{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:06:52Z","timestamp":1790651212394,"version":"4.1.0"},"reference-count":114,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T00:00:00Z","timestamp":1662854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Intramural Research Program of the National Institute of Allergy and Infectious Diseases (NIAID)"},{"name":"National Institutes of Health (NIH)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>An ongoing monkeypox outbreak in non-endemic countries has resulted in the declaration of a public health emergency of international concern by the World Health Organization (WHO). Though monkeypox has long been endemic in regions of sub-Saharan Africa, relatively little is known about its ecology, epidemiology, and transmission. Here, we consider the relevant research on both monkeypox and smallpox, a close relative, to make inferences about the current outbreak. Undetected circulation combined with atypical transmission and case presentation, including mild and asymptomatic disease, have facilitated the spread of monkeypox in non-endemic regions. A broader availability of diagnostics, enhanced surveillance, and targeted education, combined with a better understanding of the routes of transmission, are critical to identify at-risk populations and design science-based countermeasures to control the current outbreak.<\/jats:p>","DOI":"10.3390\/v14092012","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:15:02Z","timestamp":1663114502000},"page":"2012","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Atypical and Unique Transmission of Monkeypox Virus during the 2022 Outbreak: An Overview of the Current State of Knowledge"],"prefix":"10.3390","volume":"14","author":[{"given":"Jade C.","family":"Riopelle","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-3196","authenticated-orcid":false,"given":"Vincent J.","family":"Munster","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia R.","family":"Port","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.7326\/M22-1581","article-title":"A Novel International Monkeypox Outbreak","volume":"175","author":"Adalja","year":"2022","journal-title":"Ann. Intern. Med."},{"key":"ref_2","unstructured":"CDC (2022, September 01). Monkeypox and Orthopoxvirus Outbreak Global Map. Centers for Disease Control and Prevention, Available online: https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world-map.html."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.vetmic.2009.08.026","article-title":"Zoonotic poxviruses","volume":"140","author":"Essbauer","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Brown, K.L.P. (2016). Human Monkeypox: Current State of Knowledge and Implications for the Future. Trop. Med. Infect. Dis., 1.","DOI":"10.3390\/tropicalmed1010008"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Bunge, E.H.B., Chen, L., Lienert, F., Weidenthaler, H., Baer, L., and Steffen, R. (2022). The changing epidemiology of human monkeypox\u2014A potential threat? A systematic review. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010141"},{"key":"ref_6","unstructured":"WHO (2022). Multi-Country Monkeypox Outbreak in Non-Endemic Countries."},{"key":"ref_7","unstructured":"IA, C.H., Mbala, P., Njouom, R., Nakoune, E., Happi, A., Ndodo, N., Ayansola, O., Mboowa, G., Bedford, T., and Neher, R.A. (2022, September 01). Urgent Need for a Non-Discriminatory and Non-Stigmatizing Nomenclature for Monkeypox Virus. Available online: https:\/\/virological.org\/t\/urgent-need-for-a-non-discriminatory-and-non-stigmatizing-nomenclature-for-monkeypox-virus\/853\/1."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1099\/vir.0.81215-0","article-title":"A tale of two clades: Monkeypox viruses","volume":"86","author":"Likos","year":"2005","journal-title":"J. Gen. Virol."},{"key":"ref_9","first-page":"165","article-title":"Human monkeypox, 1970\u20131979","volume":"58","author":"Breman","year":"1980","journal-title":"Bull. World Health Organ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/infdis\/154.4.551","article-title":"Human monkeypox: A study of 2510 contacts of 214 patients","volume":"154","author":"Jezek","year":"1986","journal-title":"J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nm1273","article-title":"Multiple diagnostic techniques identify previously vaccinated individuals with protective immunity against monkeypox","volume":"11","author":"Hammarlund","year":"2005","journal-title":"Nat. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/S0140-6736(86)90748-8","article-title":"Isolation of monkeypox virus from wild squirrel infected in nature","volume":"327","author":"Khodakevich","year":"1986","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3201\/eid0703.017311","article-title":"Outbreak of Human Monkeypox, Democratic Republic of Congo, 1996\u20131997","volume":"7","author":"Hutin","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Doty, J.M.J., Kalemba, L., Stanley, W., Monroe, B., Nakazawa, Y., Mauldin, M., Bakambana, T., Liyandja, T., Braden, Z., and Wallace, R. (2017). Assessing Monkeypox Virus Prevalence in Small Mammals at the Human-Animal Interface in the Democratic Republic of the Congo. Viruses, 9.","DOI":"10.3390\/v9100283"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"746","DOI":"10.4269\/ajtmh.2010.09-0716","article-title":"A Silent Enzootic of an Orthopoxvirus in Ghana, West Africa: Evidence for Multi-Species Involvement in the Absence of Widespread Human Disease","volume":"82","author":"Reynolds","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/s10393-010-0355-5","article-title":"Using Remote Sensing to Map the Risk of Human Monkeypox Virus in the Congo Basin","volume":"8","author":"Fuller","year":"2010","journal-title":"EcoHealth"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Thomassen, H.F.T., Asefi-Najafabady, S., Shiplacoff, J., Mulembakani, P., Blumberg, S., Johnston, S., Kisalu, N., Kinkela, T., Fair, J., and Wolfe, N. (2013). Pathogen-Host Associations and Predicted Range Shifts of Human Monkeypox in Response to Climate Change in Central Africa. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066071"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/14787210.2019.1567330","article-title":"Monkeypox re-emergence in Africa: A call to expand the concept and practice of One Health","volume":"17","author":"Reynolds","year":"2019","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/S1473-3099(19)30294-4","article-title":"Outbreak of human monkeypox in Nigeria in 2017\u201318: A clinical and epidemiological report","volume":"19","author":"Dalhat","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_20","first-page":"31","article-title":"Serological survey for human monkeypox infections in a selected population in Zaire","volume":"90","author":"Jezek","year":"1987","journal-title":"J. Trop. Med. Hyg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3201\/eid2502.181222","article-title":"Epidemiologic and Ecologic Investigations of Monkeypox, Likouala Department, Republic of the Congo, 2017","volume":"25","author":"Doshi","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Falendysz, E.L.J., Doty, J., Nakazawa, Y., Crill, C., Lorenzsonn, F., Kalemba, L., Ronderos, M., Mejia, A., Malekani, J., and Karem, K. (2017). Characterization of Monkeypox virus infection in African rope squirrels (Funisciurus sp.). PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005809"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Falendysz, E.L.J., Lorenzsonn, F., Salzer, J., Hutson, C., Doty, J., Gallardo-Romero, N., Carroll, D., Osorio, J., and Rocke, T. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004130"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"426","DOI":"10.3201\/eid1003.030878","article-title":"Monkeypox Transmission and Pathogenesis in Prairie Dogs","volume":"10","author":"Guarner","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.3201\/eid2006.131329","article-title":"Fatal Monkeypox in Wild-Living Sooty Mangabey, Cote d\u2019Ivoire, 2012","volume":"20","author":"Radonic","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1038\/s41564-020-0706-0","article-title":"Monkeypox virus emergence in wild chimpanzees reveals distinct clinical outcomes and viral diversity","volume":"5","author":"Patrono","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Alakunle, E.M.U., Nchinda, G., and Okeke, M. (2020). Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution. Viruses, 12.","DOI":"10.3390\/v12111257"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"125","DOI":"10.7589\/2012-04-100","article-title":"Serologic evidence for circulating orthopoxviruses in peridomestic rodents from rural Uganda","volume":"49","author":"Salzer","year":"2013","journal-title":"J. Wildl. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"980","DOI":"10.3201\/eid2505.190076","article-title":"Diagnosis of Imported Monkeypox, Israel, 2018","volume":"25","author":"Erez","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1016\/S0140-6736(11)61142-2","article-title":"Maculopapular lesions in the Central African Republic","volume":"378","author":"Berthet","year":"2011","journal-title":"Lancet"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.3201\/eid2508.190112","article-title":"Intrafamily Transmission of Monkeypox Virus, Central African Republic, 2018","volume":"25","author":"Besombes","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","first-page":"747","article-title":"Monkeypox virus: Ecology and public health significance","volume":"66","author":"Khodakevich","year":"1988","journal-title":"Bull. World Health Organ."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"718","DOI":"10.4269\/ajtmh.15-0095","article-title":"Human Monkeypox in the Kivus, a Conflict Region of the Democratic Republic of the Congo","volume":"93","author":"McCollum","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1128\/JCM.40.8.2919-2921.2002","article-title":"Outbreaks of Disease Suspected of Being Due to Human Monkeypox Virus Infection in the Democratic Republic of Congo in 2001","volume":"40","author":"Meyer","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1056\/NEJMoa032299","article-title":"The detection of monkeypox in humans in the Western Hemisphere","volume":"350","author":"Reed","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Nalca, A.L.V., Garza, N., Zumbrun, E., Frick, O., Chapman, J., and Hartings, J. (2010). Experimental Infection of Cynomolgus Macaques (Macaca fascicularis) with Aerosolized Monkeypox Virus. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0012880"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/infdis\/124.1.33","article-title":"Induction, Course, and Transmissibilty of Monkeypox in the Baboon (Papio cynocephalus)","volume":"124","author":"Heberling","year":"1971","journal-title":"J. Infect. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"16262","DOI":"10.1073\/pnas.1005769107","article-title":"Major increase in human monkeypox incidence 30 years after smallpox vaccination campaigns cease in the Democratic Republic of Congo","volume":"107","author":"Rimoin","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Beer, E.R.B. (2019). A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007791"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/ije\/17.3.643","article-title":"The Transmission Potential of Monkeypox Virus in Human Populations","volume":"17","author":"Fine","year":"1988","journal-title":"Int. J. Epidemiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5077","DOI":"10.1016\/j.vaccine.2020.04.062","article-title":"Human monkeypox\u2013After 40 years, an unintended consequence of smallpox eradication","volume":"38","author":"Simpson","year":"2020","journal-title":"Vaccine"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.3201\/eid2704.203569","article-title":"Reemergence of Human Monkeypox and Declining Population Immunity in the Context of Urbanization, Nigeria, 2017\u20132020","volume":"27","author":"Nguyen","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.meegid.2019.01.006","article-title":"Monkeypox virus phylogenetic similarities between a human case detected in Cameroon in 2018 and the 2017\u20132018 outbreak in Nigeria","volume":"69","author":"Yonga","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"ofx168","DOI":"10.1093\/ofid\/ofx168","article-title":"A Nosocomial Outbreak of Human Monkeypox in the Central African Republic","volume":"4","author":"Nakoune","year":"2017","journal-title":"Open Forum Infect. Dis."},{"key":"ref_45","first-page":"459","article-title":"Clinico-epidemiological features of monkeypox patients with an animal or human source of infection","volume":"66","author":"Jezek","year":"1988","journal-title":"Bull. World Health Organ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"410","DOI":"10.4269\/ajtmh.15-0168","article-title":"Introduction of Monkeypox into a Community and Household: Risk Factors and Zoonotic Reservoirs in the Democratic Republic of the Congo","volume":"93","author":"Nolen","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Quiner, C.M.C., Monroe, B., Nakazawa, Y., Doty, J., Hughes, C., McCollum, A., Ibata, S., Malekani, J., Okitolonda, E., and Carroll, D. (2017). Presumptive risk factors for monkeypox in rural communities in the Democratic Republic of the Congo. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0168664"},{"key":"ref_48","first-page":"1022","article-title":"Transmission of monkeypox among persons exposed to infected prairie dogs in Indiana in 2003","volume":"159","author":"Kile","year":"2005","journal-title":"JAMA Pediatr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1800509","DOI":"10.2807\/1560-7917.ES.2018.23.38.1800509","article-title":"Two cases of monkeypox imported to the United Kingdom, September 2018","volume":"23","author":"Vaughan","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2100745","DOI":"10.2807\/1560-7917.ES.2021.26.32.2100745","article-title":"Family cluster of three cases of monkeypox imported from Nigeria to the United Kingdom, May 2021","volume":"26","author":"Hobson","year":"2021","journal-title":"Eurosurveillance"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.3201\/eid2608.191387","article-title":"Imported Monkeypox, Singapore","volume":"26","author":"Yong","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_52","unstructured":"Health, M.D. (2021). Travel-Associated Monkeypox Virus Infection Confirmed in Maryland Resident, Maryland Department of Health Newsroom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"638","DOI":"10.2471\/BLT.19.242347","article-title":"Modelling human-to-human transmission of monkeypox","volume":"98","author":"Grant","year":"2020","journal-title":"Bull. World Health Organ"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Leendertz, S.S.D., Theophil, D., Anoh, E., Mossoun, A., Schubert, G., Wiersma, L., Akoua-Koffi, C., Couacy-Hymann, E., Muyembe-Tamfum, J., and Karhemere, S. (2017). A Cross-Sectional Serosurvey of Anti-Orthopoxvirus Antibodies in Central and Western Africa. Viruses, 9.","DOI":"10.3390\/v9100278"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1038\/414748a","article-title":"Transmission potential of smallpox in contemporary populations","volume":"414","author":"Gani","year":"2001","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1093\/aje\/kwg103","article-title":"Transmission Potential of Smallpox: Estimates Based on Detailed Data from an Outbreak","volume":"158","author":"Eichner","year":"2003","journal-title":"Am. J. Epidemiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/nature04153","article-title":"Superspreading and the effect of individual variation on disease emergence","volume":"438","author":"Kopp","year":"2005","journal-title":"Nature"},{"key":"ref_58","first-page":"304","article-title":"Human Monkeypox\u2013Kasai Oriental, Zaire, 1996\u20131997","volume":"46","author":"Mwamba","year":"1997","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Blumberg, S., and Lloyd-Smith, J.O. (2013). Inference of R(0) and transmission heterogeneity from the size distribution of stuttering chains. PLoS Comput. Biol., 9.","DOI":"10.1371\/journal.pcbi.1002993"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2200421","DOI":"10.2807\/1560-7917.ES.2022.27.22.2200421","article-title":"Epidemiological, clinical, and virological characteristics of four cases of monkeypox support transmission through sexual contact, Italy, May 2022","volume":"27","author":"Antinori","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/S1473-3099(22)00228-6","article-title":"Clinical features and management of human monkeypox: A retrospective observational study in the UK","volume":"22","author":"Adler","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_62","first-page":"2200503","article-title":"Frequent detection of monkeypox virus DNA in saliva, semen, and other clinical samples from 12 patients, Barcelona, Spain, May to June 2022","volume":"27","author":"Fuertes","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1056\/NEJMra020025","article-title":"Diagnosis and Management of Smallpox","volume":"346","author":"Breman","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"ref_64","unstructured":"CDC (2022, September 08). How does smallpox spread? Centers for Disease Control and Prevention. 7 June 2016, Available online: https:\/\/www.cdc.gov\/smallpox\/transmission\/index.html."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1093\/infdis\/jix260","article-title":"Maternal and Fetal Outcomes Among Pregnant Women With Human Monkeypox Infection in the Democratic Republic of Congo","volume":"216","author":"Mbala","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1136\/bmj.1.4348.628","article-title":"Aerial Convection from Smallpox Hospitals","volume":"1","author":"Millard","year":"1944","journal-title":"Br. Med. J."},{"key":"ref_67","first-page":"669","article-title":"An airborne outbreak of smallpox in a German hospital and its significance with respect to other recent outbreaks in Europe","volume":"43","author":"Wehrle","year":"1970","journal-title":"Bull. World Health Organ."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Hutson, C.C.D., Gallardo-Romero, N., Weiss, S., Clemmons, C., Hughes, C., Salzer, J., Olson, V., Abel, J., Karem, K., and Damon, I. (2011). Monkeypox Disease Transmission in an Experimental Setting: Prairie Dog Animal Model. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028295"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.virol.2010.03.012","article-title":"Dosage comparison of Congo Basin and West African strains of monkeypox virus using a prairie dog animal model of systemic orthopoxvirus disease","volume":"402","author":"Hutson","year":"2010","journal-title":"Virology"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Hutson, C.G.-R.N., Carroll, D., Clemmons, C., Salzer, J., Nagy, T., Hughes, C., Olson, V., Karem, K., and Damon, I. (2013). Transmissibility of the Monkeypox Virus Clades via Respiratory Transmission: Investigation Using the Prairie Dog-Monkeypox Virus Challenge System. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055488"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1086\/505880","article-title":"Clinical Manifestations of Human Monkeypox Influenced by Route of Infection","volume":"194","author":"Reynolds","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1093\/infdis\/125.2.161","article-title":"Smallpox in Europe, 1950\u20131971","volume":"125","author":"Mack","year":"1972","journal-title":"J. Infect. Dis."},{"key":"ref_73","first-page":"49","article-title":"Studies on the virus content of mouth washings in the acute phase of smallpox","volume":"25","author":"Downie","year":"1961","journal-title":"Bull. World Health Organ."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.3201\/eid2206.150579","article-title":"Extended Human-to-Human Transmission during a Monkeypox Outbreak in the Democratic Republic of the Congo","volume":"22","author":"Nolen","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"782","DOI":"10.3201\/eid2604.191164","article-title":"Human-to-Human Transmission of Monkeypox Virus, United Kingdom, October 2018","volume":"26","author":"Vaughan","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Ogoina, D.I.J., Ogunleye, A., Ederiane, E., Anebonam, U., Anebonam, U., Neni, A., Oyeyemi, A., Etebu, E.N., and Ihekweazu, C. (2019). The 2017 human monkeypox outbreak in Nigeria-report of outbreak experience and response in the Niger Delta University Teaching Hospital, Bayelsa State, Nigeria. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0214229"},{"key":"ref_77","unstructured":"CDC (1963). Smallpox\u2013Stockholm, Sweden, 1963, Morbidity and Mortality Weekly Report."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0022-2143(03)00147-1","article-title":"Rates and risks of transmission of smallpox and mechanisms of prevention","volume":"142","author":"Kiang","year":"2003","journal-title":"J. Lab. Clin. Med."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Judson, S.M.V. (2019). Nosocomial Transmission of Emerging Viruses via Aerosol-Generating Medical Procedures. Viruses, 11.","DOI":"10.3390\/v11100940"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"428","DOI":"10.4269\/ajtmh.2005.73.428","article-title":"Extended interhuman transmission of monkeypox in a hospital community in the Republic of the Congo, 2003","volume":"73","author":"Learned","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_81","unstructured":"(2022, September 08). Monkeypox and Smallpox Vaccine Guidance. CDC. 2 June 2022, Available online: https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/clinicians\/smallpox-vaccine.html."},{"key":"ref_82","unstructured":"(2022, September 08). Considerations for Monkeypox Vaccination. CDC 22 August 2022, Available online: https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/health-departments\/vaccine-considerations.html."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"734","DOI":"10.15585\/mmwr.mm7122e1","article-title":"Use of JYNNEOS (Smallpox and Monkeypox Vaccine, Live, Nonreplicating) for Preexposure Vaccination of Persons at Risk for Occupational Exposure to Orthopoxviruses: Recommendations of the Advisory Committee on Immunization Practices-United States, 2022","volume":"71","author":"Rao","year":"2022","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"D54","DOI":"10.1016\/j.vaccine.2011.04.014","article-title":"Status of human monkeypox: Clinical disease, epidemiology, and research","volume":"29","author":"Damon","year":"2011","journal-title":"Vaccine"},{"key":"ref_85","unstructured":"(2022, September 08). Epidemiological Update: Monkeypox Multi-Country Outbreak. European Centre for Disease Prevention and Control. Available online: https:\/\/www.ecdc.europa.eu\/en\/news-events\/epidemiological-update-monkeypox-multi-country-outbreak-15-june."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Guzzetta, G., Mammone, A., Ferraro, F., Caraglia, A., Rapiti, A., Marziano, V., Poletti, P., Cereda, D., Vairo, F., and Mattei, G. (2022). Early Estimates of Monkeypox Incubation Period, Generation Time, and Reproduction Number, Italy, May\u2013June 2022. Emerg. Infect. Dis., 28.","DOI":"10.3201\/eid2810.221126"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Du, Z., Shao, Z., Bai, Y., Wang, L., Herrera-Diestra, J.L., Fox, S.J., Ertem, Z., Lau, E.H.Y., and Cowling, B.J. (2022). Reproduction number of monkeypox in the early stage of the 2022 multi-country outbreak. J. Travel Med., taac099. Online ahead of print.","DOI":"10.1101\/2022.07.26.22278042"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1038\/s41591-022-01907-y","article-title":"Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus","volume":"28","author":"Isidro","year":"2022","journal-title":"Nat. Med."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/S0140-6736(22)01487-8","article-title":"Evidence of human-to-dog transmission of monkeypox virus","volume":"400","author":"Seang","year":"2022","journal-title":"Lancet"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Edgar, R.C. (2004). MUSCLE: A multiple sequence alignment method with reduced time and space complexity. BMC Bioinform., 5.","DOI":"10.1186\/1471-2105-5-113"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: Fast model selection for accurate phylogenetic estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat Methods"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.15585\/mmwr.mm7132e3","article-title":"Epidemiologic and Clinical Characteristics of Monkeypox Cases-United States, 17 May\u201322 July, 2022","volume":"71","author":"Philpott","year":"2022","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Bragazzi, N.K.J., Mahroum, N., Tsigalou, C., Khamisy-Farah, R., Converti, M., and Wu, J. (2022). Epidemiological trends and clinical features of the ongoing mokeypox epidemic: A preliminary pooled data analysis and literature review. J. Med. Virol.","DOI":"10.1002\/jmv.27931"},{"key":"ref_96","first-page":"145","article-title":"Secondary and Tertiary Transmission of Vaccinia Virus after Sexual Contact with a Smallpox Vaccinee\u2014San Diego, California, 2012","volume":"62","author":"Shao","year":"2013","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/S1473-3099(22)00513-8","article-title":"Monkeypox virus isolation from a semen sample collected in the early phase of infection in a patient with prolonged seminal viral shedding","volume":"22","author":"Lapa","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_98","first-page":"2200424","article-title":"Ongoing monkeypox virus outbreak, Portugal, 29 April to 23 May 2022","volume":"27","author":"Ribeiro","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_99","unstructured":"CDC (2022, August 31). Demographics of Patients Receiving TPOXX for Treatment of Monkeypox 2022, Available online: https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/demographics-TPOXX.html."},{"key":"ref_100","first-page":"2200552","article-title":"Paediatric monkeypox patient with unknown source of infection, the Netherlands, June 2022","volume":"27","author":"Fievez","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Endo, A.M.H., Abbott, S., Ratnayake, R., Pearson, C., Edmunds, W., Fearon, E., and Funk, S. (2022). Heavy-tailed sexual contact networks and the epidemiology of monkeypox outbreak in non-endemic regions, May 2022. MedRxiv.","DOI":"10.1101\/2022.06.13.22276353"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"e643","DOI":"10.1016\/S2666-5247(22)00183-5","article-title":"Projected burden and duration of the 2022 Monkeypox outbreaks in non-endemic countries","volume":"3","author":"Bisanzio","year":"2022","journal-title":"Lancet Microbe"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.jinf.2022.05.028","article-title":"Transmission of monkeypox virus through sexual contact\u2013A novel route of infection","volume":"85","author":"Heskin","year":"2022","journal-title":"J. Infect."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"764","DOI":"10.15585\/mmwr.mm7123e1","article-title":"Monkeypox Outbreak\u2013Nine States, May 2022","volume":"71","author":"Minhaj","year":"2022","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1016\/S1473-3099(22)00411-X","article-title":"Demographic and Clinical Characteristics of Confirmed Human Monkeypox Virus Cases in Individuals Attending a Sexual Health Centre in London, United Kingdom: An Observational Analysis","volume":"22","author":"Girometti","year":"2022","journal-title":"Lancet Inf. Dis."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Charniga, K.M.N., Slayton, R., Gosdin, L., Minhaj, F., Philpott, D., Smith, D., Gearhart, S., Alvarado-Ramy, F., Brown, C., and Waltenburg, M.A. (2022). Estimating the incubation period of monkeypox virus during the 2022 multi-national outbreak. medRxiv.","DOI":"10.1101\/2022.06.22.22276713"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"200448","DOI":"10.2807\/1560-7917.ES.2022.27.24.2200448","article-title":"Estimated incubation period for monkeypox cases confirmed in the Netherlands, May 2022","volume":"27","author":"Miura","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Ogoina, D., and Yinka-Ogunleye, A. (2022). Sexual history of human monkeypox patients seen at a tertiary hospital in Bayelsa, Nigeria. Int. J. STD AIDS, 1\u20135.","DOI":"10.1177\/09564624221119335"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1093\/cid\/ciaa143","article-title":"Clinical Course and Outcome of Human Monkeypox in Nigeria","volume":"71","author":"Ogoina","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Ferr\u00e9, V.M., Bachelard, A., Zaidi, M., Armand-Lefevre, L., Descamps, D., Charpentier, C., and Ghosn, J. (Ann. Intern. Med., 2022). Detection of Monkeypox Virus in Anorectal Swabs From Asymptomatic Men Who Have Sex With Men in a Sexually Transmitted Infection Screening Program in Paris, France, Ann. Intern. Med., Online ahead of print.","DOI":"10.7326\/M22-2183"},{"key":"ref_111","unstructured":"(2022, September 08). National Technical Guidelines for Integrated Disease Surveillance and Response. Monrovia. Available online: https:\/\/www.afro.who.int\/sites\/default\/files\/2021-09\/Final_IDSR%20Technical%20Guidelines_Final%20Version%20signed%20by%20%20Hon.%20DG%20and%20Hon.%20Minister.pdf."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2200603","DOI":"10.2807\/1560-7917.ES.2022.27.33.2200603","article-title":"Monkeypox self-diagnosis abilities, determinants of vaccination and self-isolation intention after diagnosis among MSM, the Netherlands, July 2022","volume":"27","author":"Wang","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"e011453","DOI":"10.1136\/bmjopen-2016-011453","article-title":"Examining the associations between HIV-related stigma and health outcomes in people living with HIV\/AIDS: A series of meta-analyses","volume":"6","author":"Rueda","year":"2016","journal-title":"BMJ Open"},{"key":"ref_114","unstructured":"Mendos, L.R. (2019). State-Sponsored Homophobia 2019, International Lesbian, Gay, Bisexual, Trans and Intersex Association."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/2012\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:29:27Z","timestamp":1760142567000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/2012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,11]]},"references-count":114,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["v14092012"],"URL":"https:\/\/doi.org\/10.3390\/v14092012","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints202208.0128.v1","asserted-by":"object"}]},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,11]]}}}