{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T05:57:01Z","timestamp":1780725421890,"version":"3.54.1"},"reference-count":56,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Members of the jingmenviruses group have been found in arthropods and mammals on all continents except Australia and Antarctica. Two viruses of this group were isolated from patients with fever after a tick bite. Using a nested RT-PCR assay targeting a jingmenvirus polymerase gene fragment, we screened ticks collected in seven regions of Russia and found that the abundant jingmenvirus-positive were of Ixodes ricinus species, with the prevalence ranging from 19.8% to 34.3%. In all cases, DNase\/RNase treatment suggested that the detected molecule was DNA and subsequent next generation sequencing (NGS) proved that the viral polymerase gene was integrated in the I. ricinus genome. The copy number of the integrated polymerase gene was quantified by qPCR relative to the ITS2 gene and estimated as 1.32 copies per cell. At least three different genetic variants of the integrated polymerase gene were found in the territory of Russia. Phylogenetic analysis of the integrated jingmenvirus polymerase gene showed the highest similarity with the sequence of the correspondent gene obtained in Serbia from I. ricinus.<\/jats:p>","DOI":"10.3390\/v14091908","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T02:09:36Z","timestamp":1661911776000},"page":"1908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Integrated Jingmenvirus Polymerase Gene in Ixodes ricinus Genome"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8407-2623","authenticated-orcid":false,"given":"Evgeny S.","family":"Morozkin","sequence":"first","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6835-4572","authenticated-orcid":false,"given":"Marat T.","family":"Makenov","sequence":"additional","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga B.","family":"Zhurenkova","sequence":"additional","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-7081","authenticated-orcid":false,"given":"Ivan S.","family":"Kholodilov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-0774","authenticated-orcid":false,"given":"Oxana A.","family":"Belova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina V.","family":"Radyuk","sequence":"additional","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina 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Medicine, 117246 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Korneenko","sequence":"additional","affiliation":[{"name":"Multiomics Research Laboratory, Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana A.","family":"Voizekhovskaya","sequence":"additional","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey D.","family":"Neverov","sequence":"additional","affiliation":[{"name":"Group of Molecular Diagnostics and Epidemiology of Zoonotic Diseases, Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, 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Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1056\/NEJMoa1805068","article-title":"A new segmented virus associated with human febrile illness in China","volume":"380","author":"Wang","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kholodilov, I.S., Belova, O.A., Morozkin, E.S., Litov, A.G., Ivannikova, A.Y., Makenov, M.T., Shchetinin, A.M., Aibulatov, S.V., Bazarova, G.K., and Bell-Sakyi, L. (2021). Geographical and tick-dependent distribution of Flavi-like Alongshan and Yanggou tick viruses in Russia. Viruses, 13.","DOI":"10.3390\/v13030458"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kobayashi, D., Kuwata, R., Kimura, T., Shimoda, H., Fujita, R., Faizah, A.N., Kai, I., Matsumura, R., Kuroda, Y., and Watanabe, S. (2021). Detection of Jingmenviruses in Japan with evidence of vertical transmission in ticks. Viruses, 13.","DOI":"10.3390\/v13122547"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ebiom.2019.04.004","article-title":"Emergence of human infection with Jingmen tick virus in China: A retrospective study","volume":"43","author":"Jia","year":"2019","journal-title":"EBioMedicine"},{"key":"ref_6","first-page":"129","article-title":"Detection of the RNA for new multicomponent virus in patients with Crimean-Congo hemorrhagic fever in Southern Russia","volume":"75","author":"Ternovoi","year":"2020","journal-title":"Vestn. Ross. Akad. Meditsinskikh Nauk"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.meegid.2018.07.010","article-title":"Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever Orthonairovirus in humans, Kosovo","volume":"65","author":"Emmerich","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kholodilov, I.S., Litov, A.G., Klimentov, A.S., Belova, O.A., Polienko, A.E., Nikitin, N.A., Shchetinin, A.M., Ivannikova, A.Y., Bell-Sakyi, L., and Yakovlev, A.S. (2020). Isolation and Characterisation of Alongshan Virus in Russia. Viruses, 12.","DOI":"10.3390\/v12040362"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/1560-7917.ES.2019.24.27.1900394","article-title":"Detection of novel tick-borne pathogen, Alongshan virus, in Ixodes ricinus ticks, South-Eastern Finland, 2019","volume":"24","author":"Kuivanen","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Din\u00e7er, E., Hac\u0131oglu, S., Kar, S., Emanet, N., Brinkmann, A., Nitsche, A., \u00d6zkul, A., Linton, Y.-M., and Erg\u00fcnay, K. (2019). Survey and characterization of Jingmen tick virus variants. Viruses, 11.","DOI":"10.3390\/v11111071"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/ve\/veaa051","article-title":"Diversity and circulation of Jingmen tick virus in ticks and mammals","volume":"6","author":"Guo","year":"2020","journal-title":"Virus Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e00645-19","DOI":"10.1128\/mSphere.00645-19","article-title":"Insights into the host range, genetic diversity, and geographical distribution of Jingmenviruses","volume":"4","author":"Temmam","year":"2019","journal-title":"mSphere"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ternovoi, V.A., Protopopova, E.V., Shvalov, A.N., Kartashov, M.Y., Bayandin, R.B., Tregubchak, T.V., Yakovlev, S.A., Nikiforov, K.A., Konovalova Svetlana, N., and Loktev, V.B. (2020). Complete coding genome sequence for a novel multicomponent kindia tick virus detected from ticks collected in Guinea. bioRxiv, 4\u20139.","DOI":"10.1101\/2020.04.11.036723"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.3201\/eid2608.190986","article-title":"Presence of segmented Flavivirus Infections in North America","volume":"26","author":"Vandegrift","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1128\/genomeA.00232-17","article-title":"Complete coding genome sequence for Mogiana Tick Virus, a Jingmenvirus isolated from ticks in Brazil","volume":"5","author":"Villa","year":"2017","journal-title":"Genome Announc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10441","DOI":"10.1016\/j.meegid.2020.104411","article-title":"Identification and characterization of Jingmen tick virus in rodents from Xinjiang, China","volume":"84","author":"Yu","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1590\/0074-0276130166","article-title":"Characterisation of divergent Flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil","volume":"109","author":"Maruyama","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gondard, M., Temmam, S., Devillers, E., Pinarello, V., Bigot, T., Chr\u00e9tien, D., Aprelon, R., Vayssier-Taussat, M., Albina, E., and Eloit, M. (2020). RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and cattle susceptibility in the French Antilles. Viruses, 12.","DOI":"10.3390\/v12020144"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.chom.2011.09.002","article-title":"The evolution of endogenous viral elements","volume":"10","author":"Holmes","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Katzourakis, A., and Gifford, R.J. (2010). Endogenous viral elements in animal genomes. PLoS Genet., 6.","DOI":"10.1371\/journal.pgen.1001191"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1099\/vir.0.79850-0","article-title":"Sequences of Flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. Mosquitoes","volume":"85","author":"Crochu","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-6-93","article-title":"Detection of novel insect Flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italy","volume":"6","author":"Roiz","year":"2009","journal-title":"Virol. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.00571-17","article-title":"Uncovering the repertoire of endogenous Flaviviral elements in Aedes mosquito genomes","volume":"91","author":"Suzuki","year":"2017","journal-title":"J. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1111\/mec.15798","article-title":"Population genomics in the arboviral vector Aedes aegypti reveals the genomic architecture and evolution of endogenous viral elements","volume":"30","author":"Crava","year":"2021","journal-title":"Mol. Ecol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-021-04828-w","article-title":"Flavivirus integrations in Aedes aegypti are limited and highly conserved across samples from different geographic regions unlike integrations in Aedes albopictus","volume":"14","author":"Spadar","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/ve\/vew035","article-title":"Discovery of Flavivirus-derived endogenous viral elements in Anopheles mosquito genomes supports the existence of Anopheles-associated insect-specific Flaviviruses","volume":"3","author":"Lequime","year":"2017","journal-title":"Virus Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/ve\/vez010","article-title":"Novel insights into endogenous RNA viral elements in Ixodes scapularis and other arbovirus vector genomes","volume":"5","author":"Russo","year":"2019","journal-title":"Virus Evol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e02124-18","DOI":"10.1128\/JVI.02124-18","article-title":"Endogenous viral elements are widespread in arthropod genomes and commonly give rise to PIWI-interacting RNAs","volume":"93","author":"Nigg","year":"2019","journal-title":"J. Virol."},{"key":"ref_29","unstructured":"Filippova, N.A. (1977). Ixodid Ticks of Subfamily Ixodinae \/\/ Fauna SSSR Paukoobraznye, Nauka."},{"key":"ref_30","unstructured":"Filippova, N.A. (1997). Ixodid Ticks of Subfamily Ambyomminae \/\/ Fauna of Russia and Neighbouring Countries. Arachnoidea, Nauka."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101265","DOI":"10.1016\/j.ttbdis.2019.101265","article-title":"First detection of tick-borne encephalitis virus in Ixodes ricinus ticks and their rodent hosts in Moscow, Russia","volume":"10","author":"Makenov","year":"2019","journal-title":"Ticks Tick. Borne Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ttbdis.2015.09.005","article-title":"Natural hybridization between Ixodes ricinus and Ixodes persulcatus ticks evidenced by molecular genetics methods","volume":"7","author":"Kovalev","year":"2016","journal-title":"Ticks Tick. Borne Dis."},{"key":"ref_33","unstructured":"Scherer, R. (2022, August 26). PropCIs: Various Confidence Interval Methods for Proportions. Available online: https:\/\/cran.r-project.org\/package=PropCIs."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0167-5877(98)00131-7","article-title":"Comparison of methods for estimation of individual-level prevalence based on pooled samples","volume":"39","author":"Cowling","year":"1999","journal-title":"Prev. Vet. Med."},{"key":"ref_35","unstructured":"Sergeant, E. (2022, August 26). Epitools Epidemiological Calculators. Available online: http:\/\/epitools.ausvet.com.au."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1577\/1548-8667(2001)013<0300:ACOMOS>2.0.CO;2","article-title":"A Critique of methods of sampling and reporting pathogens in populations of fish","volume":"13","author":"Williams","year":"2001","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1093\/oxfordjournals.molbev.a026334","article-title":"Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis","volume":"17","author":"Castresana","year":"2000","journal-title":"Mol. Biol. Evol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_39","unstructured":"Tavar\u00e9, S. (1986). Some probabilistic and statistical problems in the analysis of dna sequences. Lectures on Mathematics in the Life Sciences, American Mathematical Society."},{"key":"ref_40","first-page":"678","article-title":"Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases","volume":"9","author":"Tamura","year":"1992","journal-title":"Mol. Biol. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Stanojevi\u0107, M., Li, K., Stamenkovi\u0107, G., Ili\u0107, B., Paunovi\u0107, M., Pe\u0161i\u0107, B., Maslovara, I.\u00d0., \u0160ilji\u0107, M., \u0106irkovi\u0107, V., and Zhang, Y. (2020). Depicting the RNA virome of hematophagous arthropods from Belgrade, Serbia. Viruses, 12.","DOI":"10.3390\/v12090975"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Estrada-Pe\u00f1a, A., Pf\u00e4ffle, M.P., and Petney, T.N. (2017). Genus Ixodes Latreille, 1795. Ticks of Europe and North Africa. A Guide to Species Identification, Springer.","DOI":"10.1007\/978-3-319-63760-0_18"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.pt.2007.07.009","article-title":"Tick cell lines: Tools for tick and tick-borne disease research","volume":"23","author":"Zweygarth","year":"2007","journal-title":"Trends Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12923","DOI":"10.1038\/s41598-019-49641-9","article-title":"A Transcriptome-based phylogenetic study of hard ticks (Ixodidae)","volume":"9","author":"Charrier","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.chom.2016.07.011","article-title":"A Multicomponent animal virus isolated from mosquitoes","volume":"20","author":"Ladner","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.virol.2015.02.011","article-title":"Endogenous Viruses: Connecting recent and ancient viral evolution","volume":"479\u2013480","author":"Aiewsakun","year":"2015","journal-title":"Virology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms12410","article-title":"Virus-derived DNA drives mosquito vector tolerance to arboviral infection","volume":"7","author":"Goic","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.cois.2021.11.005","article-title":"RNA virus EVEs in insect genomes","volume":"49","author":"Wallau","year":"2022","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.41244","article-title":"Control of RNA viruses in mosquito cells through the acquisition of vDNA and endogenous viral elements","volume":"8","author":"Tassetto","year":"2019","journal-title":"eLife"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12862-018-1324-9","article-title":"Correction to: Diversity and evolution of the transposable element repertoire in arthropods with particular reference to insects","volume":"19","author":"Petersen","year":"2019","journal-title":"BMC Ecol. Evol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev-ento-070720-074650","article-title":"Transposable elements and the evolution of insects","volume":"66","author":"Gilbert","year":"2021","journal-title":"Annu. Rev. Entomol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3511","DOI":"10.1016\/j.cub.2017.09.067","article-title":"The diversity, structure, and function of heritable adaptive immunity sequences in the Aedes aegypti genome","volume":"27","author":"Whitfield","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Palatini, U., Miesen, P., Carballar-Lejarazu, R., Ometto, L., Rizzo, E., Tu, Z., van Rij, R.P., and Bonizzoni, M. (2017). Comparative genomics shows that viral integrations are abundant and express pirnas in the arboviral vectors Aedes aegypti and Aedes albopictus. BMC Genom., 18.","DOI":"10.1186\/s12864-017-3903-3"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1908\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:19:22Z","timestamp":1760141962000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":56,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["v14091908"],"URL":"https:\/\/doi.org\/10.3390\/v14091908","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]}}}