{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:31:30Z","timestamp":1771655490683,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Municipal Natural Science Foundation","award":["6202003"],"award-info":[{"award-number":["6202003"]}]},{"name":"Beijing Municipal Natural Science Foundation","award":["31672597"],"award-info":[{"award-number":["31672597"]}]},{"name":"Beijing Municipal Natural Science Foundation","award":["KM202010020008"],"award-info":[{"award-number":["KM202010020008"]}]},{"name":"National Natural Science Foundation of China","award":["6202003"],"award-info":[{"award-number":["6202003"]}]},{"name":"National Natural Science Foundation of China","award":["31672597"],"award-info":[{"award-number":["31672597"]}]},{"name":"National Natural Science Foundation of China","award":["KM202010020008"],"award-info":[{"award-number":["KM202010020008"]}]},{"name":"Scientific Research Projects of Beijing Municipal Education Commission","award":["6202003"],"award-info":[{"award-number":["6202003"]}]},{"name":"Scientific Research Projects of Beijing Municipal Education Commission","award":["31672597"],"award-info":[{"award-number":["31672597"]}]},{"name":"Scientific Research Projects of Beijing Municipal Education Commission","award":["KM202010020008"],"award-info":[{"award-number":["KM202010020008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Our previous study has demonstrated that porcine pulmonary microvascular endothelial cells (MVECs) are susceptible to highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The innate immune response of MVECs infected with HP-PRRSV would play important roles in controlling virus proliferation, resisting cellular injury, and preventing the virus from spreading to other tissues and organs. Type I interferon is one of the most effective antiviral cytokines in the innate immune response, and interferon-induced proteins with tetratricopeptide repeats (IFITs) are members of interferon-stimulated genes induced by viruses and other pathogens, which are crucial in inhibiting virus proliferation and regulating the innate immune response. However, their effects on HP-PRRSV-induced innate immunity in porcine pulmonary MVECs remain unclear. Here, the roles of IFITs in porcine pulmonary MVECs infected with the HP-PRRSV HN strain were investigated, and the effects of astragalus polysaccharides (APS), a widely used traditional Chinese herbal ingredient with the immunopotentiating effect, on them were studied. The results showed that more autophagosomes were observed in HP-PRRSV-infected MVECs, and the expression of IFN-\u03b1, IFIT3, and IFIT5 decreased or increased at different time points after infection. When silencing the genes of IFIT3 or IFIT5, the HP-PRRSV replication in MVECs was significantly increased. The expression of IFIT3 and IFIT5 could be upregulated by APS, whose inhibitory effects on the HP-PRRSV replication significantly declined when the genes of IFIT3 or IFIT5 were silenced. The results suggest that IFIT3 and IFIT5 play an important role in inhibiting the HP-PRRSV replication in porcine pulmonary MVECs, and APS suppress the multiplication of HP-PRRSV by upregulating their expression.<\/jats:p>","DOI":"10.3390\/v14091919","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T02:09:36Z","timestamp":1661911776000},"page":"1919","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["IFIT3 and IFIT5 Play Potential Roles in Innate Immune Response of Porcine Pulmonary Microvascular Endothelial Cells to Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus"],"prefix":"10.3390","volume":"14","author":[{"given":"Yanmei","family":"Wu","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Defeng","family":"Cui","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2631-158X","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.vetmic.2017.02.020","article-title":"Pathogenesis and control of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus","volume":"209","author":"Han","year":"2017","journal-title":"Vet. 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