"Ab initio gene identification in metagenomic sequences","volume":"38","author":"Zhu","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/nar\/gkq901","article-title":"ViralZone: A knowledge resource to understand virus diversity","volume":"39","author":"Hulo","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","article-title":"Mauve: Multiple alignment of conserved genomic sequence with rearrangements","volume":"14","author":"Darling","year":"2004","journal-title":"Genome Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: A genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1038\/s41396-019-0397-9","article-title":"Active and diverse viruses persist in the deep sub-seafloor sediments over thousands of years","volume":"13","author":"Cai","year":"2019","journal-title":"ISME J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1093\/nar\/gkx932","article-title":"Virus taxonomy: The database of the International Committee on Taxonomy of Viruses (ICTV)","volume":"46","author":"Lefkowitz","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"McCutcheon, J.G., Lin, A., and Dennis, J.J. (2020). Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176338"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s11262-020-01818-5","article-title":"Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548","volume":"57","author":"Zhang","year":"2021","journal-title":"Virus. Genes."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"000015","DOI":"10.1099\/acmi.0.000015","article-title":"Phylogenomics insights into order and families of Lysobacterales","volume":"1","author":"Kumar","year":"2019","journal-title":"Access Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5190","DOI":"10.7717\/peerj.5190","article-title":"Experimental evolution of UV resistance in a phage","volume":"6","author":"Tom","year":"2018","journal-title":"PeerJ"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1007\/s00284-020-02077-1","article-title":"Characterization and Genome Analysis of a Novel Marine Alteromonas Phage P24","volume":"77","author":"Zhang","year":"2020","journal-title":"Curr. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/s41396-019-0478-9","article-title":"Viruses as key reservoirs of antibiotic resistance genes in the environment","volume":"13","author":"Debroas","year":"2019","journal-title":"ISME J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"46004","DOI":"10.1074\/jbc.M108627200","article-title":"Structure and Function of the Escherichia coli RecE Protein, a Member of the RecB Nuclease Domain Family*","volume":"276","author":"Chang","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/nature02988","article-title":"Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks","volume":"432","author":"Singleton","year":"2004","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3285","DOI":"10.1016\/j.jmb.2015.08.013","article-title":"Architecture of the Complex Formed by Large and Small Terminase Subunits from Bacteriophage P22","volume":"427","author":"McNulty","year":"2015","journal-title":"J. Mol. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4618","DOI":"10.1074\/jbc.M208574200","article-title":"Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity","volume":"278","author":"Baumann","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.coviro.2018.09.004","article-title":"Phage assembly and the special role of the portal protein","volume":"31","author":"Prevelige","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.mib.2008.09.004","article-title":"Bacteriophage genomics","volume":"11","author":"Hatfull","year":"2008","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"30691","DOI":"10.1074\/jbc.M111.247999","article-title":"Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans","volume":"286","author":"McSweeney","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.3389\/fmicb.2018.01033","article-title":"Thousands of Novel Endolysins Discovered in Uncultured Phage Genomes","volume":"9","author":"Coutinho","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.1021\/acsinfecdis.1c00222","article-title":"Membrane-Permeable Antibacterial Enzyme against Multidrug-Resistant Acinetobacter baumannii","volume":"7","author":"Chen","year":"2021","journal-title":"ACS Infect. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1709\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:01:29Z","timestamp":1760140889000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,2]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["v14081709"],"URL":"https:\/\/doi.org\/10.3390\/v14081709","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,2]]}}}