{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:28:39Z","timestamp":1792704519906,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T00:00:00Z","timestamp":1661644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"NIH\/NIAID","doi-asserted-by":"publisher","award":["P01AI120943"],"award-info":[{"award-number":["P01AI120943"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"NIH\/NIAID","doi-asserted-by":"publisher","award":["UC7AI095321"],"award-info":[{"award-number":["UC7AI095321"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors involved in actin nucleation, such as Rac1 and Arp2\/3, were shown important in both steps. However, downstream of nucleation, many other cell factors are needed to control actin dynamics. How these regulate EBOV infection remains largely unclear. Here, we identified the actin-regulating protein, CAPG, as important for EBOV replication. Notably, knockdown of CAPG specifically inhibited viral infectivity and yield of infectious particles. Cell-based mechanistic analysis revealed a requirement of CAPG for virus production from infected cells. Proximity ligation and split-green fluorescent protein reconstitution assays revealed strong association of CAPG with VP40 that was mediated through the S1 domain of CAPG. Overall, CAPG is a novel host factor regulating EBOV infection through connecting actin filament stabilization to viral egress from cells.<\/jats:p>","DOI":"10.3390\/v14091903","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T02:09:36Z","timestamp":1661911776000},"page":"1903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2390-4016","authenticated-orcid":false,"given":"Hiroyuki","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Microbiology, NEIDL, Boston University School of Medicine, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Connell","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Callie J.","family":"Donahue","sequence":"additional","affiliation":[{"name":"Department of Microbiology, NEIDL, Boston University School of Medicine, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8524-8376","authenticated-orcid":false,"given":"RuthMabel","family":"Boytz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, NEIDL, Boston University School of Medicine, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen Thi Kim","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy W.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 98195, USA"},{"name":"Department of Medicine, Washington University School of Medicine, St. Louis, MO 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3819-9321","authenticated-orcid":false,"given":"Douglas J.","family":"LaCount","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9168-2892","authenticated-orcid":false,"given":"Robert A.","family":"Davey","sequence":"additional","affiliation":[{"name":"Department of Microbiology, NEIDL, Boston University School of Medicine, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1146\/annurev-pathol-052016-100506","article-title":"The Pathogenesis of Ebola Virus Disease","volume":"12","author":"Baseler","year":"2017","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1056\/NEJMra1901594","article-title":"Ebola","volume":"382","author":"Feldmann","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0168-1702(95)00080-1","article-title":"Differentiation of Filoviruses by Electron Microscopy","volume":"39","author":"Geisbert","year":"1995","journal-title":"Virus Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0042-6822(85)90236-3","article-title":"Descriptive Analysis of Ebola Virus Proteins","volume":"147","author":"Elliott","year":"1985","journal-title":"Virology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1099\/vir.0.019794-0","article-title":"Characterization of the Ebola Virus Nucleoprotein-RNA Complex","volume":"91","author":"Noda","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13871","DOI":"10.1073\/pnas.250277297","article-title":"A PPxY Motif within the VP40 Protein of Ebola Virus Interacts Physically and Functionally with a Ubiquitin Ligase: Implications for Filovirus Budding","volume":"97","author":"Harty","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1128\/JVI.72.4.3155-3160.1998","article-title":"Characterization of Ebola Virus Entry by Using Pseudotyped Viruses: Identification of Receptor-Deficient Cell Lines","volume":"72","author":"Bates","year":"1998","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Nanbo, A., Imai, M., Watanabe, S., Noda, T., Takahashi, K., Neumann, G., Halfmann, P., and Kawaoka, Y. (2010). Ebolavirus Is Internalized into Host Cells via Macropinocytosis in a Viral Glycoprotein-Dependent Manner. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1001121"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Saeed, M.F., Kolokoltsov, A.A., Albrecht, T., and Davey, R.A. (2010). Cellular Entry of Ebola Virus Involves Uptake by a Macropinocytosis-Like Mechanism and Subsequent Trafficking through Early and Late Endosomes. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1001110"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"R619","DOI":"10.1016\/j.cub.2021.01.036","article-title":"The Cytoskeleton in Phagocytosis and Macropinocytosis","volume":"31","author":"Mylvaganam","year":"2021","journal-title":"Curr. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Saeed, M.F., Kolokoltsov, A.A., Freiberg, A.N., Holbrook, M.R., and Davey, R.A. (2008). Phosphoinositide-3 Kinase-Akt Pathway Controls Cellular Entry of Ebola Virus. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000141"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10176","DOI":"10.1128\/JVI.00422-09","article-title":"Rho GTPases Modulate Entry of Ebola Virus and Vesicular Stomatitis Virus Pseudotyped Vectors","volume":"83","author":"Quinn","year":"2009","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S160","DOI":"10.1093\/infdis\/jiv083","article-title":"Transport of Ebolavirus Nucleocapsids Is Dependent on Actin Polymerization: Live-Cell Imaging Analysis of Ebolavirus-Infected Cells","volume":"212","author":"Schudt","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S1097-2765(02)00588-9","article-title":"The Assembly of Ebola Virus Nucleocapsid Requires Virion-Associated Proteins 35 and 24 and Posttranslational Modification of Nucleoprotein","volume":"10","author":"Huang","year":"2002","journal-title":"Mol. Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"S892","DOI":"10.1093\/infdis\/jir311","article-title":"Knockdown of Ebola Virus VP24 Impairs Viral Nucleocapsid Assembly and Prevents Virus Replication","volume":"204","author":"Mateo","year":"2011","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S284","DOI":"10.1086\/520582","article-title":"Ebola Virus (EBOV) VP24 Inhibits Transcription and Replication of the EBOV Genome","volume":"196","author":"Watanabe","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Grikscheit, K., Dolnik, O., Takamatsu, Y., Pereira, A.R., and Becker, S. (2020). Ebola Virus Nucleocapsid-Like Structures Utilize Arp2\/3 Signaling for Intracellular Long-Distance Transport. Cells, 9.","DOI":"10.3390\/cells9071728"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4855","DOI":"10.1128\/JVI.76.10.4855-4865.2002","article-title":"Ebola Virus VP40 Drives the Formation of Virus-Like Filamentous Particles Along with GP","volume":"76","author":"Noda","year":"2002","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1128\/JVI.77.3.1812-1819.2003","article-title":"Overlapping Motifs (PTAP and PPEY) within the Ebola Virus VP40 Protein Function Independently as Late Budding Domains: Involvement of Host Proteins TSG101 and VPS-4","volume":"77","author":"Licata","year":"2003","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"L41","DOI":"10.1016\/j.bpj.2012.09.026","article-title":"Single-Particle Tracking Demonstrates That Actin Coordinates the Movement of the Ebola Virus Matrix Protein","volume":"103","author":"Digman","year":"2012","journal-title":"Biophys. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/1743-422X-2-92","article-title":"Packaging of Actin into Ebola Virus VLPs","volume":"2","author":"Han","year":"2005","journal-title":"Virol. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.bbrc.2018.07.159","article-title":"Direct Effects of Ca2+\/Calmodulin on Actin Filament Formation","volume":"506","author":"Izadi","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0955-0674(99)00062-9","article-title":"Control of Actin Assembly and Disassembly at Filament Ends","volume":"12","author":"Cooper","year":"2000","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1126\/science.2255912","article-title":"GCap39, a Calcium Ion- and Polyphosphoinositide-Regulated Actin Capping Protein","volume":"250","author":"Yu","year":"1990","journal-title":"Science"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6418","DOI":"10.1038\/sj.onc.1204783","article-title":"Cortactin: Coupling Membrane Dynamics to Cortical Actin Assembly","volume":"20","author":"Weed","year":"2001","journal-title":"Oncogene"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.antiviral.2017.11.016","article-title":"Retro-2 and Its Dihydroquinazolinone Derivatives Inhibit Filovirus Infection","volume":"149","author":"Shtanko","year":"2018","journal-title":"Antiviral Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"12070","DOI":"10.1128\/JVI.01743-06","article-title":"Mutation of YMYL in the Nipah Virus Matrix Protein Abrogates Budding and Alters Subcellular Localization","volume":"80","author":"Ciancanelli","year":"2006","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1128\/JVI.73.3.2333-2342.1999","article-title":"Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems","volume":"73","author":"Weik","year":"1999","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1038\/srep02854","article-title":"A New Protein-Protein Interaction Sensor Based on Tripartite Split-GFP Association","volume":"3","author":"Cabantous","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4526","DOI":"10.1021\/jacs.8b13042","article-title":"Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals","volume":"141","author":"Zhang","year":"2019","journal-title":"J. Am. Chem. Soc."},{"key":"ref_31","unstructured":"Mori, H., and Davey, R.A. (2022). CellProfiler Pipelines Used for CAPG-Ebola Analysis. Zenodo."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1128\/AAC.00543-16","article-title":"Large-Scale Screening and Identification of Novel Ebola Virus and Marburg Virus Entry Inhibitors","volume":"60","author":"Anantpadma","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1016\/j.cell.2018.08.044","article-title":"Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication","volume":"175","author":"Batra","year":"2018","journal-title":"Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s12250-012-3252-y","article-title":"Rapid Detection of Filoviruses by Real-Time TaqMan Polymerase Chain Reaction Assays","volume":"27","author":"Huang","year":"2012","journal-title":"Virol. Sin."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4125","DOI":"10.1128\/JCM.39.11.4125-4130.2001","article-title":"Development and Evaluation of a Fluorogenic 5\u2032 Nuclease Assay To Detect and Differentiate between Ebola Virus Subtypes Zaire and Sudan","volume":"39","author":"Gibb","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/1348-0421.12475","article-title":"Rapid Detection and Quantification of Ebola Zaire Virus by One-Step Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction: Ebola Virus Detection by QRT-PCR Assay","volume":"61","author":"Ro","year":"2017","journal-title":"Microbiol. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1038\/srep01206","article-title":"The Spatio-Temporal Distribution Dynamics of Ebola Virus Proteins and RNA in Infected Cells","volume":"3","author":"Nanbo","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1006\/geno.1994.1543","article-title":"The Human Actin-Regulatory Protein Cap G: Gene Structure and Chromosome Location","volume":"23","author":"Mishra","year":"1994","journal-title":"Genomics"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Salanga, C.M., and Salanga, M.C. (2021). Genotype to Phenotype: CRISPR Gene Editing Reveals Genetic Compensation as a Mechanism for Phenotypic Disjunction of Morphants and Mutants. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073472"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/nmeth947","article-title":"Direct Observation of Individual Endogenous Protein Complexes in Situ by Proximity Ligation","volume":"3","author":"Gullberg","year":"2006","journal-title":"Nat. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kirchdoerfer, R.N., Moyer, C.L., Abelson, D.M., and Saphire, E.O. (2016). The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis. PLOS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005937"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"R116","DOI":"10.1186\/bcr3585","article-title":"A Nanobody Targeting the F-Actin Capping Protein CapG Restrains Breast Cancer Metastasis","volume":"15","author":"Bethuyne","year":"2013","journal-title":"Breast Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kesari, A.S., Aryal, U.K., and LaCount, D.J. (2020). A Novel Proximity Biotinylation Assay Based on the Self-Associating Split GFP1-10\/11. Proteomes, 8.","DOI":"10.3390\/proteomes8040037"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1038\/nm1201-1313","article-title":"HIV-1 and Ebola Virus Encode Small Peptide Motifs That Recruit Tsg101 to Sites of Particle Assembly to Facilitate Egress","volume":"7","author":"Zang","year":"2001","journal-title":"Nat. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/cm.20226","article-title":"Versatile Fluorescent Probes for Actin Filaments Based on the Actin-Binding Domain of Utrophin","volume":"64","author":"Burkel","year":"2007","journal-title":"Cell Motil. Cytoskeleton"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4458","DOI":"10.1038\/sj.emboj.7601323","article-title":"A CapG Gain-of-Function Mutant Reveals Critical Structural and Functional Determinants for Actin Filament Severing","volume":"25","author":"Zhang","year":"2006","journal-title":"EMBO J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e104006","DOI":"10.15252\/embj.2019104006","article-title":"Structural Insights into Actin Filament Recognition by Commonly Used Cellular Actin Markers","volume":"39","author":"Kumari","year":"2020","journal-title":"EMBO J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1073\/pnas.1211164110","article-title":"Pathway of Actin Filament Branch Formation by Arp2\/3 Complex Revealed by Single-Molecule Imaging","volume":"110","author":"Smith","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"107742","DOI":"10.1016\/j.jsb.2021.107742","article-title":"Dual-Axis Volta Phase Plate Cryo-Electron Tomography of Ebola Virus-like Particles Reveals Actin-VP40 Interactions","volume":"213","author":"Winter","year":"2021","journal-title":"J. Struct. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1083\/jcb.200101113","article-title":"Comparisons of CapG and Gelsolin-Null Macrophages","volume":"154","author":"Witke","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.1128\/JVI.02695-12","article-title":"Ebola Virus Exploits a Monocyte Differentiation Program To Promote Its Entry","volume":"87","author":"Martinez","year":"2013","journal-title":"J. Virol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.yjmcc.2021.06.001","article-title":"Exogenous Extracellular Matrix Proteins Decrease Cardiac Fibroblast Activation in Stiffening Microenvironment through CAPG","volume":"159","author":"Wang","year":"2021","journal-title":"J. Mol. Cell. Cardiol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1903\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:19:05Z","timestamp":1760141945000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,28]]},"references-count":53,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["v14091903"],"URL":"https:\/\/doi.org\/10.3390\/v14091903","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.04.10.487814","asserted-by":"object"}]},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,28]]}}}