{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:54:09Z","timestamp":1780084449125,"version":"3.54.0"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA-IRTA Agreement","award":["8064-32000-060-14S"],"award-info":[{"award-number":["8064-32000-060-14S"]}]},{"name":"USDA-IRTA Agreement","award":["RTI2018-100887-B-I00"],"award-info":[{"award-number":["RTI2018-100887-B-I00"]}]},{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades from the Spanish government","award":["8064-32000-060-14S"],"award-info":[{"award-number":["8064-32000-060-14S"]}]},{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades from the Spanish government","award":["RTI2018-100887-B-I00"],"award-info":[{"award-number":["RTI2018-100887-B-I00"]}]},{"name":"Chinese government","award":["8064-32000-060-14S"],"award-info":[{"award-number":["8064-32000-060-14S"]}]},{"name":"Chinese government","award":["RTI2018-100887-B-I00"],"award-info":[{"award-number":["RTI2018-100887-B-I00"]}]},{"name":"CERCA Programme\/Generalitat de Catalunya","award":["8064-32000-060-14S"],"award-info":[{"award-number":["8064-32000-060-14S"]}]},{"name":"CERCA Programme\/Generalitat de Catalunya","award":["RTI2018-100887-B-I00"],"award-info":[{"award-number":["RTI2018-100887-B-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Control of classical swine fever virus (CSFV) in endemic countries relies on vaccination, mostly using vaccines that do not allow for differentiation of vaccinated from infected animals (DIVA). FlagT4G vaccine is a novel candidate that confers robust immunity and shows DIVA capabilities. The present study assessed the immune response elicited by FlagT4G and its capacity to protect pigs for a short time after vaccination. Five days after a single dose of FlagT4G vaccine, animals were challenged with a highly virulent CSFV strain. A strong, but regulated, interferon-\u03b1 response was found after vaccination. Vaccinated animals showed clinical and virological protection against the challenge, in the absence of antibody response at 5 days post-vaccination. Upon challenge, a rapid rise in the titers of CSFV neutralizing antibodies and an increase in the IFN-\u03b3 producing cells were noticed in all vaccinated-challenged pigs. Meanwhile, unvaccinated pigs showed severe clinical signs and high viral replication, being euthanized before the end of the trial. These animals were unable to generate neutralizing antibodies and IFN-\u03b3 responses after the CSFV challenge. The results from the present study assert the fast and efficient protection by FlagT4G, a highly promising tool for CSFV control worldwide.<\/jats:p>","DOI":"10.3390\/v14091954","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T20:50:27Z","timestamp":1662670227000},"page":"1954","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The FlagT4G Vaccine Confers a Strong and Regulated Immunity and Early Virological Protection against Classical Swine Fever"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6715-1757","authenticated-orcid":false,"given":"Jos\u00e9 Alejandro","family":"Boh\u00f3rquez","sequence":"first","affiliation":[{"name":"WOAH Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Bellaterra, Spain"},{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Barcelona, Spain"},{"name":"IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Barcelona, Spain"},{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61820, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Wang","sequence":"additional","affiliation":[{"name":"WOAH Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Bellaterra, Spain"},{"name":"Unitat Mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Barcelona, Spain"},{"name":"IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Barcelona, 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