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In this study, a CD2v gene deletion was introduced to a genotype IX virus from East Africa, ASFV-Kenya-IX-1033 (ASFV-Kenya-IX-1033-\u2206CD2v), to investigate whether this deletion led to reduced virulence in domestic pigs and to see if inoculation with this LA-ASFV could induce protective immunity against parental virus challenge. All pigs inoculated with ASFV-Kenya-IX-1033-\u0394CD2v survived inoculation but presented with fever, reduced appetite and lethargy. ASFV genomic copies were detected in only one animal at one time point. Seven out of eight animals survived subsequent challenge with the pathogenic parental strain (87.5%) but had mild to moderate clinical symptoms and had a gross pathology compatible with chronic ASFV infection. All mock-immunised animals developed acute ASF upon challenge with ASFV-Kenya-IX-1033 and were euthanised upon meeting the humane endpoint criteria. ASFV genome copy numbers after challenge were similar in the two groups. ASFV-Kenya-IX-1033-\u2206CD2v is therefore a useful tool to investigate the development of immunity to ASFV genotype IX, but safety concerns preclude its use as a candidate vaccine without further attenuation.<\/jats:p>","DOI":"10.3390\/v14091917","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T02:09:36Z","timestamp":1661911776000},"page":"1917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8764-4156","authenticated-orcid":false,"given":"Johanneke D.","family":"Hemmink","sequence":"first","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-483X","authenticated-orcid":false,"given":"Emmanuel M.","family":"Khazalwa","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5594-4418","authenticated-orcid":false,"given":"Hussein M.","family":"Abkallo","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Oduor","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremiah","family":"Khayumbi","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4539-5806","authenticated-orcid":false,"given":"Nicholas","family":"Svitek","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2002-5462","authenticated-orcid":false,"given":"Sonal P.","family":"Henson","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"},{"name":"Deep Seq, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-5609","authenticated-orcid":false,"given":"Sandra","family":"Blome","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, Insel Riems, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-1916","authenticated-orcid":false,"given":"G\u00fcnther","family":"Keil","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, Insel Riems, 17493 Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard P.","family":"Bishop","sequence":"additional","affiliation":[{"name":"International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3691-3971","authenticated-orcid":false,"given":"Lucilla","family":"Steinaa","sequence":"additional","affiliation":[{"name":"Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s11262-021-01879-0","article-title":"A Systematic Review of Genotypes and Serogroups of African Swine Fever Virus","volume":"58","author":"Qu","year":"2022","journal-title":"Virus Genes"},{"key":"ref_2","unstructured":"(2022, July 01). Animal Health Status of Regions, Available online: https:\/\/www.aphis.usda.gov\/aphis\/ourfocus\/animalhealth\/animal-and-animal-product-import-information\/animal-health-status-of-regions."},{"key":"ref_3","unstructured":"(2022, July 01). Vietnam Officially Announces Successful Production of a Vaccine against African Swine Fever. Available online: https:\/\/Vietnamagriculture.Nongnghiep.vn\/Vietnam-Officially-Announces-Successful-Production-of-a-Vaccine-against-African-Swine-Fever-D324623.Html."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gladue, D.P., and Borca, M.V. (2022). Recombinant ASF Live Attenuated Virus Strains as Experimental Vaccine Candidates. Viruses, 14.","DOI":"10.3390\/v14050878"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10369","DOI":"10.1038\/s41598-017-09948-x","article-title":"Phenotyping and Susceptibility of Established Porcine Cells Lines to African Swine Fever Virus Infection and Viral Production","volume":"7","author":"Riera","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1128\/JVI.03250-14","article-title":"The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome","volume":"89","author":"Krug","year":"2015","journal-title":"J. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1038\/s41598-018-21575-8","article-title":"CRISPR-Cas9, a Tool to Efficiently Increase the Development of Recombinant African Swine Fever Viruses","volume":"8","author":"Borca","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"733674","DOI":"10.3389\/fgene.2021.733674","article-title":"Rapid CRISPR\/Cas9 Editing of Genotype IX African Swine Fever Virus Circulating in Eastern and Central Africa","volume":"12","author":"Abkallo","year":"2021","journal-title":"Front. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4698","DOI":"10.1016\/j.vaccine.2016.08.011","article-title":"Deletion of African Swine Fever Virus Interferon Inhibitors from the Genome of a Virulent Isolate Reduces Virulence in Domestic Pigs and Induces a Protective Response","volume":"34","author":"Reis","year":"2016","journal-title":"Vaccine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/bs.aivir.2017.10.002","article-title":"African Swine Fever Virus Biology and Vaccine Approaches","volume":"100","author":"Revilla","year":"2018","journal-title":"Adv. Virus Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e01340-21","DOI":"10.1128\/JVI.01340-21","article-title":"Role of African Swine Fever Virus Proteins EP153R and EP402R in Reducing Viral Persistence in Blood and Virulence in Pigs Infected with Benin\u0394DP148R","volume":"96","author":"Petrovan","year":"2022","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1128\/JVI.72.4.2881-2889.1998","article-title":"Deletion of a CD2-like Gene, 8-DR, from African Swine Fever Virus Affects Viral Infection in Domestic Swine","volume":"72","author":"Borca","year":"1998","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5312","DOI":"10.1128\/jvi.67.9.5312-5320.1993","article-title":"African Swine Fever Virus Encodes a CD2 Homolog Responsible for the Adhesion of Erythrocytes to Infected Cells","volume":"67","author":"Rodriguez","year":"1993","journal-title":"J. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3294","DOI":"10.1128\/JVI.01994-10","article-title":"Processing and Localization of the African Swine Fever Virus CD2v Transmembrane Protein","volume":"85","author":"Goatley","year":"2011","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e01293-18","DOI":"10.1128\/JVI.01293-18","article-title":"A Proteomic Atlas of the African Swine Fever Virus Particle","volume":"92","author":"Alejo","year":"2018","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.virusres.2019.04.002","article-title":"African Swine Fever Virus Evasion of Host Defences","volume":"266","author":"Dixon","year":"2019","journal-title":"Virus Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1099\/vir.0.80058-0","article-title":"Characterization of Pathogenic and Non-Pathogenic African Swine Fever Virus Isolates from Ornithodoros Erraticus Inhabiting Pig Premises in Portugal","volume":"85","author":"Boinas","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1016\/j.vaccine.2018.03.040","article-title":"African Swine Fever Virus (ASFV) Protection Mediated by NH\/P68 and NH\/P68 Recombinant Live-Attenuated Viruses","volume":"36","author":"Gallardo","year":"2018","journal-title":"Vaccine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e01058-17","DOI":"10.1128\/JVI.01058-17","article-title":"BA71\u2206CD2v: A new recombinant live attenuated African swine fever virus with cross-Protective Capabilities","volume":"91","author":"Monteagudo","year":"2017","journal-title":"J. Virol."},{"key":"ref_20","first-page":"494","article-title":"Deletion of CD2-like Gene from the Genome of African Swine Fever Virus Strain Georgia Does Not Attenuate Virulence in Swine","volume":"10","author":"Borca","year":"2020","journal-title":"Scientific"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hemmink, J.D., Abkallo, H.M., Henson, S.P., Khazalwa, E.M., Oduor, B., Lacasta, A., Okoth, E., Riitho, V., Fuchs, W., and Bishop, R.P. (2022). The African Swine Fever Isolate ASFV-Kenya-IX-1033 is Highly Virulent and Stable after Propagation in the Wild Boar Cell Line WSL. Viruses, 14.","DOI":"10.3390\/v14091912"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s11262-017-1521-4","article-title":"Multi-Locus Sequence Typing of African Swine Fever Viruses from Endemic Regions of Kenya and Eastern Uganda (2011\u20132013) Reveals Rapid B602L Central Variable Region Evolution","volume":"54","author":"Onzere","year":"2018","journal-title":"Virus Genes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.1016\/j.vaccine.2011.04.052","article-title":"Protection of European Domestic Pigs from Virulent African Isolates of African Swine Fever Virus by Experimental Immunisation","volume":"29","author":"King","year":"2011","journal-title":"Vaccine"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1038\/s41598-018-19626-1","article-title":"Efficient Inhibition of African Swine Fever Virus Replication by CRISPR\/Cas9 Targeting of the Viral P30 Gene (CP204L)","volume":"8","author":"Petersen","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0166-0934(02)00189-1","article-title":"Development of a TaqMan\u00ae PCR Assay with Internal Amplification Control for the Detection of African Swine Fever Virus","volume":"107","author":"King","year":"2003","journal-title":"J. Virol. Methods"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Iacolina, L., Penrith, M.-L., Bellini, S., Chenais, E., Jori, F., Montoya, M., St\u00e5hl, K., and Gavier-Wid\u00e9n, D. (2021). Understanding and Combatting African Swine Fever, Wageningen Academic Publishers.","DOI":"10.3920\/978-90-8686-910-7"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2016.11.021","article-title":"Different Routes and Doses Influence Protection in Pigs Immunised with the Naturally Attenuated African Swine Fever Virus Isolate OURT88\/3","volume":"138","author":"Chapman","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e00350-20","DOI":"10.1128\/JVI.00350-20","article-title":"Absence of Long-Term Protection in Domestic Pigs Immunized with Attenuated African Swine Fever Virus Isolate OURT88\/3 or Benin\u0394MGF Correlates with Increased Levels of Regulatory T Cells and Interleukin-10","volume":"94","author":"Jabbar","year":"2020","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1001\/jama.2015.12763","article-title":"Evaluation and Treatment of Pericarditis: A Systematic Review","volume":"314","author":"Imazio","year":"2015","journal-title":"JAMA J. Am. Med. Assoc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.actatropica.2018.06.025","article-title":"The Importance of On-Farm Biosecurity: Sero-Prevalence and Risk Factors of Bacterial and Viral Pathogens in Smallholder Pig Systems in Uganda","volume":"187","author":"Dione","year":"2018","journal-title":"Acta Trop."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40813-020-0144-7","article-title":"Status and Gaps of Research on Respiratory Disease Pathogens of Swine in Africa","volume":"6","author":"Oba","year":"2020","journal-title":"Porc. Health Manag."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1099\/vir.0.82857-0","article-title":"Systematic Analysis of Longitudinal Serological Responses of Pigs Infected Experimentally with African Swine Fever Virus","volume":"88","author":"Reis","year":"2007","journal-title":"J. Gen. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1917\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:16Z","timestamp":1760142016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/1917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":32,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["v14091917"],"URL":"https:\/\/doi.org\/10.3390\/v14091917","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}