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With the continuous emergence of PRV variants and the increasing number of cases of human infection, there is an urgent need to develop antiviral drugs. In this study, we discover that Glycyrrhiza polysaccharide (GCP) has anti-PRV infection activity in vitro, and 600 \u03bcg\/mL GCP can completely block viral infection. The addition of GCP simultaneously with or after PRV infection had a significant inhibitory effect on PRV. Addition of GCP at different times of the virus life cycle mainly led to the inhibition of the attachment and internalization of PRV but does not affect viral replication and release. Our findings suggest that GCP has potential as a drug against PRV infection.<\/jats:p>","DOI":"10.3390\/v14081772","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T03:40:32Z","timestamp":1660621232000},"page":"1772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Glycyrrhiza Polysaccharide Inhibits Pseudorabies Virus Infection by Interfering with Virus Attachment and Internalization"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-8893","authenticated-orcid":false,"given":"Changchao","family":"Huan","sequence":"first","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Xu","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ni","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Gao","sequence":"additional","affiliation":[{"name":"Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"},{"name":"China Animal Health and Epidemiology Center, Qingdao 266011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0147-9571(91)90128-Z","article-title":"Molecular biology of pseudorabies (Aujeszky\u2019s disease) virus","volume":"14","author":"Mettenleiter","year":"1991","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_2","first-page":"65","article-title":"A review of pseudorabies (Aujeszky\u2019s disease) in pigs","volume":"20","author":"Lee","year":"1979","journal-title":"Can. Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1007\/s12250-020-00340-0","article-title":"Current Status and Challenge of Pseudorabies Virus Infection in China","volume":"36","author":"Tan","year":"2021","journal-title":"Virol. Sin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1080\/01652176.1995.9694556","article-title":"Pseudorabies virus eradication by area-wide vaccination is feasible","volume":"17","author":"Stegeman","year":"1995","journal-title":"Vet. Q."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetmic.2016.11.019","article-title":"Vaccines against pseudorabies virus (PrV)","volume":"206","author":"Freuling","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Delva, J.L., Nauwynck, H.J., Mettenleiter, T.C., and Favoreel, H.W. (2020). The Attenuated Pseudorabies Virus Vaccine Strain Bartha K61: A Brief Review on the Knowledge Gathered During 60 Years of Research. Pathogens, 9.","DOI":"10.3390\/pathogens9110897"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.vetmic.2015.09.021","article-title":"Emergence of a Pseudorabies virus variant with increased virulence to piglets","volume":"181","author":"Tong","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Li, A., Lu, G., Qi, J., Wu, L., Tian, K., Luo, T., Shi, Y., Yan, J., and Gao, G.F. (2017). Structural basis of nectin-1 recognition by pseudorabies virus glycoprotein D. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006314"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular biology of pseudorabies virus: Impact on neurovirology and veterinary medicine","volume":"69","author":"Pomeranz","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10179","DOI":"10.1128\/JVI.77.19.10179-10185.2003","article-title":"Herpesvirus entry: An update","volume":"77","author":"Spear","year":"2003","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1055\/s-0035-1557893","article-title":"The Pharmacological Activities of Licorice","volume":"81","author":"Yang","year":"2015","journal-title":"Planta Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"113216","DOI":"10.1016\/j.jep.2020.113216","article-title":"The anti-diabetic activity of licorice, a widely used Chinese herb","volume":"263","author":"Yang","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, C., Zhao, P., Shao, Q., Chen, W., Huang, S., Wang, X., Zhang, C., and He, L. (2022). Effects of dietary Glycyrrhiza polysaccharide on growth performance, blood parameters and immunity in weaned piglets. J. Anim. Physiol. Anim. Nutr., 13692.","DOI":"10.1111\/jpn.13692"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.ijbiomac.2019.11.245","article-title":"Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra","volume":"144","author":"Mutaillifu","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ayeka, P.A., Bian, Y., Githaiga, P.M., and Zhao, Y. (2017). The immunomodulatory activities of licorice polysaccharides (Glycyrrhiza uralensis Fisch.) in CT 26 tumor-bearing mice. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-2030-7"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1039\/D0FO00608D","article-title":"Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide","volume":"11","author":"Wang","year":"2020","journal-title":"Food Funct."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.jep.2009.07.009","article-title":"Aqueous extracts and polysaccharides from liquorice roots (Glycyrrhiza glabra L.) inhibit adhesion of Helicobacter pylori to human gastric mucosa","volume":"125","author":"Wittschier","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.intimp.2007.10.006","article-title":"Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis Fish","volume":"8","author":"Cheng","year":"2008","journal-title":"Int. Immunopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Aipire, A., Yuan, P., Aimaier, A., Cai, S., Mahabati, M., Lu, J., Ying, T., Zhang, B., and Li, J. (2020). Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis. Biomolecules, 10.","DOI":"10.3390\/biom10010159"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ijbiomac.2017.04.006","article-title":"Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities","volume":"102","author":"Wu","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.vaccine.2017.01.003","article-title":"Bartha-k61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus","volume":"35","author":"Zhou","year":"2017","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1080\/01652176.1985.9693982","article-title":"Vaccines against Aujeszky\u2019s disease: Evaluation of their efficacy under standardized laboratory conditions","volume":"7","year":"1985","journal-title":"Vet. Q."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.vetmic.2014.09.003","article-title":"Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China","volume":"174","author":"Luo","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"369","DOI":"10.4142\/jvs.2016.17.3.369","article-title":"Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013\u20132014","volume":"17","author":"Fan","year":"2016","journal-title":"J. Vet. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.1111\/tbed.13297","article-title":"Dynamic cerebrospinal fluid analyses of severe pseudorabies encephalitis","volume":"66","author":"Zheng","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.3201\/eid2406.171612","article-title":"Human Endophthalmitis Caused By Pseudorabies Virus Infection, China, 2017","volume":"24","author":"Ai","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e3690","DOI":"10.1093\/cid\/ciaa987","article-title":"A Novel Human Acute Encephalitis Caused by Pseudorabies Virus Variant Strain","volume":"73","author":"Liu","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"198556","DOI":"10.1016\/j.virusres.2021.198556","article-title":"Quercetin as an antiviral agent inhibits the Pseudorabies virus in vitro and in vivo","volume":"305","author":"Sun","year":"2021","journal-title":"Virus Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"795593","DOI":"10.3389\/fmicb.2021.795593","article-title":"Meclizine Inhibits Pseudorabies Virus Replication by Interfering With Virus Entry and Release","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"He, W., Zhai, X., Su, J., Ye, R., Zheng, Y., and Su, S. (2019). Antiviral Activity of Germacrone against Pseudorabies Virus in Vitro. Pathogens, 8.","DOI":"10.3390\/pathogens8040258"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109313","DOI":"10.1016\/j.vetmic.2021.109313","article-title":"A natural product, (S)-10-Hydroxycamptothecin inhibits pseudorabies virus proliferation through DNA damage dependent antiviral innate immunity","volume":"265","author":"Liu","year":"2022","journal-title":"Vet. Microbiol"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhao, X., Tong, W., Song, X., Jia, R., Li, L., Zou, Y., He, C., Liang, X., Lv, C., and Jing, B. (2018). Antiviral Effect of Resveratrol in Piglets Infected with Virulent Pseudorabies Virus. Viruses, 10.","DOI":"10.3390\/v10090457"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.antiviral.2009.10.014","article-title":"Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus","volume":"85","author":"Sui","year":"2010","journal-title":"Antiviral Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ijbiomac.2018.04.056","article-title":"The antiviral activity of polysaccharides and their derivatives","volume":"115","author":"Chen","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Wang, X., Luo, J., Wen, Z., Shuai, L., Wang, C., Zhong, G., He, X., Cao, H., Liu, R., and Ge, J. (2022). Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1010343"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1099\/vir.0.054270-0","article-title":"Antiviral activity of sulfated Chuanmingshen violaceum polysaccharide against Newcastle disease virus","volume":"94","author":"Song","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.ijbiomac.2018.12.010","article-title":"Analysis of interaction between sulfated polysaccharides and HIV oligopeptides by surface plasmon resonance","volume":"125","author":"Battulga","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.bbrc.2008.08.100","article-title":"Structure and anti-dengue virus activity of sulfated polysaccharide from a marine alga","volume":"376","author":"Hidari","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.carbpol.2018.06.096","article-title":"The inhibitory effects and mechanisms of 3,6-O-sulfated chitosan against human papillomavirus infection","volume":"198","author":"Gao","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1093\/glycob\/cwi031","article-title":"Multiple and multivalent interactions of novel anti-AIDS drug candidates, sulfated polymannuronate (SPMG)-derived oligosaccharides, with gp120 and their anti-HIV activities","volume":"15","author":"Liu","year":"2005","journal-title":"Glycobiology"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1772\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:08:33Z","timestamp":1760141313000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/8\/1772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,14]]},"references-count":41,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["v14081772"],"URL":"https:\/\/doi.org\/10.3390\/v14081772","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,14]]}}}