{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T14:03:55Z","timestamp":1778076235432,"version":"3.51.4"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the World Organisation for Animal Health (OIE) through the European Union","award":["FOOD\/2016\/379-660"],"award-info":[{"award-number":["FOOD\/2016\/379-660"]}]},{"name":"the World Organisation for Animal Health (OIE) through the European Union","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"the World Organisation for Animal Health (OIE) through the European Union","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"the Chinese Academy of Sciences, a Shanghai Municipal Science and Technology Major Project","award":["FOOD\/2016\/379-660"],"award-info":[{"award-number":["FOOD\/2016\/379-660"]}]},{"name":"the Chinese Academy of Sciences, a Shanghai Municipal Science and Technology Major Project","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"the Chinese Academy of Sciences, a Shanghai Municipal Science and Technology Major Project","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"the Ministry of Science and Technology of China","award":["FOOD\/2016\/379-660"],"award-info":[{"award-number":["FOOD\/2016\/379-660"]}]},{"name":"the Ministry of Science and Technology of China","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"the Ministry of Science and Technology of China","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Yellow fever remains a public-health threat in remote regions of Africa. Here, we report the identification and genetic characterisation of one yellow-fever case observed during the investigation of a cluster of nine suspected haemorrhagic fever cases in a village in the Central African Republic. Samples were tested using real-time RT-PCR targeting the main African haemorrhagic fever viruses. Following negative results, we attempted virus isolation on VERO E6 cells and new-born mice and rescreened the samples using rRT-PCR. The whole viral genome was sequenced using an Illumina NovaSeq 6000 sequencer. Yellow-fever virus (YFV) was isolated from one woman who reported farming activities in a forest setting several days before disease onset. Phylogenetic analysis shows that this strain belongs to the East\u2013Central African YFV genotype, with an estimated emergence some 63 years ago. Finally, five unique amino-acid changes are present in the capsid, envelop, NS1A, NS3, and NS4B proteins. More efforts are required to control yellow-fever re-emergence in resource-limited settings.<\/jats:p>","DOI":"10.3390\/v14092014","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:15:02Z","timestamp":1663114502000},"page":"2014","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2083-3280","authenticated-orcid":false,"given":"Huguette","family":"SIMO TCHETGNA","sequence":"first","affiliation":[{"name":"Centre for Research in Infectious Diseases, Yaound\u00e9 P.O. Box 13591, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2036-6176","authenticated-orcid":false,"given":"St\u00e9phane","family":"DESCORPS-DECLERE","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Bioinformatics and Biostatistics Hub, 75006 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"SELEKON","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"GARBA-OUANGOLE","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"KONAMNA","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathieu","family":"SOUNGOUZA","sequence":"additional","affiliation":[{"name":"Centre de Sant\u00e9 de Mboki, Mboki, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaspard","family":"TEKPA","sequence":"additional","affiliation":[{"name":"Service d\u2019Infectiologie, H\u00f4pital de l\u2019Amiti\u00e9, Bangui, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"SOMSE","sequence":"additional","affiliation":[{"name":"Ministry of Health, Bangui P.O. Box 883, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"NAKOUNE","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"BERTHET","sequence":"additional","affiliation":[{"name":"Cellule d\u2019Intervention Biologique d\u2019Urgence, Unit\u00e9 Environnement et Risque Infectieux, Institut Pasteur, 75015 Paris, France"},{"name":"Unit of Discovery and Molecular Characterization of Pathogens, Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"ref_1","unstructured":"WHO (2021, May 13). Yellow Fever. 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